Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparing ibuprofen-degrading bacterial agent

A technology of degrading bacteria and inoculation amount, which is applied in the field of preparation of ibuprofen degrading bacteria agent, can solve the problems of enhanced toxicity, achieve the effects of easy inactivation, protection of the ecological environment, and broad application prospects

Inactive Publication Date: 2011-01-26
DONGHUA UNIV
View PDF4 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The photo-Fenton method degrades the product of ibuprofen, but the toxicity is enhanced

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing ibuprofen-degrading bacterial agent
  • Method for preparing ibuprofen-degrading bacterial agent
  • Method for preparing ibuprofen-degrading bacterial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Isolation and identification of strains

[0028] (1) The activated sludge obtained from the secondary settling tank of the urban sewage treatment plant is inoculated in the inorganic salt culture medium by 5% of the access amount, domesticated with the gradient pressure type domestication method, and the concentration of ibuprofen is 10, 15, 20, 25, 30, 35 mg / L, acclimatized culture at 30°C, 160r / min, 250mL conical flask filling volume 100mL, the total acclimatization time is 2 months. Inorganic salt medium formula is K 2 HPO 4 4.35g, KH 2 PO 4 1.7g, NH 4 Cl 2.1g, MgSO 4 0.2g, MnSO 4 0.05g, FeSO 4 ·7H 2 O0.01g, CaCl 2 2H 2 O 0.03g, distilled water 1000mL, pH=6.8-7.0.

[0029] (2) Take the sample after 2 months of acclimatization, let it stand for 30 minutes, take 1mL of the upper layer water sample, serially dilute it, and streak it on the plate for separation. Then cultured in a 30°C incubator for 48h. A single colony with a clear shape was picked, ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for preparing an ibuprofen-degrading bacterial agent, which comprises the following steps of: (1) inoculating activated sludge in a secondary sedimentation tank of a sewage treatment plant into a culture medium, domesticating by using a gradient pressure domestication method, standing, taking an upper-layer water sample, gradually diluting, streaking for separation, culturing, purifying, inoculating the separated strain into a beef extract peptone culture medium, and performing shake culture until a logarithmic phase; and (2) inoculating the cultured strain into the culture medium to prepare the ibuprofen-degrading bacterial agent. The method has the advantages of simpleness and low cost; and the prepared ibuprofen-degrading bacterial agent has the advantages of convenient use, high efficiency of degrading ibuprofen, easy inactivation of the strain, and application to ibuprofen removal in water treatment.

Description

technical field [0001] The invention belongs to the field of preparation of ibuprofen-degrading bacterial agent, in particular to a preparation method of ibuprofen-degrading bacterial agent. Background technique [0002] Pharmaceutical and Personal Care Products (PPCPs for short) as a new kind of pollutants have been paid more and more attention by people. Drugs and personal care products, even in trace amounts, still have a considerable degree of biological activity, causing unpredictable impacts on the environment, and may pose potential hazards to human health through drinking water and food. [0003] Among the common PPCPs compounds, antibiotics, anti-inflammatory drugs, antiepileptic drugs, and blood lipid regulating drugs were frequently detected in various environmental media. Among them, non-steroidal antipyretic analgesic and anti-inflammatory drug ibuprofen (Ibuprofen, molecular structure such as figure 1 ) is widely used as an over-the-counter drug and is curren...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/12C02F3/34C12N1/20C12R1/43C02F101/34
CPCY02W10/10
Inventor 杨兴薛罡赵晓祥刘亚男史锦程起跃周宁娟孙超田世烜罗来盛阮俊杰
Owner DONGHUA UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com