Application of bdellovibrio mixture as bactericide to cleaning of fruits
A technology of Bdellovibrio and mixture, which is applied in the application field of Bdellovibrio mixture as a bactericide in cleaning fruits, can solve the problems of ineffective removal of microorganisms, pollution of water bodies, atmosphere, and inability to kill microorganisms, etc., to achieve Long-lasting bactericidal action, health protection, and fast onset effect
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Embodiment 1
[0039] Elimination of Common Pathogenic Bacteria on Grape Surface
[0040] (1) Preparation of Bdellovibrio Mixture Bacteria Liquid Eliminating Pathogenic Bacteria on the Grape Surface
[0041] The Bdellovibrio sp. BDM01 was deposited in China Center for Type Culture Collection on April 28, 2008, and the preservation number is CCTCC NO: M208066;
[0042] The Bdellovibrio BDM01 is obtained by ultraviolet mutagenesis of Bdellovibrio BDJ01, and its morphology is observed under an electron microscope after negative staining: Bdellovibrio BDM01 is single-celled, arc-shaped, 1.7×1.0 μm in size, terminal Flagella, the length of the flagella is 3.5 μm; it can form a transparent circular plaque with a diameter of 3-4 mm when it is cultured at 28 ° C for three days with a double-layer plate method.
[0043] The preparation of Bdellovibrio brucoplasma: inoculate 10mL 10 10 cfu / mL Aeromonas hydrophila (Aeromonas hydrophila, purchased from the Culture Collection Center of Guangdong Instit...
Embodiment 2
[0064] Elimination of Common Pathogenic Bacteria on the Surface of Fresh Jujube
[0065] Step (1), (2) and (3) are all the same as embodiment 1
[0066] Test results such as image 3 , 4 shown
[0067] Depend on image 3 It can be seen that at 10pfu / mL concentration of Bdellovibrio, the elimination rate of pathogenic bacteria can reach more than 97%, but the elimination effect of different combinations is also different. After the 30min experiment, the pathogenic bacteria in the bacterial liquid C group can hardly be detected. However, the number of pathogenic bacteria that can be detected in groups D and E is 3-4cfu / mL, and that in groups A and B is higher. The elimination rate of the 1:1 mixture can reach more than 99% for Escherichia coli, Salmonella typhimurium, Listeria monocytogenes and Staphylococcus aureus.
[0068] Depend on Figure 4 As shown, 1, 2, 3, and 5 in the figure represent 10, 10 respectively 2 、10 3 、10 5 pfu / mL (that is, the above-mentioned X is 1...
Embodiment 3
[0071] Comparison of Bactericidal Effects of New Fungicides and Traditional Fungicides
[0072] Step (1) and (2) are all the same as embodiment 1
[0073] Step (3) different fungicides to the elimination test of pathogenic bacteria
[0074] All the loquats in the control group and the experimental group were first sterilized by 75% alcohol, then soaked in the mixed bacterial solution prepared in step (2) for 1 min, taken out, drained, and then sterilized with different fungicides.
[0075] Loquat was selected as the effect fruit, and divided into experimental group and control group, with 5 loquats about 100g in each group.
[0076] Control group: take three loquats as three parallel samples, soak the loquats in tap water for 30 minutes;
[0077] Experimental group: divided into four groups, each group took three loquats, as three parallel samples
[0078] (1) Soak the loquat in tap water containing a concentration of 10pfu / mL mixed bacterial solution D
[0079] (2) loquat i...
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