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Microbial purifying agent for aquaculture water and preparation method thereof

A technology for aquaculture water and microorganisms, applied in biological water/sewage treatment, chemical instruments and methods, water/sludge/sewage treatment, etc., which can solve the problem that free Bacillus coagulans cannot settle naturally, are easily eaten by other organisms, and limit restoration. problems such as the pond substrate, to achieve effective adsorption, prolong the repair cycle, and not easy to run off

Active Publication Date: 2012-12-26
ZHEJIANG GONGSHANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current research focuses on free Bacillus coagulans, because it is easily washed away by water flow under flowing water conditions, and easily ingested by other organisms under static water conditions, and free Bacillus coagulans cannot naturally settle into the water-sediment of the culture pond water body interface, limiting its ability to repair pond substrates

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Preparation of activated montmorillonite: according to the weight ratio of montmorillonite:distilled water=1:10, the montmorillonite was stirred evenly in distilled water, then left to soak for 36 hours, and dispersed in water to form a suspension. After passing through a 300-mesh sieve, let it stand still, settle for 24 hours, extract the suspension at a depth of 15 cm below the liquid surface, and obtain activated montmorillonite with a particle size of less than 0.5 μm after centrifugal drying.

[0017] According to the ratio of Bacillus coagulans bacterial liquid in the logarithmic growth phase: activated montmorillonite = 1:2 (volume to mass ratio), mixed culture under aerobic conditions, cultured at 30-35°C for 24h, and air-dried at room temperature to obtain fixed spores coagulans Bacillus montmorillonite particles, stored at room temperature.

[0018] In the early stage of shrimp culture, according to 100g / m 2 Sprinkle the above-mentioned water purifying agent ...

Embodiment 2

[0021] Preparation of activated montmorillonite: according to the weight ratio of montmorillonite:distilled water=1:20, the montmorillonite was stirred evenly in distilled water, then left to soak for 24 hours, and dispersed in water to form a suspension. After passing through a 300-mesh sieve, let it stand still, settle for 24 hours, extract the suspension with a depth of more than 10 cm, and obtain activated montmorillonite with a particle size of less than 0.5 μm after centrifugal drying.

[0022] According to the ratio of Bacillus coagulans bacterial liquid in the logarithmic growth phase: activated montmorillonite = 1:1 (volume to mass ratio), mixed culture under aerobic conditions, cultured at 30-35°C for 36h, and air-dried at room temperature to obtain fixed Bacillus coagulans Bacillus montmorillonite particles, stored at room temperature.

[0023] In the later stage of shrimp culture, according to 75g / m 2 Sprinkle the above-mentioned water purifying agent evenly in th...

Embodiment 3

[0026] Preparation of activated montmorillonite: according to the weight ratio of montmorillonite:distilled water=1:15, the montmorillonite was stirred evenly in distilled water, then left to soak for 30 hours, and dispersed in water to form a suspension. After passing through a 200-mesh sieve, let it stand still, settle for 24 hours, extract the suspension at a depth of 15 cm, and obtain activated montmorillonite with a particle size of less than 0.5 μm after centrifugal drying.

[0027] According to the ratio of Bacillus coagulans bacterial liquid in logarithmic growth phase:activated montmorillonite=1:2 (volume to mass ratio), mixed culture under aerobic conditions, cultivated at 30~35°C for 36h, and obtained coagulans fixed after air-drying at room temperature Bacillus montmorillonite particles, stored at room temperature.

[0028] According to 50g / m during soft-shelled turtle breeding 2 Sprinkle the above-mentioned water purifying agent evenly in the soft-shelled turtle ...

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Abstract

The invention discloses a microbial purifying agent for aquaculture waterand the purifying agent is activated montmorillonite particles with immobilized bacillus coagulans. The invention also provides a preparation method of the purifying agent. The purifying agent uses activated montmorillonite as the immobilized carrier and uses bacillus coagulans as the immobilized object to proliferate through coculture; the quantity of the immobilized bacteria is high, the enrichment efficiency is high; the immobilized bacillus coagulans can maintain high activity, be protected by the activated montmorillonite from being eaten by other creatures and fast fall to the water-sediment interface of the bottom of a pond to purify water; the activated montmorillonite has better adsorption property and can more effectively adsorb the pollutants at the water-sediment interface; the used materials are non-toxic and harmless and can not cause secondary pollution; and the purifying agent has low cost and is convenient to transport and store, and the scale production of the purifying agent can be realized.

Description

technical field [0001] The invention belongs to the field of water body purification, and relates to a microbial water purifier for aquaculture water and a preparation method thereof. Background technique [0002] The deterioration of the breeding environment has become a key factor restricting the healthy and sustainable development of my country's aquaculture industry. Therefore, there is an urgent need to seek a method and technology for rehabilitating the pond culture environment that is effective and highly operable. Traditional physical or chemical methods mainly adopt the forms of adsorption, precipitation, filtration, and drug disinfection. Using physical or chemical methods not only has disadvantages such as incomplete purification, poor reuse rate, and high input costs, but also drug residues such as antibiotics and The emergence of drug-resistant strains in aquatic animals will eventually lead to the safety of aquatic products. In recent years, the method of usi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/34
Inventor 王彦波励建荣李学鹏
Owner ZHEJIANG GONGSHANG UNIVERSITY