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Method for preparing powdered sodium hyaluronate

A sodium hyaluronate, powdery technology, applied in the field of preparation of sodium hyaluronate, can solve the problems of increasing the risk of bacterial contamination of sterile sodium hyaluronate raw materials, time-consuming dripping, relatively high requirements, etc., to reduce Risk of bacterial contamination with sodium hyaluronate, shortening of dropping time, and effects of reducing production costs

Active Publication Date: 2013-07-10
SHANGHAI JINGFENG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] 1 Since the amount of ethanol to be added dropwise is twice the volume of the sodium hyaluronate solution, the amount is relatively large, and the addition is very time-consuming, thereby greatly increasing the production cost
[0011] 2. When adding ethanol, it must be stirred continuously and with a certain strength. If the stirring is uneven or the ethanol drips on the wall or the stirring shaft, resulting in too high local ethanol concentration, it can lead to the formation of fibrous hyaluronic acid Sodium, so the requirements for production equipment and operation process are relatively high, which not only increases the cost, but also fails to obtain the ideal powdery sodium hyaluronate properties
[0012] 3. Due to the long time of adding ethanol, it will increase the risk of bacterial contamination of sterile sodium hyaluronate raw materials

Method used

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  • Method for preparing powdered sodium hyaluronate
  • Method for preparing powdered sodium hyaluronate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] In the final alcohol precipitation step of sodium hyaluronate prepared by the cockscomb extraction method, 50 grams of the intermediate sodium hyaluronate prepared in the previous process was added into 10 L of water, stirred and dissolved to obtain a sodium hyaluronate solution. Take 20L of 95% ethanol, add it into the sodium hyaluronate solution under stirring state and mix well. Take 100 grams of sodium chloride and add 300 milliliters of water to dissolve it, and slowly add it dropwise to the above mixed solution at a speed of 40 ml / min. During this process, constant stirring is required. After the powdered sodium hyaluronate is alcohol-precipitated, extract it into a filter washing and drying machine. After filtering the mother liquor, add about 10L of 95% ethanol to stir and dehydrate it for 5 minutes, and repeat this three times. After the dehydration is completed, the powder is sucked dry, and dried under a vacuum pressure of 0.08MPa and a temperature of about 4...

Embodiment 2

[0033] In the final alcohol precipitation step of the sodium hyaluronate purification production process prepared by bacterial fermentation, 50 grams of the sodium hyaluronate intermediate product prepared in the previous process was added to 10 L of water, stirred and dissolved to obtain a sodium hyaluronate solution. Take 20L of 95% ethanol, add it into the sodium hyaluronate solution under stirring state and mix well. Take 200 grams of sodium chloride and add 600 milliliters of water to dissolve it, then slowly add it dropwise to the above mixed solution at a speed of 50 ml / min, and continuous stirring is required during this process. After the powdered sodium hyaluronate is alcohol-precipitated, extract it into a filter washing and drying machine. After filtering the mother liquor, add about 10L of 95% ethanol to stir and dehydrate it for 5 minutes, and repeat this three times. After the dehydration is completed, the powder is sucked dry, and dried under a vacuum pressure ...

Embodiment 3

[0035] In the final alcohol precipitation step of sodium hyaluronate prepared by the cockscomb extraction method, 50 grams of the intermediate sodium hyaluronate prepared in the previous process was added into 10 L of water, stirred and dissolved to obtain a sodium hyaluronate solution. Take 20L of 95% ethanol, add it into the sodium hyaluronate solution under stirring state and mix well. After taking 300 grams of sodium chloride and adding 900 milliliters of water to dissolve, slowly add it dropwise to the above mixed solution at a speed of 60 ml / min, and continuous stirring is required during this process. After the powdered sodium hyaluronate is alcohol-precipitated, extract it into a filter washing and drying machine. After filtering the mother liquor, add about 10L of 95% ethanol to stir and dehydrate it for 5 minutes, and repeat this three times. After the dehydration is completed, the powder is sucked dry, and dried under a vacuum pressure of 0.08MPa and a temperature o...

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Abstract

The invention discloses a method for preparing powdered sodium hyaluronate. In a step of precipitating sodium hyaluronate in ethanol, the ethanol and solution of sodium hyaluronate are mixed uniformly first, then solution of sodium chloride is slowly dripped into the mixed solution with stirring, and thus, the powdered sodium hyaluronate is obtained. When the method is used for prepared powdered sodium hyaluronate, the preparation time can be shortened greatly, so the energy consumption in a production process is reduced and cost is reduced; and the microbiological contamination risks in the preparation of sterile sodium hyaluronate are reduced. The method is particularly suitable for industrial mass production.

Description

technical field [0001] The invention relates to a preparation method of sodium hyaluronate, in particular to a preparation method of powdery sodium hyaluronate. Background technique [0002] Hyaluronic acid (HA for short) is composed of (1→3)-2-acetylamino-2-deoxy-β-D-glucose-(1→4)-O-β-D-glucuronic acid A straight-chain polysaccharide composed of disaccharide repeating units, the molecular formula is (C14H21NO11)n, and the molecular weight varies from 2×10 depending on the source of the tissue 5 ~7×10 6 , the number of disaccharide units is 300 to 11,000 pairs. [0003] [0004] Commercial hyaluronic acid is generally in the form of sodium salt, white fibrous or powdery solid, has strong hygroscopicity, is soluble in water, and insoluble in organic solvents. The macromolecular network structure of sodium hyaluronate passes through the interaction with H 2 O forms hydrogen bonds to bind a large amount of water, and has the functions of forming various matrices, regulat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/08
Inventor 马贤鹏李建勇陈亮黄宇
Owner SHANGHAI JINGFENG PHARMA
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