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Ginsenoside Rb3 composition and application thereof in preparing medicament for treating diabetes

A kind of technology of ginsenoside and composition, applied in the application field of treating diabetes

Inactive Publication Date: 2011-06-15
DALIAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The role of ginsenoside Rb3 in anti-diabetes and weight loss in vivo has not been reported yet

Method used

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  • Ginsenoside Rb3 composition and application thereof in preparing medicament for treating diabetes
  • Ginsenoside Rb3 composition and application thereof in preparing medicament for treating diabetes
  • Ginsenoside Rb3 composition and application thereof in preparing medicament for treating diabetes

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0018] Experimental Example 1 The hypoglycemic effect of ginsenoside Rb3 on alloxan-induced diabetic mice:

[0019] 1. Experimental materials, animals, instruments and reagents

[0020] 1.1 Experimental materials

[0021] American ginseng (or ginseng, Panax notoginseng) stem and leaf crude extract, after being dissolved in water, it is separated with NKA-12 macroporous resin, ethanol and water gradient elution, and panaxatriol group saponins and panaxadiol group saponins, panaxadiol group saponins are obtained The pure ginsenoside Rb3 (purity 98%) was obtained by repeated chromatography on a silica gel column (chloroform-methanol-water, n-butanol-ethyl acetate-water system).

[0022] The animal is a Kunming mouse, weighing 20-22 g, female. purchased from the Experimental Animal Center of Jilin University School of Basic Medicine.

[0023] 1.2 Instruments

[0024] Wenhao Beiyi type blood glucose meter and blood glucose test strips, Johnson & Johnson (China) Medical Devices ...

experiment example 2

[0052] Experimental Example 2 Effect of Ginsenoside Rb3 on Obese Mice Induced by High Fat Diet

[0053] 1 Experimental materials, instruments and reagents

[0054] 1.1 Materials

[0055] Ginsenoside Rb3 was prepared by this experimental group as described in item 1.1 of Example 1, and the content of Rb3 was 20%.

[0056] 1.2 Instruments and reagents

[0057] The total cholesterol kit was purchased from Zhongsheng Beikong Biotechnology Co., Ltd.; BS-400 automatic biochemical analyzer, Shenzhen Mindray Biomedical Electronics Co., Ltd.; BP211D electronic balance (1 / 100,000), Germany Sartorius company; Haobeiyi blood glucose meter and blood glucose test strips, Johnson & Johnson (China) Medical Devices Co., Ltd.

[0058] 1.3 Experimental animals

[0059] The animal is a Kunming mouse, female, purchased from the Experimental Animal Center of the Basic Medical College of Jilin University, weighing 19-22 g, and aged 3 weeks.

[0060] 1.4 Animal Feed

[0061] The conventional fe...

Embodiment 1

[0081] Embodiment 1: the extraction of ginsenoside Rb3

[0082] American ginseng (ginseng, panax notoginseng) stem and leaf crude extract 200g, after water-soluble, use NKA-12 macroporous resin to separate, 30%, 40%, 50% ethanol gradient elution, obtain saponins of panaxatriol group and panaxadiol group saponins. The obtained ginsenosides of the ginsenoside group were repeatedly chromatographed on a silica gel column (chloroform-methanol-water, n-butanol-ethyl acetate-water system) to obtain 10.5 g of pure ginsenoside Rb3.

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Abstract

The invention relates to a ginsenoside Rb3 composition and an application thereof in preparing a medicament for treating diabetes. The ginsenoside Rb3 composition is prepared from ginsenoside Rb3 as a main component and 1-5 of a carrier, an excipient, a sweetening agent, an adhesive, a flavoring agent, a preservative, a dispersing agent, a surfactant and a colorant as adjuvants, and the medicament is prepared into tablets, pills, granules, capsules and powder. The method for extracting the ginsenoside Rb3 comprises the following steps: dissolving crude extracts of stems and leaves of American ginseng, ginseng or pseudo-ginseng in water, then separating by NKA-12 macroporous resin, and performing gradient elution by ethanol water to obtain panaxatriol saponin and panaxadiol saponin. Repeated chromatography is preformed on the panaxadiol saponin by silicagel columns to obtain pure ginsenoside Rb3 with the purity of 98%. Proven by animal experiments, the ginsenoside Rb3 has notable effects of symptom improvement and treatment on diabetes and adiposis.

Description

technical field [0001] The invention belongs to the field of pharmaceutical preparations, and relates to the application of an extract derived from plant ginseng as medicine for treating diabetes. Background technique [0002] The research on the chemical constituents of ginseng has a history of more than one hundred years. The chemical composition of ginseng is relatively complex, including saponins, sugars, proteins, polypeptides, amino acids, organic acids, vitamins, volatile oils and other components. Among them, ginsenoside is the main active ingredient of ginseng. Many domestic and foreign scholars have studied the effect of ginseng on anti-diabetes and weight loss, including ginseng, ginseng extract, panaxadiol group and triol group saponins, and monomeric ginsenosides. The antidiabetic effect of ginsenoside Rb3 and the weight loss effect in vivo have not been reported yet. Contents of the invention [0003] The object of the present invention is to provide a gin...

Claims

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Application Information

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IPC IPC(8): A61P3/04A61P3/10A61K31/704A61P5/50
Inventor 弓晓杰郑毅男张成中卜其涛刘文丛刘发贵
Owner DALIAN UNIV
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