Method for extracting refined tea saponin from camellia oleifera seed cake

A technology for tea saponin and camellia seed cake, which is applied in chemical instruments and methods, preparation of sugar derivatives, steroids, etc., can solve the defects of process, purity, yield or cost, restrict the application of tea saponin, and cause eye damage. major problems, to achieve the effect of low cost, low production cost and good physical and chemical stability

Inactive Publication Date: 2011-04-20
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the experimental procedure is simple, the reagent methanol used is highly toxic, especially harmful to the eyes, and is not suitable for use in industrial production, and the purity of tea saponin obtained by this method is only 85.36%.
[0006

Method used

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  • Method for extracting refined tea saponin from camellia oleifera seed cake
  • Method for extracting refined tea saponin from camellia oleifera seed cake
  • Method for extracting refined tea saponin from camellia oleifera seed cake

Examples

Experimental program
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Effect test

Embodiment 1

[0036] Weigh 190 g of pulverized camellia seed cake, extract with 950 mL of 85% ethanol under reflux, and extract continuously for 2 times, each time for 3 hours. The extracts were combined, concentrated under reduced pressure, ethanol was recovered, and the concentrated solution was diluted with water to a ratio of solid to liquid of 1:5. In a water bath at 50°C, add 55 mL of chitosan solution, stir thoroughly, and after standing for 3 hours, filter the solution with suction to remove the precipitate to obtain a transparent and clear liquid. Weigh 213g of pretreated AB~8 macroporous adsorption resin and put it into a glass column. The transparent and clear liquid is first fully absorbed by AB~8 macroporous adsorption resin, and then the impurities are eluted with 0.05% sodium hydroxide solution. Wash with ion water until the effluent is neutral. Then elute with 70% ethanol until no tea saponin is detected in the effluent, and this part of the eluate is collected. Add hydrog...

Embodiment 2

[0038] Weigh 200 g of pulverized Camellia oleifera seed cake, extract with 1200 mL of 85% ethanol under reflux, and extract continuously for 2 times, each time for 2.5 hours. The extracts were combined, concentrated under reduced pressure, ethanol was recovered, and the concentrated solution was diluted with water to a ratio of solid to liquid of 1:6. In a water bath at 60°C, add 55mL of chitosan solution, stir well, let stand for 2 hours, filter the solution with suction, remove the precipitate, and obtain a transparent and clear liquid. Weigh 214g of pretreated AB~8 macroporous adsorption resin and put it into a glass column. The transparent and clear liquid is first fully absorbed by AB~8 macroporous adsorption resin, and then the impurities are eluted with 0.2% sodium hydroxide solution. Wash with ion water until the effluent is neutral. Then elute with 70% ethanol until no tea saponin is detected in the effluent, and this part of the eluate is collected. Add hydrogen pe...

Embodiment 3

[0040] Weigh 200 g of pulverized Camellia oleifera seed cake, extract with 800 mL of 85% ethanol under reflux, and extract continuously for 3 times, each time for 2 hours. The extracts were combined, concentrated under reduced pressure, ethanol was recovered, and the concentrated solution was diluted with water to a ratio of solid to liquid of 1:5. In a water bath at 50°C, 60 mL of chitosan solution was added, fully stirred, and allowed to stand for 2.5 hours, and the solution was suction-filtered to remove precipitates to obtain a transparent and clear liquid. Weigh 213g of pretreated AB~8 macroporous adsorption resin and put it into a glass column. The transparent and clear liquid is first fully absorbed by AB~8 macroporous adsorption resin, and then the impurities are eluted with 0.05% sodium hydroxide solution. Wash with ion water until the effluent is neutral. Then elute with 80% ethanol until no tea saponin is detected in the effluent, and this part of the eluate is col...

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Abstract

The invention provides a method for extracting refined high-purity tea saponin by taking camellia oleifera seed cake as raw material. The method comprises the following steps: smashing the camellia oleifera seed cake after squeezing and oil extraction; carrying out reflux extraction with 70-95% ethanol; adding chitosan (the volume ratio of the chitosan solution to extraction liquid is 9-13:100) to the extraction liquid for flocculation and purification; eluting and removing impurities from the purified clear liquid with a sodium hydroxide solution after the purified clear liquid is absorbed by AB-8 macropore adsorbent resin; eluting the tea saponin with 60-90% ethanol; decoloring the eluent containing the tea saponin with hydrogen peroxide (the volume ratio of the hydrogen peroxide to the eluent is 3-6:100); and steam drying the decolored solution to obtain milky powdery tea saponin. The invention has the advantages of simple process, no pollution and low production cost, the purity is higher than 95%, and the tea saponin has good color and can be widely applied to the fields of daily chemical industry, textile, medicine, food industry and the like.

Description

technical field [0001] The invention belongs to the field of deep processing of agricultural and forest by-products, and relates to a method for preparing tea saponin, in particular to a method for extracting and refining tea saponin from oil-pressed camellia seed cake. Background technique [0002] Tea saponin belongs to the pentacyclic tricyclic saponins, and its molecules are composed of hydrophilic sugar bodies, hydrophobic ligands and organic acids. Because of its unique physical and chemical properties, tea saponin is widely used as an additive in daily chemicals and food. For example, tea saponin has a variety of good surface activities such as foaming, foam stabilization, emulsification, and dispersion. The detergent prepared with it is less affected by water hardness, and has a great effect on washing wool, silk, down, etc. Superiority; Pharmacological studies have shown that tea saponin has significant pharmacological functions such as antibacterial and anti-infla...

Claims

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Application Information

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IPC IPC(8): C07J63/00C07H15/256C07H1/08
Inventor 李海玲邓清清韩定献高中洪
Owner HUAZHONG UNIV OF SCI & TECH
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