Serum-free animal cell culture medium dry powder, liquid culture medium and preparation method thereof

Abstract

The invention provides a serum-free animal cell culture medium dry powder and a preparation method thereof; the serum-free animal cell culture medium dry powder comprises basic culture medium dry powder and further comprises 100-200g / 100L of soy protein, 100-200g / 100L of pea protein, 100-200g / 100L of broad bean protein, 0-100g / 100L of potato protein, 0-200g / 100L of wheat gluten protein and 50-100g / 100L of rice protein by taking the volume of solvent of the culture medium as reference. The invention also provides a liquid serum-free animal cell culture medium containing the serum-free animal cell culture medium dry powder and a preparation method thereof. The cells cultured by the culture medium containing the vegetable protein are high in culture density, beneficial to separating down-stream products of the cells, low in cost, small in batch difference, good in safety and suitable for producing virus host, expression vector and the like of biological products such as vaccine and the like.

Description

technical field [0001] The invention relates to medium dry powder, liquid medium and their preparation methods, in particular to serum-free animal cell culture medium dry powder, liquid serum-free animal cell culture medium and their preparation methods. Background technique [0002] The prior art improves the culture stability and culture density of cultured host cells or recombinant cells, increases the yield of cultured host cells or recombinant cells, and increases the parasitic The yield of viruses, etc. in host cells or the yield of recombinant target protein products produced after using recombinant cells as expression vectors. And it is believed that hydrolysates of other plants, such as wheat hydrolysates, do not obtain comparable beneficial effects as soybean hydrolysates. [0003] For example, CN 1318577C (Patent No. 00816020.1) discloses a protein-free and serum-free medium for culturing mammalian cells, the medium contains soybean hydrolyzate, wherein at least ...

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Problems solved by technology

[0006] The purpose of the present invention is to overcome the shortcomings of the existing animal cell culture serum-free animal cell culture medium, such as low cell culture density, high cost of downstream products of separated cells, large batch differences of cultured cells, and poor safety, and provide a new Serum-free animal cell culture medium dry powder, made from serum-free animal cell culture medium dry powder, the cell culture density is high, which is conducive to the separation of downstream products of cells, low cost, small batch difference of cultured cells, and safety it is good

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