Glossy ganoderma extract and pharmaceutical application thereof
The technology of Ganoderma lucidum extract and Ganoderma lucidum spores is applied in the field of Ganoderma lucidum extract and its pharmaceutical use, and can solve the problems of reducing the physiological activity of Ganoderma lucidum spore oil and preparations thereof, oxidative deterioration of Ganoderma lucidum spore oil by heating, unfavorable for the development and utilization of Ganoderma lucidum spore oil, etc. Achieve the effect of not easy to deteriorate, avoid oxidative damage, and low toxicity
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Embodiment 1
[0061] Preparation of Ganoderma lucidum spore wall-breaking powder:
[0062] Take 100g of Ganoderma lucidum spore powder, stir well in 1000ml of 10% ethanol-water mixed solvent, soak for 3 hours, make the spores absorb water and swell, soften the external tissue, add 8000 IU / g pectinase, 5000 IU / g cellulase , 1% Tween-80, enzymolysis temperature 35°C, pH 7.5, enzymolysis time 3 hours, centrifuged, filtered, and freeze-dried to obtain Ganoderma lucidum spore-broken wall powder.
[0063] Freeze-dried spore powder becomes agglomerated and brown, and is set aside.
Embodiment 2
[0065] Detection of Ganoderma lucidum spore broken wall powder:
[0066] in area lmm 2 , A counting disc with a depth of 1.10mm. A large square in the center is divided into 25 middle squares with double lines, and each middle square is divided into 16 small squares for counting purposes.
[0067] counting method
[0068] Use an electro-optical analytical balance (accuracy 0.01 mg) to weigh 1 mg of Ganoderma lucidum broken spore powder into a 5 mL test tube, add 1 mL of distilled water to the test tube, and shake for about 10 minutes to dissolve the broken Ganoderma lucidum spore powder in the water evenly to obtain Ganoderma lucidum spores of the suspension.
[0069] Use a micro-syringe (5 μL) to absorb 2 μL of the suspension, drop the suspension into the counting disk, cover it with a glass slide, and let it stand for 5 minutes. After the suspension is evenly distributed in the counting disk, count.
[0070] Under the light microscope, the spore wall is broken or not. Th...
Embodiment 3
[0077] The extraction method of the fat-soluble components of Ganoderma lucidum spores adopts ethanol-water as the extraction solvent:
[0078] Take 100g of Ganoderma lucidum broken spore powder, add 0.5% Tween-80 to 1000ml of ethanol-water mixed solvent with a volume ratio of 4:6, stir well for 2 hours, centrifuge, filter, and recover ethanol from the filtrate, then separate out Ganoderma lucidum from water The fat-soluble components of spores, Tween-80 is mostly dissolved in water, only a very small amount of Tween-80 dissolves in the fat-soluble components of Ganoderma lucidum spores, and the remaining Tween-80 in the fat-soluble components of Ganoderma lucidum spores is washed with water to obtain Ganoderma lucidum Spore-soluble component.
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