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Extraction technology of gambogic acid

An extraction process, the technology of gambogic acid, which is applied in the field of medicine, can solve the problems of unfavorable industrial scale-up production, cumbersome operation steps, and slow processing process, and achieve the effects of recyclable solvent, high purity, and fast extraction speed

Active Publication Date: 2013-04-24
LIAONING UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage is that the operation steps are cumbersome, the operation process uses a variety of solvents, it is difficult to recover, the processing process is slow, and the extraction yield is low, which is not conducive to industrial scale-up production

Method used

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  • Extraction technology of gambogic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A kind of extraction process of gambogic acid of embodiment 1

[0018] Proceed as follows:

[0019] (1) Weigh 6 kg of garcinia cambogia, crush it into 50 mesh powder, add 12 kg of silica gel (the silica gel is pretreated in advance: add 8000 mL of methanol to the silica gel to wash the silica gel until dry. Then wash the silica gel with 8000 mL ethyl acetate until dry ) were evenly mixed, filled into a chromatographic column with a diameter of 50 cm, and eluted with ethyl acetate. Concentrate under reduced pressure at 40°C and recover the solvent to obtain the initial yellow gambogic acid extract.

[0020] (2) Dissolve the primary extract of gambogic acid in pyridine (mass volume (g:ml) ratio 1:2), shake fully for 1 hour, add 1 times the volume of the mixture in 50°C distilled water, stir well, let stand, and cool Solids were precipitated, filtered by suction and dried to obtain 1.89Kg of the crude product of pyridinium salt of gambogic acid.

[0021] (3) Dissolve th...

Embodiment 2

[0024] A kind of extraction process of gambogic acid of embodiment 2

[0025] Proceed as follows:

[0026] (1) Weigh 6 kg of gamboge, crush it into 30 mesh powder, add 24 kg of diatomaceous earth and mix evenly, fill it into a chromatographic column with a diameter of 80 cm, and elute with methanol. The eluent is initially dark brown, and the eluent is Stop elution when pale yellow, collect the eluate, concentrate the eluate under reduced pressure at 40°C and recover the solvent to obtain a yellow paste-like gambogic acid primary extract.

[0027] (2) Dissolve the initial gambogic acid extract in triethylamine (mass volume (g:ml) ratio 2:1), shake fully for 1 hour, add distilled water with 4 times the volume of the mixture and stir fully for 2 hours, after standing Solids were precipitated, filtered by suction, and dried to obtain 1.98Kg of the crude product of gambogic acid triethylamine salt.

[0028] (3) The obtained gambogic acid triethylamine salt crude product is disso...

Embodiment 3

[0031] Embodiment 3 A kind of extraction process of gambogic acid

[0032] Proceed as follows:

[0033] (1) Weigh 6 kg of gamboge resin, grind it into 60 mesh powder; add 6 kg of quartz powder (quartz powder is pretreated in advance: add 8000 mL of methanol to the quartz powder to wash the quartz powder until dry. Then wash with 8000 mL of ethyl acetate Quartz powder, to dryness) uniformly mixed, filled into a chromatographic column with a diameter of 50 cm, and eluted with an organic solvent (the volume ratio of methanol to dichloromethane is 1:1), the eluent is initially dark brown, and the eluent is Stop elution when it is pale yellow, collect the eluate, concentrate the eluate under reduced pressure at 40°C and recover the solvent to obtain the yellow primary extract of gambogic acid.

[0034] (2) dissolving the gambogic acid primary extract in pyridine (mass volume (g:ml) ratio 1:3), fully shaking for 1 hour, adding distilled water with 2 times the volume of the mixture ...

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Abstract

The invention relates to an extraction technology of gambogic acid, which comprises the following steps of firstly, crushing gamboge, uniformly mixing with silica, diatomite, quartz powder or polyacrylamide the quantity of which is 0.5-5 times as much as that of the crushed gamboge, installing a chromatography column, adding an organic solvent, washing, collecting eluent, concentrating the eluent, and recovering a solvent to obtain a gambogic acid primary extractive; secondly, dissolving the gambogic acid primary extractive into triethylamine or pyridine, fully oscillating to form a salt, adding distilled water, stirring, standing, pump filtering, and drying to obtain a crude gambogic acid salt product; thirdly, dissolving the obtained crude gambogic acid salt product into the solvent to prepare a saturated solution, crystallizing to obtain gambogic acid salt crystals, pump filtering, and drying to obtain gambogic acid salts; fourthly, extracting the gambogic acid salts with hydrochloric acid and diethyl ether, separating liquid, taking a diethyl ether layer, reducing the pressure, recovering a diethyl ether solution, and drying to finally obtain solid gambogic acid. The inventionhas the advantages of simple steps, recycled solvent, rapid extraction speed, high purity and high yield.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to a process for extracting gambogic acid, an active ingredient derived from plants, which can inhibit the growth of tumor cells. Background technique [0002] Gambogic acid is a compound extracted from Garcinia cambogia with high anti-tumor activity and good cell selectivity. It has low solubility in water and high solubility in organic solvents such as alcohols and ketones. . Garcinia cambogia is a dry resin secreted by the Garcinia cambogia tree. At present, more than 20 gambogic acid compounds have been isolated and identified from gamboge, and the commercially available gamboge is cylindrical or irregular solid, brittle and brittle, with a yellowish-brown and waxy luster. The solid is red-yellow or orange-brown, and the powder is bright yellow. Garcinia contains gambogic acid, neogambogic acid, allogambogic acid and other components. [0003] In the prior art, the Chines...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D493/20
Inventor 王洋徐利锋刘举陈烨周云鹏张秋实刘阳
Owner LIAONING UNIVERSITY