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Method for producing paratyphus living vaccine for piglets

A technology for piglet paratyphoid fever and production methods, which is applied in the direction of medical formulas, medical preparations containing active ingredients, non-active ingredients of polymer compounds, etc., and can solve the problems of large influence of type, age, freshness and degree of digestion, low survival rate, Issues such as instability between batches

Active Publication Date: 2012-08-22
北京中海生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But there are still the following outstanding problems: 1. The vaccine uses ordinary broth as the medium, and the quality of the main component of the medium, the beef infusion, is greatly affected by the type, age, freshness and degree of digestion of the beef, resulting in instability between batches
2. The vaccine uses 1.5% gelatin and 5% sucrose as protective agents, the formula is simple, the survival rate of freeze-drying is low, and the protective function is poor (especially at higher temperatures)
The low survival rate makes the vaccine contain a large number of dead bacteria, and the endotoxin produced by the disintegration of dead bacteria is one of the factors that cause allergic reactions. In addition, low temperature storage brings difficulties to storage, transportation and use in remote areas

Method used

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  • Method for producing paratyphus living vaccine for piglets
  • Method for producing paratyphus living vaccine for piglets
  • Method for producing paratyphus living vaccine for piglets

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Inoculate the seed solution of Salmonella choleraesuis (CVCC79500 strain) in the synthetic medium (liquid medium) provided by the present invention according to 1% to 2% of the total medium amount, and ferment or aerate for 18~21h at 37°C. Add an appropriate amount of antifoaming agent as needed, and add an appropriate amount of sterilized 40% glucose to control the pH value according to the increase in pH. After the cultivation, centrifuge at 3500r / min for 20min, remove the supernatant, immediately add the sterilized and preheated to 37°C heat-resistant freeze-drying protective agent provided by the present invention, mix well, and dispense according to the regulations. Should pay attention to insulated and shaken evenly in the subpackaging process, and carry out freeze vacuum drying rapidly, press the method that " Chinese Veterinary Pharmacopoeia " stipulates, use freeze-drying curve 2 that the present invention provides (see figure 2 ), the number of live bacteria i...

Embodiment 2

[0056] Inoculate the seed liquid of Salmonella choleraesuis (strain CVCC79500) in common broth medium (formulation recorded in "Chinese Veterinary Pharmacopoeia") according to 1% to 2% of the total medium, ferment or aerate at 37°C for 18 to 21 hours, During the cultivation process, an appropriate amount of antifoaming agent was added as needed, and an appropriate amount of sterilized 40% glucose was added according to the pH rise to control the pH value. After the cultivation, centrifuge at 3500r / min for 20min, remove the supernatant, immediately add the sterilized and preheated heat-resistant protective agent to 37°C, mix thoroughly, and dispense according to the prescribed portion. Should pay attention to insulated and shaken evenly in the subpackaging process, and carry out freeze vacuum drying rapidly, press the method that " Chinese Veterinary Pharmacopoeia " stipulates, use freeze-drying curve 2 that the present invention provides (see figure 2 ), the number of live bac...

Embodiment 3

[0058] Product testing

[0059] Appearance White spongy loose mass, easy to separate from the bottle wall, dissolve quickly after adding diluent.

[0060] The pure test shall be tested according to the method stipulated in the "Chinese Veterinary Pharmacopoeia", and it shall be pure.

[0061] The number of live bacteria should be marked according to the bottle label, and the number of live bacteria should be counted on an ordinary agar plate (according to the method stipulated in the "Chinese Veterinary Pharmacopoeia"). The number of live bacteria in each head should not be less than 3×10 9 CFU.

[0062] Safety inspection According to the label on the bottle, mark the dose, dilute the vaccine with ordinary broth or peptone water, inject subcutaneously into 2 rabbits weighing 1.5-2.0kg, each 1.0ml (including 2 doses), observe for 21 days, and should survive.

[0063] The remaining moisture shall be determined according to the method stipulated in "Chinese Veterinary Pharmacop...

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Abstract

The invention relates to a method for producing a paratyphus living vaccine for piglets. In the method of the invention, a whole set of technology for synthesizing culture mediums, lyophilized heat-resisting protective agents and adaptive lyophilization curves of the lyophilized heat-resisting protective agents is adopted. Therefore, the method for producing the paratyphus living vaccine for the piglets can improve the number of cultured bacterial strain of salmonella suipestifer CVCC79500, ensure the stability among batches of vaccines, reduce the loss rate of the living vaccine in the process of lyophilization, reduce bacterial death and an anaphylactic reaction which is caused by catabolites, increase the storage temperature of finished vaccines to 2 to 8 DEG C, make the valid period reach 18 to 24 months, and realize the short-term storage of the paratyphus living vaccine at a high temperature (the paratyphus living vaccine for the piglets can be kept for 7 days at the temperatureof 37 DEG C, and the decrement rate of the living vaccines is less than 20 percent).

Description

technical background [0001] Piglet paratyphus (Paratyphus swine), also known as swine salmonellosis (Swine salmonellosis), is mainly a high-heat infectious disease of piglets caused by Salmonella choleraesuis, and is currently mainly prevented by vaccination. [0002] Piglet paratyphoid fever is mainly caused by Salmonella choleraesuis septicemia and enteritis in young pigs and shelf pigs. It is mostly a recessive carrier for adult pigs or a secondary infection of swine fever, and occasionally causes food poisoning in humans and damages other animals. [0003] Piglet paratyphoid live vaccine CVCC500 strain (formerly C500 strain) used in China (Fang Xiaowen, Li Yanglong, Huang Changbing, Zheng Ming, Feng Wenda, Sun Wei, etc. Research on attenuated paratyphoid vaccine for piglets, Journal of Animal Husbandry and Veterinary Medicine, 1979) , has strong immunity to piglets, stable and safe virulence, and plays an important role in the prevention and control of swine salmonellosis....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/112A61K47/42A61P31/04
Inventor 朱良全王栋孙晔刘延亭
Owner 北京中海生物科技有限公司
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