Low-cost extraction method for yeast lipid

An extraction method and low-cost technology, applied in the biological field, can solve the problems of cumbersome process, low oil extraction rate, and high cost, and achieve the effects of large sample processing volume, improved extraction efficiency, and low cost

Inactive Publication Date: 2016-05-04
JINAN DEV ZONE XINGHUO SCI & TECH RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention provides a low-cost method for extracting yeast oil, aiming at the problems of cumbersome technology, high cost, and low oil extraction rate in the existing oil-producing yeast cell wall breaking technology

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Select Lipozyme Starrii, after fermenting and cultivating to a stable stage, concentrate to obtain a yeast suspension containing 30wt% of yeast sludge, add 0.1% sodium dodecylbenzenesulfonate, 0.2% shell Polysaccharide-acetic acid solution, 0.1% 5'-phosphodiesterase crude enzyme solution, pH value adjusted to 3.0, stirred at 30° C. for 10 h to obtain a yeast dissolution solution. Add the extraction solvent hexane to the yeast enzymolysis solution at a volume ratio of 1:2, control the ultrasonic power to 100W, and the frequency to 20KHz. After ultrasonic oscillation for 5 minutes, centrifuge at 3000rpm for 5 minutes to collect the upper mixed oil. After the mixed oil is distilled, the yeast oil is obtained, and the extraction rate is 90.6%.

Embodiment 2

[0020] Select Lipozyme Starrii, after fermenting and cultivating to a stable stage, concentrate to obtain a yeast suspension containing 50wt% of yeast sludge, add 1% sodium dodecylbenzenesulfonate, 2% shell Polysaccharide-acetic acid solution, 0.5% 5'-phosphodiesterase crude enzyme solution, pH value adjusted to 7.5, stirred at 50° C. for 20 hours to obtain a yeast dissolution solution. Add the extraction solvent to the yeast enzymatic hydrolysis solution at a volume ratio of 1:5, mix equal volumes of hexane and ethanol, control the ultrasonic power to 300W, and the frequency to 40KHz. After ultrasonic oscillation for 15 minutes, centrifuge at 5000rpm for 10 minutes to collect the mixed oil in the upper layer. After the mixed oil is distilled, the yeast oil is obtained, and the extraction rate is 96.3%.

Embodiment 3

[0022] Select Lipozyme Starrii, after fermenting and cultivating to a stable stage, concentrate to obtain a yeast suspension containing 40wt% of yeast sludge, add 0.5% sodium dodecylbenzenesulfonate, 1% shell Polysaccharide-acetic acid solution, 0.3% 5'-phosphodiesterase crude enzyme solution, pH value adjusted to 5.0, stirred at 40° C. for 10 h to obtain a yeast dissolution solution. Add the extraction solvent to the yeast enzymolysis solution according to the volume ratio of 1:3, mix the hexane and petroleum ether in equal volumes, control the ultrasonic power to 200W, and the frequency to 30KHz. After the ultrasonic oscillation is completed for 10 minutes, centrifuge at 4000rpm for 8 minutes to collect the upper layer of mixed oil. . After the mixed oil is distilled, the yeast oil is obtained, and the extraction rate is 94.1%.

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Abstract

The invention discloses a low-cost extraction method for yeast lipid. According to the method, cell wall breaking is accelerated in a yeast cell autolysis and external dissolution combined mode, the ultrasonic technology is utilized for improving the effect of an extraction agent, separation of lipid and cell components is realized, and the purpose of increasing the lipid extraction rate is achieved. The method has a good extraction effect on different kinds of yeast cell lipid, the one-time lipid extraction rate reaches 90% or above, operation is simple, special instruments and equipment are not needed, the sample processing quantity is large, cost is low, and the method is suitable for large-scale industrial production.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a low-cost extraction method of yeast oil. Background technique [0002] Traditional edible oils and fats mainly come from animals or oil plants. In the past ten years, due to the rapid population increase and rapid industrial development, the supply of oils and fats has become increasingly tense, ore resources have continued to decrease, and the energy crisis has continued to aggravate. The development of new oil resources and new energy It has attracted extensive attention from experts and scholars at home and abroad. Biodiesel can be produced from microbial oil through methyl esterification and transesterification. Compared with traditional petrochemical petroleum, biodiesel has the characteristics of good lubricating performance, safe storage and transportation, good antiknock performance, sufficient combustion, high energy density, renewable, easy biodegradation, lo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12R1/85
CPCC12P7/64
Inventor 车春玲
Owner JINAN DEV ZONE XINGHUO SCI & TECH RES INST
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