Lovastatin-generating fungus and application thereof in production of Pu'er tea

A technology of lovastatin and fungi, applied in the biological field, to achieve the effect of shortening the processing time

Active Publication Date: 2011-04-13
滇之坊实业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

How to control the content of lovastatin in Pu'er tea through the study of microorganisms in the processing of Yunnan Pu'er tea has not yet been published in the literature.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Embodiment 1: the separation and purification of purple Monascus purpurevs (Monascus purpurevs) bacterial classification

[0014] From the collected samples, pass through PDA medium (200g potatoes, peeled and cut into pieces, add 1000mL of water to boil for half an hour, filter with gauze, add 20g of glucose and 18g of agar, sterilize at 121°C for 30 minutes and then pour the plate) and malt Juice culture medium (wort juice 750-800ml, add water to 1000mL, add 15-18g agar, sterilize at 121°C for 30 minutes and pour plate) for separation and purification.

[0015] The separation and purification method is as follows:

[0016] (1) Melt the solid culture medium, cool it to 45°C, pour it into a sterile petri dish, about 15-20 ml per dish, shake it slightly, let it stand still, and make it into a plate for later use.

[0017] (2) Take 1 g of the pulverized sample to be separated and add it to a test tube with 9 ml of sterile water next to the flame. At this time, 10 -2 bact...

Embodiment 2

[0021] Embodiment 2: the screening of producing lovastatin purple Monascus (Monascus purpurevs)

[0022] (1) Liquid fermentation

[0023] Isolate and purify the obtained strains, inoculate wort slant, culture at 28°C for 6 days, wash with 10mL sterile water into a small Erlenmeyer flask with glass beads to make spore suspension. Inoculate 3mL of spore suspension into 50mL liquid seed medium (50ml per 250ml Erlenmeyer bottle) at 30°C, 150r / m, and cultivate for 2-3d until the color of the liquid is reddish.

[0024] Put the liquid seed culture medium into the liquid fermentation medium (250 mL liquid volume 50 mL) according to the inoculum amount of 5% at 28 ° C and 150 r / m for about 12 to 14 days.

[0025] (2) Preliminary screening by thin layer chromatography

[0026] Take 1 mL of fermentation broth, add 5 mL of ethyl acetate → shake at 150 r / min at 30 °C for 3 hours → centrifuge, collect the supernatant → extract the precipitate twice with 5 mL of ethyl acetate → combine th...

Embodiment 3

[0029] Embodiment 3: the application of purple Monascus purpurevs (Monascus purpurevs) in the fermentation of Pu'er tea

[0030] The maocha is moist-heated according to the traditional method, and then inoculate the maocha with a solid starter of 5% by weight of Monascus purpurevs (Monascus purpurevs) to ferment it, and turn the pile regularly. When the pile temperature rises to 25±3°C, Monascus purpurevs begin to metabolize. Lovastatin is a secondary metabolite of Monascus. It begins to be produced 3-5 days after inoculation and fermentation, and the production reaches the highest after 12-15 days.

[0031] The lovastatin content of the Pu-erh tea produced after inoculation with Monascus purpurevs reaches up to 0.263 mg / mL, and the tea has a strong and bright red soup color, a sweet and smooth taste, and a prominent aroma and ester aroma.

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Abstract

The invention relates to a lovastatin-generating fungus and the application thereof in the production of Pu'er tea, belonging to the biotechnology field. The production strain of the invention is obtained by separating, purifying and primarily filtering through thin layer chromatography and high efficiency liquid phase secondarily filtering. The production strain is lovastatin-generating Monascus purpurevs MPT13 with the preservation No. of CCTCC M 2010123, and is preserved on May 20, 2010, in the preservation department of China center for type culture collection located in Wuhan University, Wuhan. The lovastatin-generating fungus of the invention is applied to the production of Pu'er tea. The invention has the advantages that Monascus purpurevs is proportionally vaccinated and applied to the fermentation process of Pu'er tea to obtain the lovastatin-contained Pu'er tea with new characteristics in aspects of aroma and soup on a basis of keeping the flavor of traditional Pu'er tea, the processing time is shortened, and the invention plays an important role in the development of new Pu'er tea products and specific Pu'er tea.

Description

Technical field: [0001] The invention relates to a lovastatin-producing fungus and its application in the production of Pu'er tea, belonging to the field of biotechnology. Background technique: [0002] In recent years, Pu'er tea has attracted widespread attention due to its good lipid-lowering effect and unique quality, and many scholars reported that lovastatin was found in Pu'er tea. The statin drug is hydroxymethylglutaryl-CoA (HMG-CoA ) reductase inhibitors are the most classic and effective lipid-lowering drugs, widely used in the treatment of hyperlipidemia, but there is no systematic study on lovastatin and microorganisms in Puer tea, and no lovastatin Research on the application of statin strains in Pu-erh fermentation. [0003] The use of Monascus to produce red yeast rice and the production and application of red yeast pigment have a history of thousands of years, and its use has gradually expanded from the medical field to the food field. The secondary metaboli...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A23F3/10C12R1/645
Inventor 周红杰卓婧魏珍珍赵明李亚莉袁文侠李家华秘鸣
Owner 滇之坊实业有限公司
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