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Bacterial strain for degrading zearalenone toxin and application thereof

A zearalenone and strain technology, applied in the directions of application, bacteria, microorganism-based methods, etc., can solve problems such as zearalenone toxin pollution, achieve low production and use costs, ensure food safety, and use safety. Effect

Active Publication Date: 2011-04-13
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The object of the present invention is to develop and develop bacterial strains capable of degrading zearalenone and its application in view of the extensive pollution of zearalenone toxins in grain production and feed processing and storage.

Method used

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  • Bacterial strain for degrading zearalenone toxin and application thereof
  • Bacterial strain for degrading zearalenone toxin and application thereof
  • Bacterial strain for degrading zearalenone toxin and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Obtaining of Bacillus sp.ZDS-1

[0027] Soil samples were collected from the corn field of Jiangsu Academy of Agricultural Sciences, and zearalenone toxin was used for multiple subculture enrichment cultures in the laboratory (30°C, once a week) to obtain the degradable zearalenone toxin The bacterial suspension was spread and cultured on LB medium (30°C, 3 days), and different colonies were picked on the plate to verify the degradation effect on zearalenone toxin one by one, and finally Obtain a strain capable of degrading zearalenone toxin, and define it as Bacillus sp.ZDS-1, add glycerol, and store it in a -70°C refrigerator.

[0028]By measuring the physiological and biochemical characteristics of Bacillus sp.ZDS-1, cloning the 16SrDNA sequence of Bacillus sp.ZDS-1 and sequencing the 16SrDNA sequence, and comparing the sequencing results with BLAST in Genbank, the system of Bacillus sp.ZDS-1 was determined developmental evolutionary status. Through the identificat...

Embodiment 2

[0031] Preparation of liquid inoculum

[0032] The seed medium (nutrient broth medium) is: peptone 0.5%, beef extract 3%, NaCl 0.5%, and the pH of the seed medium is 7.0-7.2.

[0033] The fermentation medium is: glucose 1.0%, (NH 4 ) 2 SO 4 0.1%, K 2 HPO 4 0.2%, MgSO 4 0.02%, NaCl 0.01%, CaCO 3 0.3%, peptone 0.05%, appropriate amount of distilled water; the pH of the fermentation medium is 7.2-7.5.

[0034] The ZDS-1 strain was activated on the seed medium, and the degradation performance was measured, and inoculated on the inclined surface of the test tube to obtain a test tube seed for future use.

[0035] Inoculate the test tube species into a 1000mL shake flask containing 200mL of seed medium, and culture it with constant temperature shaking to the logarithmic phase to obtain the strain.

[0036] A 500-liter seed tank is used, and the feeding amount of the seed medium is 400 liters. After the feeding is completed, it is sterilized by high-pressure damp heat at ...

Embodiment 3

[0039] Preparation of solid bacterial agent 1

[0040] The ZDS-1 liquid bacterial agent produced in Example 2 and the collected sieved and dried peat were mixed evenly in a weight ratio of 1:4 to form a solid, thereby making the ZDS-1 solid microbial agent.

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Abstract

The invention relates to a bacterial strain for degrading zearalenone toxin, which is characterized in that: the preserving number of the bacterial strain is CCTCC NO: M2010053; and the Genbank accession number of the bacterial strain 16S rDNA is HM372880. When in application, the bacterial strain is prepared into liquid bactericide or solid bactericide which can degrade the zearalenone toxin for degrading the zearalenone toxin in grains or fodders. The bacterial strain has the advantages that: the process of using the bacterial strain to degrade the zearalenone toxin is free of secondary pollution, thereby improving the quality of agricultural products and ensuring the edible safety of people and livestock; and the bacterial strain is used for producing various preparations which are used for degrading the zearalenone toxin the bacterial strain, which is not only low in production and using cost, but also safe in use.

Description

technical field [0001] The invention relates to a bacterial strain capable of degrading zearalenone and its application, in particular to a bacterial strain capable of degrading zearalenone toxin and its application. Background technique [0002] Zearalenone (Zearalenone, ZEA) is an estrogen-like mycotoxin produced by Fusarium spp. Acid lactones are widely found in moldy corn, sorghum, wheat, oats, barley and other cereal crops and dairy products. The most common genus of zearalenone-producing bacteria is Fusarium graminearum, and Fusarium tritina, Fusarium equiseti, Fusarium nivalorum, Fusarium pink, etc. Studies have shown that zearalenone has strong reproductive toxicity, which can reduce the embryo survival rate of pregnant animals and the birth weight of newborn fetuses, resulting in a decline in animal production performance; moreover, the toxin is strongly toxic to the liver and liver cells It has immunosuppressive effect and promotes the occurrence and development ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L1/015C12R1/07A23L5/20
Inventor 徐剑宏史建荣王宏杰祭芳林凡云吴季荣
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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