Liquid phase chip of quantum dot coding microspheres

Abstract

The invention relates to a newly designed liquid phase chip of quantum dot coding microspheres. The liquid phase chip of the quantum dot coding microspheres comprises macromolecular polymer microspheres, probe molecules and specific fluorescent protein labeled report molecules, wherein the surfaces of the macromolecular polymer microspheres are chemically modified, and a quantum dot nano material is embedded into the microspheres; and the probe molecules are coupled on the chemically modified microsphere surfaces. The liquid phase chip improves the coding property of the microspheres; an organic fluorescent dye is replaced by quantum dots, so the liquid phase chip has the advantages of stable fluorescence, strong fluorescence, difficult photo-bleaching and the like; the liquid phase chip also has the advantages of excitation bandwidth and narrow emission spectrum, so the stability, sensitivity and resolution are higher; meanwhile, the liquid phase chip simplifies an optical detection system, improves detection speed, reduces the cost of instruments, and is easier to operate.

Description

technical field [0001] The invention belongs to the technical field of molecular biology analysis and detection, and relates to a novel liquid phase chip, in particular to a newly designed liquid phase chip of quantum dot coded microspheres. Background technique [0002] With the rapid development of analytical and diagnostic technology, the liquid phase chip developed in the mid-1990s, known as the post-gene era, is a new type of nucleic acid and protein research platform. It is a combination of flow cytometry technology and traditional chips. A new technology platform combining technologies. The system organically integrates colored microspheres, modern immune technology, laser technology, applied fluidics, the latest high-speed digital signal processor and computer algorithms, resulting in extremely high detection specificity and sensitivity. It can be used for clinical disease diagnosis, such as detection of cytokines, allergens and autoimmune reactions, HLA typing, SNP...

AI Technical Summary

Problems solved by technology

In actual use, we found that Luminex liquid phase chips and similar chips have the following defects: (1) The fluorescent dyes used have some inherent shortcomings in optical characteristics, such as: narrow excitation band, wide emission spectrum, light instability, Easy photobleaching, etc., which limit its high throughput, high sensitivity and precise resolution in application; (2) The liquid chip system uses two organic fluorescent dyes to encode particles, and the characteristic of fluorescent dyes is the specificity of each dye Excitation wavelengths are different, even if the emission wavelength is the same, the excitation wavelength may be different

Therefore, the liquid chip system requires at least two excitation lights, resulting in a complex optical system, large volume, high cost, and also limits its detection speed

(3) The design of the micro sheath system through which a single microsphere passes restricts the detection speed

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