Method for fixing bone morphogenetic protein-2 (BMP-2) with decalcified bone matrix

A technology of demineralized bone matrix and bone morphoprotein, which can be used in medical science, prosthesis, etc. It can solve the problems of uneven fixation, unstable fixation, and prolong the activity of morphogenetic protein-2, so as to prolong the activity and improve the utilization rate. , delayed release effect
CN102133431AInactive Publication Date: 2011-07-27UNIV OF SHANGHAI FOR SCI & TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
UNIV OF SHANGHAI FOR SCI & TECH
Publication Date
2011-07-27
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for fixing a bone morphogenetic protein-2 (BMP-2) with a decalcified bone matrix. By using the decalcified bone matrix prepared by the metaphysis of pork hind leg bones as an object, the method comprises the following steps of: loading a BMP-2 solution at the concentration of 1 mu g per mu L to the decalcified bone matrix; placing into a microwave synthesizer, and processing for 30 minutes at the microwave power of 500 watts and reaction temperature 37 DEG C to fix the BMP-2 on the decalcified bone matrix; and putting the decalcified bone matrix in a 3 percentchitosan solution for 5 minutes, then taking the decalcified bone matrix out, and drying at the temperature of 37 DEG C. By adoption of the method provided by the invention, the fixation and combination of the BMP-2 and the decalcified bone matrix are stable, a fixing effect is good, the release speed of the BMP-2 is reduced, and the utilization ratio of the BMP-2 is improved; and the method has a higher practical application value in the field of medicine.
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Description

technical field

[0001] The invention relates to a method for fixing bone morphogenetic protein-2 and decalcified bone matrix, and belongs to the technical field of processing and manufacturing scaffold materials for repairing human bone tissue. Background technique

[0002] Demineralized bone matrix is ​​a commonly used bio-derived material for bone tissue engineering. It is derived from bone tissue and is a self-degraded antigen-inactivated xenogeneic or allogeneic bone. In 1965, Urist (American scientist Marshall R. Urist, the founder of orthopedic basic research) confirmed that demineralized bone can induce osteogenesis. Subsequently, the osteoinductive activity of decalcified bone has been confirmed by a large number of experimental studies and clinical practice. Decalcification can expose osteoinductive protein and activate the activity of osteoinductive protein, so that allogeneic bone can produce active osteoinductive ability, which has It is beneficial to the resorp...

Claims

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