Application of bdellovibrio mixture as bactericide to cleaning of fruits

A technology of Bdellovibrio and mixture, which is applied in the application field of Bdellovibrio mixture as a bactericide in cleaning fruits, can solve the problems of ineffective removal of microorganisms, pollution of water bodies, atmosphere, and inability to kill microorganisms, etc., to achieve Long-lasting bactericidal action, health protection, and fast onset effect

Inactive Publication Date: 2011-02-23
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the industrial cleaning process of fruits is mainly carried out by the combination of physical cleaning and chemical fungicides. First, different cleaning equipment is selected according to different types of fruits, such as DT5A1 cleaning machine, blast cleaning machine, XGJ-2 type The main principle of the washing machine is the friction and interaction between the fruit and the washing water. At the same time, it is assisted by the brush roller to clean the dirt on the fruit surface; secondly, choose a suitable cleaning agent. There are many types of cleaning agents on the market. For washing, decontamination, and sterilizing functions, although the traditional cleaning method is good for removing soil from fruits and vegetables, it has no obvious effect on microorganism removal
Most of the currently used fruit fungicides are chlorine-based fungicides, but these can not only kill microorganisms such as Escherichia coli, Salmonella, Listeria monocytogenes, Staphylococcus aureus, etc. on the surface of fruits, but also interact with certain ingredients in food The reaction produces chloride, which is harmful to the human body. The cleaned wastewater contains chloroform formed by the reaction of chlorine residues and organic matter in the water, which not only pollutes the water body and the atmosphere, but also causes cancer to the human body.

Method used

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  • Application of bdellovibrio mixture as bactericide to cleaning of fruits
  • Application of bdellovibrio mixture as bactericide to cleaning of fruits
  • Application of bdellovibrio mixture as bactericide to cleaning of fruits

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Elimination of Common Pathogenic Bacteria on Grape Surface

[0040] (1) Preparation of Bdellovibrio Mixture Bacteria Liquid Eliminating Pathogenic Bacteria on the Grape Surface

[0041] The Bdellovibrio sp. BDM01 was deposited in China Center for Type Culture Collection on April 28, 2008, and the preservation number is CCTCC NO: M208066;

[0042] The Bdellovibrio BDM01 is obtained by ultraviolet mutagenesis of Bdellovibrio BDJ01, and its morphology is observed under an electron microscope after negative staining: Bdellovibrio BDM01 is single-celled, arc-shaped, 1.7×1.0 μm in size, terminal Flagella, the length of the flagella is 3.5 μm; it can form a transparent circular plaque with a diameter of 3-4 mm when it is cultured at 28 ° C for three days with a double-layer plate method.

[0043] The preparation of Bdellovibrio brucoplasma: inoculate 10mL 10 10 cfu / mL Aeromonas hydrophila (Aeromonas hydrophila, purchased from the Culture Collection Center of Guangdong Instit...

Embodiment 2

[0064] Elimination of Common Pathogenic Bacteria on the Surface of Fresh Jujube

[0065] Step (1), (2) and (3) are all the same as embodiment 1

[0066] Test results such as image 3 , 4 shown

[0067] Depend on image 3 It can be seen that at 10pfu / mL concentration of Bdellovibrio, the elimination rate of pathogenic bacteria can reach more than 97%, but the elimination effect of different combinations is also different. After the 30min experiment, the pathogenic bacteria in the bacterial liquid C group can hardly be detected. However, the number of pathogenic bacteria that can be detected in groups D and E is 3-4cfu / mL, and that in groups A and B is higher. The elimination rate of the 1:1 mixture can reach more than 99% for Escherichia coli, Salmonella typhimurium, Listeria monocytogenes and Staphylococcus aureus.

[0068] Depend on Figure 4 As shown, 1, 2, 3, and 5 in the figure represent 10, 10 respectively 2 、10 3 、10 5 pfu / mL (that is, the above-mentioned X is 1...

Embodiment 3

[0071] Comparison of Bactericidal Effects of New Fungicides and Traditional Fungicides

[0072] Step (1) and (2) are all the same as embodiment 1

[0073] Step (3) different fungicides to the elimination test of pathogenic bacteria

[0074] All the loquats in the control group and the experimental group were first sterilized by 75% alcohol, then soaked in the mixed bacterial solution prepared in step (2) for 1 min, taken out, drained, and then sterilized with different fungicides.

[0075] Loquat was selected as the effect fruit, and divided into experimental group and control group, with 5 loquats about 100g in each group.

[0076] Control group: take three loquats as three parallel samples, soak the loquats in tap water for 30 minutes;

[0077] Experimental group: divided into four groups, each group took three loquats, as three parallel samples

[0078] (1) Soak the loquat in tap water containing a concentration of 10pfu / mL mixed bacterial solution D

[0079] (2) loquat i...

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Abstract

The invention discloses application of a bdellovibrio mixture as a bactericide to cleaning of fruits. The application is characterized by comprising the following steps of: adding the mixture bacterium fluid of bdellovibrio plastids and swimmers of bdellovibrio serving as the bactericide into water for cleaning, and cleaning the fruits; or spraying the mixture bacterium fluid of the bdellovibrio in fruit processing environment and cleaning the processing environment, wherein the bdellovibrio is Bdellovibrio sp. BDM01, is called CCTCC for short, and was collected in China Center for Type Culture Collection on April 28th, 2008 with the collection number of CCTCC NO: M208066. The mixed bacteria serving as the bactericide is utilized to clean the fruits, and 95 percent of pathogenic bacteria on the surface of the fruits can be killed. And the mixed bacteria have the advantages that: the swimmers have quick response and high activity, the bdellovibrio plastids are easy to prepare and store, the sterilization action time is longer and the environmental tolerance is higher.

Description

technical field [0001] The invention relates to the application of the Bdellovibrio mixture, in particular to the application of eliminating pathogenic bacteria carried on the fruit surface and its processing environment. Background technique [0002] Fruits contain rich nutrients necessary for human survival. Although eating vegetables can replace fruits to a large extent, they cannot be completely replaced, because the nutritional content of fruits is not the same as that of vegetables. Therefore, the consumption of fruits is very important for the comprehensive intake of human nutrition. Regular consumption of various fruits is one of the important means to ensure good nutrition and material balance in the body. [0003] With the continuous improvement of people's living standards, the consumption of fruit is no longer satisfied with the original eating method, and the quantity and quality of deep-processed fruit products, such as juice, jam, fruit wine and preserves, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/212A23L1/015C12N1/20C12R1/01A23L5/20A23L19/00
Inventor 蔡俊鹏陈丽芸
Owner SOUTH CHINA UNIV OF TECH
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