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Bacillus mucilaginosus with potassium-decomposing function and application thereof

A technology for bacillus colloid and potassium solution, applied in the application, bacteria, organic fertilizer and other directions, can solve the problems of ecological environment damage, low utilization rate, high cost, improve the quality of agricultural products, improve the soil microenvironment, and reduce the application amount Effect

Inactive Publication Date: 2011-05-18
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Potassium fertilizer resources are scarce in China, and a large amount of imports is needed to meet the development of modern agriculture. The chemical potash fertilizers commonly used at present not only have high cost, low utilization rate, but also cause certain damage to the ecological environment and affect the quality of agricultural products.

Method used

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  • Bacillus mucilaginosus with potassium-decomposing function and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1. Isolation and Identification of Bacillus mucilaginosus NK 102

[0021] The strain was isolated from farmland soil in the suburbs of Jining City, Shandong Province.

[0022] The specific implementation steps are as follows: under aseptic operation, weigh 1.0 g of soil sample and dissolve it in 9 ml of sterile physiological saline, shake it fully, dilute it in gradient, and spread it on the prepared separation medium plate. Put it upside down in an incubator at 28-30°C and cultivate it for 2-3 days, use an inoculation needle to pick up the colonies with rounded edges, moist, viscous, colorless, transparent and elastic, and further separate and purify. Then the strains were inoculated in the potassium solution assay medium, and re-screened to determine the silicate strains. After Gram staining and 16S rDNA gene sequence analysis, it was determined that the strain was Bacillus mucilaginosus NK 102, and the strain was named Bacillus mucilaginosus NK 102, and the ...

Embodiment 2

[0023] Embodiment 2, the preparation of potassium ion standard curve

[0024] Potassium standard solution preparation. Take 1000ppm K single-element standard solution mother solution, respectively accurately dilute to 0, 0.1, 0.2, 0.3, 0.4ppm K standard solution, measure by atomic absorption spectrophotometry, and draw the working curve. As attached to the manual figure 1 shown.

Embodiment 3

[0025] Embodiment 3, Bacillus mucilaginosus (Bacillus mucilaginosus) NK 102 potassium solution assay experiment 1

[0026] The potassium-dissolving medium is used, and the specific implementation steps are as follows: the strains are connected to the potassium bacteria liquid activation medium, cultured with shaking at 30°C for 24 hours, inoculated with 2% inoculum in the potassium-dissolving medium, and the control group is inserted into the same amount of inactivation Bacterial liquid, the liquid volume is 50ml, three parallels for each group, 24°C, pH 6.5, 160rpm shaking culture for 10 days; take 5ml of fermentation liquid, put it in boiling water at 100°C for digestion, and add 6% HO intermittently 2 o 2 Until the fermented liquid is clarified, filter, settle to 100ml, and then dilute 5 times. The content of potassium ion is determined by atomic absorption spectrophotometry. The water-soluble potassium in the experimental group is 47.35% higher than that in the control gro...

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Abstract

The invention discloses a bacillus mucilaginosus with a potassium-decomposing function and application thereof, and relates to a bacillus mucilaginosus and a method for decomposing silicate mineral to release effective potassium by using the bacillus mucilaginosus. The method comprises the steps of: inoculating a strain to a potassium bacterium liquid activating culture medium, shaking and culturing for 24h at a temperature of 30 DEG C, inoculating to a potassium decomposing measuring culture medium in an inoculating amount of 2-10 percent, inoculating a contrast with equivalent inactivating bacterium liquid, shaking and culturing for 10 days under the conditions that the liquid loading capacity is 50ml, the temperature is 24-37 DEG C, the pH value is 6.5-8.0 and the speed is 160rpm; and taking 5ml of fermenting liquid, placing in 100 DEG C boiled water for heating, intermittently adding 6 percent H2O2 until the fermenting liquid is clear, filtering, fixing the volume to 100ml, diluting by 5 times based on the fixed volume, and measuring the content of potassium ions by using an atomic absorption spectrophotometry. The strain can be applied to preparing a silicate bacterial fertilizer and decomposing aluminosilicate minerals of potash feldspar or mica and the like in soil so as to release soluble potassium to be absorbed and utilized by plants, thereby reducing the application amount of a chemical potassium fertilizer, improving the soil microenvironment, and improving the quality of agricultural products.

Description

technical field [0001] The invention relates to a new colloid bacillus strain and its application, in particular to a colloid bacillus (Bacillus mucilaginosus) NK 102 with potassium-dissolving function and a method for releasing silicate mineral potassium by using the colloid bacillus strain. Background technique [0002] Soil is a natural potassium pool, rich in potassium, but more than 95% of potassium exists in silicate minerals such as potassium feldspar or mica in a slow-acting state, which cannot be directly absorbed and utilized by crops, which leads to The soil is both rich in potassium and deficient in potassium. [0003] Potassium fertilizer resources are scarce in China, and a large amount of imports is needed to meet the development of modern agriculture. The chemical potash fertilizers commonly used at present not only have high cost and low utilization rate, but also cause certain damage to the ecological environment and affect the quality of agricultural produ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F11/08C09K17/00C12R1/07
Inventor 宋存江李保宾王淑芳李强郭文斌王媛媛解慧冯俊
Owner NANKAI UNIV
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