The invention discloses a safe use method for preparing a medical ultrasonic coupling agent sterile product, wherein the method comprises the following steps: S1, putting an unsterilized medical ultrasonic coupling agent material into a stainless steel barrel, and carrying out steam sterilization, wherein the formula of the medical ultrasonic coupling agent material comprises 0.5-1.5% of carbomer,3-5% of propylene glycol, 1-3% of glycerol, 1-2% of triethanolamine, and the balance being purified water; S2, after sterilization, cooling the medical ultrasonic coupling agent material to 25 DEG C-80 DEG C, and packaging, wherein a packaging bag is an aluminum composite film packaging bag, the aluminum composite film packaging bag is of a four-layer film structure, and four layers of films sequentially comprise a polyester layer, an aluminum foil layer, a polyamide or Kevlar layer and a polyethylene or polypropylene layer from outside to inside; and S3, after packaging is completed, carrying out terminal thermal sterilization. For facilitating the use of an operating table, a layer of specially-made paper-plastic bag is sleeved on an aluminum composite film packaging bag of a common sterile product, and then sterilization is carried out again. Compared with other methods, the safe use method is simple to operate, good in product quality and low in production cost.