The invention relates to a corn
straw pretreatment method with
synergy of composite fungi and stepped
temperature control, and belongs to the technical field of
agricultural waste resource utilization. In order to solve the problems of unbalanced
enzyme activity, strain competition and high
inoculation cost during corn
straw pretreatment by using composite fungi, the invention provides a corn
straw pretreatment method based on composite fungi synergistic stepped
temperature control, which comprises the following steps of: crushing and sieving corn straws, and adding a
fermentation culture medium to adjust the
moisture content to obtain corn straw
fermentation liquor; the method comprises the following steps: inoculating a composite fungal
spore solution into a corn straw
fermentation solution, and culturing for 15 days by adopting step
temperature control to finish pretreatment; the enzymatic activities of different fungi are accurately activated through staged temperature control, the degradation efficiency of the corn straw is improved through the synergistic effect, and the
cellulose degradation rate is improved by 21.8% compared with that of constant-temperature culture; and the
lignin degradation rate is improved by 36.0%. The stepped temperature control ensures that each
enzyme works at the optimal temperature, the
catalytic efficiency is maximized, the enzymolysis rate is improved, the pretreatment time is shortened, and the treatment cost is reduced.