Enhancing method of production strength of 2-keto-L-gulonic acid
A technology for producing high-strength and gulonic acid, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem of not being able to use L-sorbose, and achieve the effect of reducing costs
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Embodiment 1
[0047] At 1 m 3 In the fermenter, the initial L-sorbose concentration of the fermentation medium is 16.15 g·L -1 After 6 hours of fermentation, start to add L-sorbose (ie aseptic one-step fermentation broth), and control the concentration of L-sorbose to 15~30 g·L during the feeding process -1 After 39 hours of fermentation, the addition of L-sorbose is finished, the dissolved oxygen in the fermentation process is controlled at 40%, and the concentration of 2-KLG at the end of the fermentation is 77.09 g·L -1 , The fermentation period is 51 h, the acid production rate is 1.51 g·(L·h) -1 ( figure 1 ).
Embodiment 2
[0049] At 1 m 3 In the fermenter, the initial L-sorbose concentration of the fermentation medium is 10.15 g·L -1 , Add 0.21 g·L -1 L-glycine and 0.24 g·L -1 L-Proline, fermented for 4 hours, start to add L-sorbose (that is, aseptic one-step fermentation broth), control the concentration of L-sorbose to 15~30 g·L during the feeding process -1 After 29 hours of fermentation, the addition of L-sorbose ends, the dissolved oxygen in the fermentation process is controlled at 40%, and the 2-KLG concentration at the end of the fermentation is 80.29 g·L -1 , The fermentation period is 40 h, the acid production rate is 2.00 g·(L·h) -1 ( figure 2 ).
Embodiment 3
[0051] At 1 m 3 In the fermenter, the initial L-sorbose concentration of the fermentation medium is 10.15 g·L -1 , Add 0.1 g·L -1 L-glycine and 0.8 g·L -1 L-Proline, fermented for 4 hours, start to add L-sorbose (that is, aseptic one-step fermentation broth), control the concentration of L-sorbose to 15~30 g·L during the feeding process -1 After 29 hours of fermentation, the addition of L-sorbose ends, the dissolved oxygen in the fermentation process is controlled at 40%, and the 2-KLG concentration at the end of the fermentation is 78.34 g·L -1 , Fermentation period 43 h, acid production rate 1.88 g·(L·h) -1 .
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