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Brassica campestris aging-related transcription factor and coding gene and application thereof

A technology of head cabbage and transcription factors, which is applied in the fields of application, genetic engineering, plant gene improvement, etc., and can solve problems such as obvious limitations

Inactive Publication Date: 2011-04-13
FUDAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

While many traits have been greatly improved, their limitations are becoming increasingly apparent

Method used

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  • Brassica campestris aging-related transcription factor and coding gene and application thereof
  • Brassica campestris aging-related transcription factor and coding gene and application thereof
  • Brassica campestris aging-related transcription factor and coding gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1. Acquisition of genes encoding non-heading Chinese cabbage senescence-related transcription factors.

[0022] 1.1 RNA extraction

[0023] The non-heading Chinese cabbage variety "Xia Dongqing" was selected as the material, and about 0.1 g of yellowed leaf material was taken from in vitro storage. After the liquid nitrogen is fully ground, transfer to a 1.5ml centrifuge tube, add 1ml (Invitrogen Company), after mixing, place at room temperature for 15 minutes, add 0.2ml of chloroform:isoamyl alcohol (24:1), shake vigorously for 15 seconds, place at room temperature for 5 minutes, centrifuge at 13000rpm, 4°C for 15 minutes. Take the supernatant and add an equal volume of isopropanol, mix carefully, place at room temperature for 15 minutes, centrifuge at 13000 rpm, 4°C for 15 minutes. The precipitate was washed with 70% ethanol and dried at room temperature for 15 minutes. Dissolve in an appropriate amount of ddH2O water treated with 0.1% DEPC, and store at ...

Embodiment 2

[0028] Example 2: Functional analysis of non-heading Chinese cabbage senescence-related transcription factor BcNAC1.

[0029] 2.1 Sequence comparison analysis

[0030] The homology of BcNAC1 with other species was analyzed by Genedoc software. The analysis results showed that BcNAC1 not only had five subdomains conserved in the NAC family, but also the E subdomain was highly conserved. The GenBank accession numbers of Arabidopsis AtNAP, bean PvNAP and soybean GmNAP are: NP_564966.1, AAK84884.1, AAY46121.1, respectively.

[0031] In order to analyze the phylogenetic relationship between BcNAC1 and other senescence-associated transcription factors NAP, a phylogenetic tree of BcNAC1 and other NAPs was established with MEGA software. The results showed that BcNAC1 had the closest relationship with Arabidopsis AtNAP. Kidney bean, medicago, soybean, arabidopsis, nightshade, populus, wheat, rice, tomato The accession numbers of the NAP sequences of tomato), petunia and potato are...

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Abstract

The invention belongs to the technical field of plant genetic engineering, and particularly discloses a brassica campestris aging control-related neuro associative conditioning (NAC) family transcription factor and a coding gene and application thereof. The aging-related transcription factor provided by the invention is from 'Xia Dongqing' in brassica campestris varieties, is named BcNAC1, and has an amino acid sequence shown as SEQ ID NO.2. The coding gene of the brassica campestris aging-related transcription factor has a nucleotide sequence shown as SEQ ID NO. 1, or the coding gene is polynucleotide with the amino acid sequence shown as the SEQ ID NO.2. The gene can be used for studying a brassica campestris anti-aging molecular mechanism, improving the delaying of plant aging properties, delaying the aging of brassica campestris laminae, improving the nutritional quality loss of the picked laminae and the like.

Description

technical field [0001] The invention belongs to the technical field of plant genetic engineering, and specifically relates to an aging-related transcription factor, its coding gene and application, in particular to non-heading Chinese cabbage aging-related transcription factor, its coding gene and its application. Background technique [0002] Plant senescence refers to the process of gradual decline of an organ or the life function of the whole plant. It is a series of physiological decline processes before natural death, and is the result of long-term evolution and natural selection (Li Qing, Zhu Yuxian, Molecular Plant Breeding, No. 1 Vol. 3, 2003). The research on plant senescence mainly focuses on the study of leaf senescence. During leaf senescence, many changes occur in cell structure, physiological and biochemical metabolism, and gene expression regulation [Gepstein S, Genome Biology, 5(3):212,2004; Chandlee JM, Physiologia Plantarum, 113:1-8,2001 ; Nam HG, Trends ...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N5/10A01H5/00
Inventor 蒯本科邱凯魏强梁宁菁
Owner FUDAN UNIV
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