Nucleic acid screening method of staphylococcus aureus, salmonella, shigella and listeria monocytogenes
A technology for mononucleosis and staphylococcus, which is applied in the field of microbiological testing, can solve the problems of poor specificity, high laboratory safety requirements, and long time, and achieve the effect of simple method
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[0039] Example
[0040] (1) Nucleic acid extraction: aspirate 1ml of bacterial suspension from the bacterial suspension cultured on a shaker at 37°C for 10 hours, centrifuge at 10000rpm for 5min, discard the supernatant, add 100μl of sterile water, shake well (or store it in a refrigerator at 4°C) Pick 2-3 colonies on the plate and place them in 100 μl sterile water). Boil at 100°C for 10 minutes, and centrifuge at 10000 rpm for 5 minutes in a benchtop centrifuge. The supernatant is genomic DNA, which can be directly used as a template for nucleic acid amplification reactions.
[0041] (2) LAMP reaction: prepare the above reaction system, mix the reaction system, and dispense it into 0.2ml PCR reaction tubes, 19ul per tube, add the template extracted in step (1) to the PCR reaction tubes, 1ul per tube , 1ul each of the positive control substance and the negative control substance, respectively, as a positive control and a negative control, mix well. Put it in a constant temperatu...
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