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Xanthomonas, preparation method thereof and method thereof for producing temperature resistant xanthan gum polysaccharide

A technology of xanthomonas and xanthan gum, applied in the field of xanthomonas, can solve the problems of product quality decline, poor temperature resistance, unstable viscosity of aqueous solution, etc., and achieve product viscosity increase, good temperature resistance, Effect of improving temperature resistance

Active Publication Date: 2010-12-08
ORDOS ZHONGXUAN BIOCHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the new technology of "polymer flooding" developed in recent years, the amount of xanthan gum accounts for about 30% to 40% of the polymer, but due to the poor temperature resistance of the existing Xanthomonas strains, some produced The viscosity of xanthan gum products drops rapidly at high temperature, which affects the application in some aspects, and the existing xanthan gum-based biological polysaccharide products also generally have unstable viscosity in aqueous solution, and product quality declines under high temperature conditions, etc. Therefore, it is very important to develop a temperature-resistant Xanthomonas strain and produce high-temperature-resistant microbial polysaccharides

Method used

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Experimental program
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Effect test

Embodiment 1

[0046] (1) Breeding of temperature-resistant strains

[0047] ①. Preparation of Xanthomonas bacterial suspension:

[0048] Inoculate Xanthomonas in seed shake flasks, culture with shaking at 28±1°C for 18-24 hours, centrifuge the seed solution several times and wash it with buffer to make a bacterial suspension, in which the seed medium is The components are proportioned by weight with 1% sucrose, 0.3% beef extract, and 0.5% yeast powder, and the pH is adjusted to 7.0±0.2.

[0049] ②. Nitrosoguanidine mutagenesis

[0050] Add dissolved nitrosoguanidine to the bacterial suspension and treat it for 1-1.5 hours, centrifuge and wash the treated bacterial suspension several times, then dilute and spread it on a plate, and cultivate it at 36-38°C for three days.

[0051] ③. UV mutagenesis

[0052] Pick strong and good colonies after nitrosoguanidine mutagenesis, culture them in shake flasks, and then make a bacterial suspension. Take 5ml of the bacterial suspension and place it ...

Embodiment 2

[0057] (1) Expanded cultivation of seeds:

[0058] ①. First-class seed tank:

[0059] Put the components of the seed medium into the first-level seed tank with water in turn according to the weight ratio (1% sucrose, 0.3% beef extract, and 0.5% yeast powder), stir evenly to dissolve, and adjust the pH to 7.0±0.2 ;Using real disinfection, the temperature is 121°C, the pressure is 0.1Mpa, and the time is 45 minutes; when the temperature is lowered to 28±1°C, under sterile conditions, inoculate at a rate of 1% from the inoculation port, and the bacteria after inoculation are at 28±1°C Under certain conditions, cultivate for 24 hours, use a biological microscope to detect the growth of the bacteria, and replant when the number of bacteria is large and the growth is full;

[0060] ②. Secondary seed tank:

[0061] Put the components of the seed medium into the secondary seed tank with water in turn according to the weight ratio (1% sucrose, 0.3% beef extract, 0.5% yeast powder), s...

Embodiment 3

[0065] (1) Expanded cultivation of seeds:

[0066] ①. First-class seed tank:

[0067] Put the components of the seed medium into the first-level seed tank with water in turn according to the weight ratio (sucrose 1.2%, beef extract 0.4%, yeast powder 0.6%), stir evenly to dissolve, adjust the pH to 7.0±0.2 ;Using real disinfection, the temperature is 121°C, the pressure is 0.12Mpa, and the time is 50 minutes; when the temperature is lowered to 28±1°C, under sterile conditions, the inoculation port is inoculated at a ratio of 1.2%, and the bacteria after inoculation are at 28±1°C Under the conditions of 20 hours, use a biological microscope to detect the growth of the bacteria, and when the number of bacteria is large and the growth is full, replant;

[0068] ②. Secondary seed tank:

[0069] Put the components of the seed medium into the secondary seed tank with water in turn according to the weight ratio (sucrose 1.2%, beef extract 0.4%, yeast powder 0.6%), stir evenly to di...

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Abstract

The invention discloses a xanthomonas Xanthomonassp. S-96#, preparation method thereof and a method thereof for producing temperature resistant xanthan gum polysaccharide, including seed selection of temperature resistant high viscosity strain, expanding culture of seed, fermentation and extraction processing steps. The strain of the invention has good temperature resistance, the produced xanthan gum polysaccharide product has stable quality, the viscosity of the product after being heated is obviously higher than similar product, application indexes are excellent, and the original production process of similar products is improved, production process is safe, thus being capable of substituting the existing xanthan gum product and being widely applied to the petroleum development industries requiring high viscosity under high temperature condition.

Description

technical field [0001] The invention relates to a Xanthomonas sp. S-96#, in particular to a Xanthomonas sp. S-96#, a preparation method and a method for producing temperature-resistant xanthan gum polysaccharides by using the Xanthomonas sp. Background technique [0002] Due to the rheological characteristics, salt resistance and thickening effect of microbial fermentation polysaccharides, it has obvious technical advantages in oilfield development compared with polyacrylamide, CMC, modified starch and some polysaccharide plants (guar gum, etc.). . Among them, xanthan gum is a kind of microbial exopolysaccharide with a wide range of functions produced by Xanthomonas bacteria with carbohydrates as the main raw material through fermentation engineering. As an excellent oilfield drilling mud additive, its application is called One of the latest achievements in mud technology in the 1970s, its unique high viscosity under low shear conditions enables low-concentration drilling f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N15/01C12N13/00C12P19/06C12R1/64
Inventor 王新纲司书锋孙正艳陈健
Owner ORDOS ZHONGXUAN BIOCHEM
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