Drum fermentation reactor-based bacterial cellulose production technology

Abstract

The invention relates to drum fermentation reactor-based bacterial cellulose production technology, which comprises the following steps of: (1) sterilizing hollow equipment; (2) sterilizing culture medium; (3) inoculating; (4) performing rotary fermentation; (5) harvesting bacterial cellulose films; and (6) cleaning equipment. The bacterial cellulose production technology breaks through the limitation that the traditional tray culture method is performed manually and has low production efficiency, realizes continuous and mechanical work of bacterial cellulose fermentation production and film preparation, has the advantages of high treatment capacity, high efficiency, convenience, high degree of automation and the like, and can effectively improve the labor productivity, reduce the production cost and stabilize the product quality.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to a bacterial cellulose production process based on a drum type fermentation reactor. Background technique [0002] Cellulose is the most abundant natural polymer on the earth. It exists widely in plants such as trees and cotton. The cellulose produced by plants reaches hundreds of millions of tons every year. However, the synthesis of cellulose is not a unique function of plants. Some bacteria also produce extracellular cellulose more efficiently than plants in a heterotrophic way. We call this bacterial-derived cellulose "bacterial cellulose". Bacterial synthetic cellulose was first reported by Brown in 1886. It is a layer of white fibrous substance formed by Acetobacter xylinum on the surface of the medium during static culture. Bacterial cellulose production was later also found in many Gram-negative bacteria such as Agrobacterium, Pseudomonas tumefa...

AI Technical Summary

Problems solved by technology

However, the high cost caused by its low output limits its wide application.

Traditional bacterial cellulose production is mainly shallow pan fermentation, which is characterized by labor-intensive and low productivity

The experiment found that shallow dish culture was limited by the mass transfer of nutrients, and the yield was not high.

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