Micro-ecological preparation and preparation method thereof
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A technology of micro-ecological preparations and strains, applied in application, animal feed, animal feed, etc., can solve problems such as loss of activity, and achieve the effects of reducing feed cost, strong tolerance, and improving growth performance
Inactive Publication Date: 2011-02-09
广东省农业科学院兽医研究所 +1
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However, both Lactobacillus and Bifidobacterium have a common fatal weakness, that is, they are easily affected by various external biochemical and physical factors during storage and oral administration and lose their activity, so they cannot achieve the expected purpose.
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preparation example Construction
[0027] The preparation method of above-mentioned probiotics comprises the following steps:
[0028] 1) Inoculate the butyric acid bacteria strains into the modified GAM semi-solid medium containing agar, and perform anaerobic culture to obtain the slant strains;
[0029] 2) Inoculate the slant strains into the liquid culture medium and perform anaerobic culture to obtain the liquid strains;
[0030] 3) Inoculate the liquid strain into the sterilized basic medium, and perform anaerobic fermentation for 48-60 hours, control the concentration of residual sugar in the fermentation process to not be higher than 0.5%, and control the amount of ammonia nitrogen to 0.05-0.15%;
[0031] 4) After the cultivation is completed, put it in a tank, mix it with the carrier, and spray dry it to obtain the probiotics;
[0032] Among them, the basic medium is: glucose 1%, peptone 0.5%, beef extract 0.3%, MgSO 4 ·7H 2 O0.05%, MnSO 4 ·H 2 O 0.02%, K 2 HPO 4 0.4%, NaCl 0.2%, CaCO 3 1%, th...
Embodiment
[0036] Inoculate the butyric acid bacteria strains into the modified GAM broth semi-solid medium containing 0.7% agar, and culture them anaerobically at 36°C for 24 hours to obtain the slant strains;
[0037] Inoculate the strains from the slant into the liquid culture medium and culture them anaerobically at 36°C for 24 hours to obtain the liquid strains;
[0038] Inoculate the liquid strains into the sterilized basic medium, anaerobic fermentation for 48 to 60 hours, control the feeding of 40% glucose solution and mixed nitrogen source during the fermentation process, and ensure that the residual sugar in the medium is not higher than 0.5%, the amount of ammonia nitrogen is controlled at 0.05-0.15%;
[0039] After the cultivation is completed, put it in a tank, mix it with a carrier, and spray dry it to obtain a microecological preparation;
[0040] Among them, the basic medium is: glucose 1%, peptone 0.5%, beef extract 0.3%, MgSO 4 ·7H 2 O0.05%, MnSO 4 ·H 2 O 0.02%, K ...
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Abstract
The invention discloses a micro-ecological preparation and a preparation method thereof. The micro-ecological preparation consists of viable clostridium butyricum and an acceptable carrier. The preparation method comprises the following steps of: inoculating clostridium butyricum strains into an improved agar-containing GAM semisolid culture medium and performing anaerobic cultivation to obtain slant stains; inoculating the slant strains into a liquid culture medium and performing the anaerobic cultivation to obtain liquid strains; inoculating the liquid strains into a sterilized basic culture medium, and performing anaerobic fermentation cultivation for 48 to 60 hours in a way of controlling residual sugar content in the fermentation process to be not over 0.5 percent and controlling ammonia nitrogen content to be 0.05 to 0.15 percent; and after the cultivation is finished, placing the liquid strains into a tank, mixing the liquid strains and the carrier and performing spray drying to obtain the micro-ecological preparation. The micro-ecological preparation of the invention can promote the growth of livestock and poultry, improve the immunity and improve the quality of animal products, and is favorable for reducing the cost of feeds.
Description
technical field [0001] The invention relates to a feed additive and a preparation method thereof, in particular to a butyric acid bacteria microecological preparation and a preparation method thereof. Background technique [0002] Feed additives refer to small or trace substances added during feed production, processing and use, which are used in small amounts but have significant effects in feed. Feed additive is an inevitable raw material used in the modern feed industry. It has obvious effects on strengthening the nutritional value of basic feed, improving the production performance of livestock and poultry, ensuring animal health, saving feed cost, and improving the quality of livestock products. [0003] Excellent feed additives not only require to continue to improve the nutrition, palatability and biological properties of feed, but more importantly, to solve the problem of feed safety. The safety of feed mainly includes three aspects: one is to eliminate the impact o...
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