Method for fixing bone morphogenetic protein-2 (BMP-2) with decalcified bone matrix

A technology of demineralized bone matrix and bone morphoprotein, which can be used in medical science, prosthesis, etc. It can solve the problems of uneven fixation, unstable fixation, and prolong the activity of morphogenetic protein-2, so as to prolong the activity and improve the utilization rate. , delayed release effect

Inactive Publication Date: 2011-07-27
UNIV OF SHANGHAI FOR SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention discloses a method for fixing bone morphogenetic protein-2 and decalcified bone matrix. The release amount of morphogenetic protein-2 and prolonging the activity of morphogenetic protein-2

Method used

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  • Method for fixing bone morphogenetic protein-2 (BMP-2) with decalcified bone matrix
  • Method for fixing bone morphogenetic protein-2 (BMP-2) with decalcified bone matrix
  • Method for fixing bone morphogenetic protein-2 (BMP-2) with decalcified bone matrix

Examples

Experimental program
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Effect test

Embodiment 1

[0022] Embodiment 1: Dip fixation method

[0023] 1. Prepare the demineralized bone matrix from the cancellous bone at the metaphysis of the porcine hind leg bone. The specific preparation process includes the following steps:

[0024] (1) Remove cartilage, cortical bone and attachment tissue on the surface of cancellous bone;

[0025] (2) Slicing and washing the cancellous bone obtained in (1);

[0026] (3) cutting the cancellous bone obtained in (2) into bone pieces of 1cm×1cm×3mm;

[0027] (4) the cancellous bone obtained in (3) was treated with Triton X-100 for 48 hours to remove the cells on the cancellous bone;

[0028] (5) treating the cancellous bone obtained in (4) with anhydrous ether for 48 hours to remove the fat and oil on the cancellous bone;

[0029] (6) Treat the cancellous bone obtained in (5) in 0.6 mol / L hydrochloric acid for 12 hours.

[0030] (7) Rinse the decalcified cancellous bone obtained in (6) with distilled water for 30 minutes to obtain a decal...

Embodiment 2

[0063] Embodiment 2: Negative pressure adsorption method

[0064] 1. Prepare the demineralized bone matrix from the cancellous bone at the metaphysis of the porcine hind leg bone. The specific preparation process includes the following steps:

[0065] (1) Remove cartilage, cortical bone and attachment tissue on the surface of cancellous bone;

[0066] (2) Slicing and washing the cancellous bone obtained in (1);

[0067] (3) cutting the cancellous bone obtained in (2) into bone pieces of 1cm×1cm×3mm;

[0068] (4) the cancellous bone obtained in (3) was treated with Triton X-100 for 48 hours to remove the cells on the cancellous bone;

[0069] (5) treating the cancellous bone obtained in (4) with anhydrous ether for 48 hours to remove the fat and oil on the cancellous bone;

[0070] (6) Treat the cancellous bone obtained in (5) in 0.6 mol / L hydrochloric acid for 12 hours.

[0071] (7) Rinse the decalcified cancellous bone obtained in (6) with distilled water for 30 minutes t...

Embodiment 3

[0105] Embodiment 3: microwave immobilization method

[0106] 1. Prepare the demineralized bone matrix from the cancellous bone at the metaphysis of the porcine hind leg bone. The specific preparation process includes the following steps:

[0107] (1) Remove cartilage, cortical bone and attachment tissue on the surface of cancellous bone;

[0108] (2) Slicing and washing the cancellous bone obtained in (1);

[0109] (3) cutting the cancellous bone obtained in (2) into bone pieces of 1cm×1cm×3mm;

[0110] (4) the cancellous bone obtained in (3) was treated with Triton X-100 for 48 hours to remove the cells on the cancellous bone;

[0111] (5) treating the cancellous bone obtained in (4) with anhydrous ether for 48 hours to remove the fat and oil on the cancellous bone;

[0112] (6) Treat the cancellous bone obtained in (5) in 0.6 mol / L hydrochloric acid for 12 hours.

[0113] (7) Rinse the decalcified cancellous bone obtained in (6) with distilled water for 30 minutes to ob...

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Abstract

The invention discloses a method for fixing a bone morphogenetic protein-2 (BMP-2) with a decalcified bone matrix. By using the decalcified bone matrix prepared by the metaphysis of pork hind leg bones as an object, the method comprises the following steps of: loading a BMP-2 solution at the concentration of 1 mu g per mu L to the decalcified bone matrix; placing into a microwave synthesizer, and processing for 30 minutes at the microwave power of 500 watts and reaction temperature 37 DEG C to fix the BMP-2 on the decalcified bone matrix; and putting the decalcified bone matrix in a 3 percentchitosan solution for 5 minutes, then taking the decalcified bone matrix out, and drying at the temperature of 37 DEG C. By adoption of the method provided by the invention, the fixation and combination of the BMP-2 and the decalcified bone matrix are stable, a fixing effect is good, the release speed of the BMP-2 is reduced, and the utilization ratio of the BMP-2 is improved; and the method has a higher practical application value in the field of medicine.

Description

technical field [0001] The invention relates to a method for fixing bone morphogenetic protein-2 and decalcified bone matrix, and belongs to the technical field of processing and manufacturing scaffold materials for repairing human bone tissue. Background technique [0002] Demineralized bone matrix is ​​a commonly used bio-derived material for bone tissue engineering. It is derived from bone tissue and is a self-degraded antigen-inactivated xenogeneic or allogeneic bone. In 1965, Urist (American scientist Marshall R. Urist, the founder of orthopedic basic research) confirmed that demineralized bone can induce osteogenesis. Subsequently, the osteoinductive activity of decalcified bone has been confirmed by a large number of experimental studies and clinical practice. Decalcification can expose osteoinductive protein and activate the activity of osteoinductive protein, so that allogeneic bone can produce active osteoinductive ability, which has It is beneficial to the resorp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/54A61L27/36
Inventor 王欣任智超周春梅刘宝林
Owner UNIV OF SHANGHAI FOR SCI & TECH
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