Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Hydroquinone compound with anti-HCV (Hepatitis C Virus) activity and preparation method thereof

A hydroquinone and compound technology, applied in the field of hydroquinone compounds and their preparation, and new compounds, can solve the problems that the anti-HCV activity of compound euchroquinolA has not been reported yet, and achieve simple separation methods, novel structures, high efficiency effect

Inactive Publication Date: 2011-05-04
KUNMING UNIV OF SCI & TECH
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] So far, the structure and anti-HCV activity of the compound euchroquinol A involved in the present invention have not been reported

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Hydroquinone compound with anti-HCV (Hepatitis C Virus) activity and preparation method thereof
  • Hydroquinone compound with anti-HCV (Hepatitis C Virus) activity and preparation method thereof
  • Hydroquinone compound with anti-HCV (Hepatitis C Virus) activity and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A. Dried 20 kg Xinjiang comfrey ( A. euchroma ) After the stem bark is crushed, according to the mass / volume ratio (kg / L) of comfrey powder: ethanol aqueous solution = 1:2, 40 L of ethanol aqueous solution with a volume concentration of 95% is fully mixed with 20 kg of Xinjiang comfrey powder, Soak at room temperature for 24 hours to obtain an extract, repeat the extraction for 3 times, and combine the extracts;

[0025] B. Concentrate the extract in step A under reduced pressure as usual, recover ethanol until there is no ethanol smell, then use petroleum ether to extract repeatedly at room temperature according to the volume ratio of extract:petroleum ether=1:1 Second, after recovering the petroleum ether, the extract was obtained;

[0026] C. According to the volume ratio of extract: ethyl acetate=1:1, the extract of step B was repeatedly extracted 3 times with ethyl acetate at room temperature, and concentrated under reduced pressure to recover ethyl acetate to ob...

Embodiment 2

[0036] In this example, Huh 7.5.1 cells were infected with type 2a chimeric virus J6 / JFH1 to produce infectious virus particles, an HCV cell culture system (HCV cell culture, HCVcc), with interferon and ribavirin as positive controls , MTT cytotoxicity test and in vitro anti-HCV efficacy evaluation of anti-HCV drugs.

[0037] The hydroquinone compound euchroquinol A was dissolved in DMSO, 0.22 mu M membrane filter sterilization, the concentration of the original drug solution was 10 mg / mL.

[0038] Human liver cancer cell line Huh 7.5.1 was treated with DMEM (Dulbecco’s modified Eagle’s medium, DMEM) containing 15% newborn bovine serum at 37°C and 5% CO 2 cultured in an incubator. Virus J6 / JFH1 was cultured from Huh 1 cells and stored at -80°C.

[0039] 1) MTT method to detect the cytotoxicity of samples

[0040] Huh 7.5.1 cells in the logarithmic growth phase were taken, and 9×10 3 cells / well cells were spread in 96-well plate, after 5 hours of attachment, add 2 mu L...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a hydroquinone compound which has anti-HCV (Hepatitis C Virus) activity, a benzo-geijerene framework and the selection index of 6.32 and can be used as a lead compound for treating HCV. The hydroquinone compound is extracted and separated from stem barks of Arnebiaeuchroma; and the hydroquinone compound has the advantages of simple and easiness of extraction and separation methods, low cost, high efficiency and yield and good extract quality. A molecular formula of the hydroquinone compound with the anti-HCV activity is C16H18O3, and a chemical structure is shown as a specification.

Description

technical field [0001] The invention relates to a new compound, especially a hydroquinone compound with anti-HCV activity and a preparation method thereof, belonging to the technical field of medicines. Background technique [0002] Hepatitis C virus (HCV), the leading cause of liver cancer and cirrhosis, infects more than 3% of the world's population and is considered one of the leading causes of chronic liver disease. The current standard treatment, including PEG-IFN / RBV combination, is only effective for more than half of the patients infected with hepatitis C virus (HCV), and has significant side effects. The treatment effect is not ideal. Therefore, it is of great practical significance to search for new anti-HCV drugs or lead compounds from traditional Chinese medicine, plants and fungi resources. However, the screening platform for anti-HCV compounds is not yet perfect compared with other viral screening platforms. There are few reports about anti-HCV natural produ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07D307/92A61P31/14A61P1/16
Inventor 李蓉涛李洪梅李海舟夏雪山李晓蕾
Owner KUNMING UNIV OF SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com