Method for improving activity of malt amylase
A technology of amylase and malt powder, applied in the directions of biochemical equipment and methods, enzymes, hydrolase, etc., can solve the problems of cost limitation and limitation, and achieve the effect of reducing raw material cost, simple technology, saving energy and resources
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Embodiment 1
[0024] The first step is to dry the barley malt in an oven at 40°C for 24 hours;
[0025] In the second step, the above-mentioned dried barley malt is pulverized by a DL F U disc grinder, and passed through a sieve with an aperture of 80 mesh for subsequent use;
[0026] The third step is to add a phosphate buffer solution with a pH of 5.8 to the malt powder that has been sieved in the second step. Centrifuge for 10 min, take the supernatant, and obtain the crude extract of malt amylase;
[0027] The fourth step is to use the JY92-2D ultrasonic cell pulverizer to ultrasonically treat the crude extract of malt amylase. The ultrasonic conditions are: ultrasonic frequency 20kHz, ultrasonic power 120W, pulse mode 1:1 (ultrasonic time 3s, stop 3s), ultrasonic radiation The total time is 15 minutes, and the temperature of the ultrasonic system is 3°C. According to DNS (3,5-dinitrosalicylic acid) method test and analysis, its amylase activity reaches 1238.932U / g, which is 27.17% hi...
Embodiment 2
[0029] The first step is to dry the barley malt in an oven at 35°C for 36 hours to remove excess water;
[0030] The second step is to use the ZN-08 laboratory small-scale pulverizer to pulverize the above-mentioned dried barley malt, and pass it through a 40-mesh sieve for subsequent use;
[0031] The third step is to add a phosphate buffer solution with a pH of 5.8 to the malt powder after the sieving treatment in the second step, and the concentration is controlled to be 1:10g / ml, shake and mix well, place it at room temperature for 60min, and then centrifuge at 1500r / min for 15min, and take supernatant to obtain crude extract of malt amylase;
[0032] The fourth step is to use the JY92-2D ultrasonic cell pulverizer to perform ultrasonic treatment on the crude extract of malt amylase. The total time of irradiation is 10 minutes, and the temperature of the ultrasonic system is 2°C. Its amylase activity reaches 1202.596U / g by DNS (3,5-dinitrosalicylic acid) method test and ...
Embodiment 3
[0034] Step 1 Dry the barley malt in an oven at 38°C for 30 hours to remove excess moisture.
[0035] The second step is to grind the above-mentioned dried barley malt with a grinding cup, and pass through a 60-mesh sieve for subsequent use;
[0036] The third step is to add phosphate buffer solution with a pH of 5.8 to the malt powder after the sieving treatment in the second step. supernatant to obtain crude extract of malt amylase;
[0037] The fourth step is to use BILON-1200Y ultrasonic cell pulverizer to carry out ultrasonic treatment to the crude extract of malt amylase. The ultrasonic conditions are: the fixed ultrasonic frequency is 20kHz, the ultrasonic power is 120W, the total time of ultrasonic radiation is 20min, and the pulse mode is 1:3 (ultrasonic radiation Time 3s, stop 9s), ultrasonic system temperature 3 ℃. Its amylase activity reaches 1209.982U / g through DNS (3,5-dinitrosalicylic acid) method test and analysis, which is 24.20% higher than maltogenase acti...
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