A fully-automatic production line of manufacturing chopsticks by using plant branches is composed of a cutting machine, a burr removal machine, a cleaning machine, a drying, straightening and sterilizing machine, a separation device, an outer circle processing machine and a heat transfer machine, wherein the machines are connected with one another through conveying belts. According to the main processes, materials are conveyed into the cutting machine to be cut, and then sent to the burr removal machine, burr removal is carried out on the materials, and then the materials are conveyed into the cleaning machine through the conveying belts to be cleaned; microwave drying, straightening and sterilization are carried out on the materials conveyed into the drying, straightening and sterilizing machine; the materials are conveyed into the separation device through the conveying belts, the raw materials which can be processed are selected out rapidly, the matching raw materials automatically enter the outer circle processing machine after being screened out, and polishing is carried out on the outer circles; finally, the materials enter the heat transfer machine, and heat transfer printing and disinfection are carried out. The fully-automatic production line has the advantages of being a substitute of industrially producing the disposable wood chopsticks at high speed and with low cost, achieving good quality, low carbon, environment-friendliness, and hygienic security, conforming to industrial policies of the nation, and having wide market prospect.