Para-thiocyano benzyl five-nitrogen heterocyclic ring tetraacethyl chelate and preparation method thereof

A kind of technology of isothiocyanatobenzyl pentaazine, chelate compound, applied in the application of immunoassay, p-isothiocyanatobenzyl pentaazacyclotetraacetic acid chelate compound and preparation thereof, homogeneous time-resolved fluorescence Immunoassay reagent field
CN101906077AInactive Publication Date: 2010-12-08INST OF RADIATION MEDICINE CHINESE ACADEMY OF MEDICAL SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
INST OF RADIATION MEDICINE CHINESE ACADEMY OF MEDICAL SCI
Publication Date
2010-12-08
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a para-thiocyano benzyl five-nitrogen heterocyclic ring tetraacethyl chelate and a preparation method thereof. The preparation method comprises the following steps of: condensing, hydrolyzing, substituting, and condensing the product with bromoacetic acid; then, chelating with lanthanides to obtain para-nitro benzyl five-nitrogen heterocyclic ring tetraacethyl, and reducing with 5% Pd / C; and finally, reacting with thiophosgene to obtain a target compound I. The para-thiocyano benzyl five-nitrogen heterocyclic ring tetraacethyl chelate is an unknown compound, the formed rigid cryptate is chelated with the lanthanides of europium, indium, samarium, terbium and the like, and the structure is determined by elementary analysis, infrared spectra, ultraviolet spectra, nuclear magnetism and mass spectra. The prepared chelate is mainly used for time-resolved fluoroimmunoassay.
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Description

technical field

[0001] The invention belongs to the technical field of synthesis of organic heterocyclic compounds, and relates to a p-isothiocyanatobenzyl pentaazacyclotetraacetic acid chelate and a preparation method thereof, and the chelate is used as a homogeneous time-resolved fluorescent immunoassay reagent for immunoassay Analysis application. Background technique

[0002] The time-resolved fluorescence immunoassay has been rapidly developed and widely used because of its high sensitivity, rapidity, the use of automatic analytical instruments and the development of technology. The method uses lanthanide elements as markers to label antibodies (or antigens), oligonucleotides, biomacromolecules, target cells, etc., and then perform immune reactions of antigens and antibodies to generate immune complexes, or perform nucleic acid hybridization analysis , DNA sequence detection, etc. The principle is that lanthanide ions such as europium, samarium, terbium, and dysprosium...

Claims

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