Artificial bone material having controlled calcium ion elution

A technology of artificial bone and calcium ions, applied in bone implants, medical science, prostheses, etc., can solve the problems of insufficient therapeutic effect and achieve the effect of inhibiting cytotoxicity and inflammatory response

Inactive Publication Date: 2011-07-27
NEXT21 KK
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Calcium-containing substances are widely used as biomaterials, but when they are used as biomaterials for treatment, there is a problem that sufficient therapeutic effects cannot be obtained

Method used

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  • Artificial bone material having controlled calcium ion elution
  • Artificial bone material having controlled calcium ion elution
  • Artificial bone material having controlled calcium ion elution

Examples

Experimental program
Comparison scheme
Effect test

manufacture example 1

[0092] Manufacture of artificial bone materials for transplantation

[0093] The inventors used α-TCP (manufactured by Taihei Science & Industry Co., Ltd.) as the main raw material to produce an artificial bone material for transplantation using Z printer-406 (Zprinter-406, manufactured by Zcorporation). This artificial bone material for implantation is immersed in a hardening solution. A 0.2 mol / L succinic acid aqueous solution (pH 6) was used as the hardening solution. After immersion in the hardening solution, the tetrapod type calcium ion precipitation control carrier was washed twice with Otsuka distilled water (trade name), and dried under reduced pressure in a vacuum low-temperature dryer (manufactured by Yamato Science Corporation) for 12 hours.

Embodiment 1

[0095] Study on Calcium Ion Increase and Cytotoxicity

[0096] Inhibition of Calcium Ion Precipitation in Artificial Bone Materials Treated with Trehalose

[0097] Artificial bone materials treated with trehalose and artificial bone materials not treated in this way were cultured with mouse osteoblast-like cells MC3T3-E1, and the cytotoxicity caused by the precipitation of calcium ions was evaluated by measuring cell proliferation . The medium used was Dulbecco's Modified Eagle Medium (D-MEM) containing 10% standard fetal bovine serum (FBS) and 1% penicillin-streptomycin. The artificial bone material for implantation (untreated tetrahedron) of Production Example 1 and the artificial bone material (treated tetrahedron) treated with a 4.5% trehalose solution were prepared. The untreated tetrahedron and the treated tetrahedron were added to each well of a 96-well multiwell plate. In addition, as a comparative experiment, mouse osteoblast-like cells MC3T3-E1 (0 tetrahedra) ...

Embodiment 2

[0100] Study on Chelating Effect of Gluconic Acid

[0101] In order to confirm the effect of gluconic acid on the chelation of calcium ions, a calcium ion electrode was used to measure the concentration of calcium ions in the presence of gluconic acid. The result is as figure 2 shown. figure 2 The vertical axis in represents the calcium ion (Ca 2+ ) concentration (mg / L). with 2.5mM CaCl 2 -0.25%~2.5% gluconic acid (Glu), (pH7.0) solution is adjusted, and it can be seen through concentration measurement that the higher the concentration of gluconic acid, the lower the concentration of calcium ions. This is the result of calcium ions being chelated by gluconic acid.

[0102] Therefore, by cleaning the artificial bone material for transplantation with a gluconic acid solution, the calcium ions attached to the surface of the artificial bone for transplantation etc. which are freed from the artificial bone material for transplantation can be effectively removed by chelatin...

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Abstract

Disclosed is an artificial bone material having controlled calcium ion elution, which does not induce cytotoxicity or any inflammatory response. It is found that the elution of a calcium ion from an artificial bone material for transplantation which contains a calcium-containing substance can be prevented effectively by subjecting the carrier to a surface treatment or adding a surface-treating agent to the carrier. It is also found that the induction of cytotoxicity can be prevented and the induction of an inflammatory response can also be prevented by using the above-mentioned carrier having controlled calcium ion elution.

Description

technical field [0001] The present invention relates to a medical artificial bone material (artificial bone material capable of controlling calcium ion precipitation) implanted in a living body. Background technique [0002] In the prior art, artificial bone materials are implanted in the human body for the purpose of treatment, and calcium-containing substances are widely used as such artificial bone materials. For example, Japanese Patent Laid-Open Publication No. 2006-346159 discloses a living tissue filling material, and calcium phosphate is used to manufacture the porous living tissue filling material. [0003] In addition, Japanese Patent Laid-Open Publication No. 2002-248119 discloses an artificial vertebral body containing hydroxyapatite and collagen. An artificial organ implant containing calcium phosphate is disclosed in WO2005 / 032456. [0004] Calcium-containing substances are widely used as biomaterials, but there is a problem that sufficient therapeutic effect...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/00A61L27/36A61L27/56
CPCA61L2430/02A61L27/12A61L27/50A61P29/00A61F2/28A61L27/02A61L27/54
Inventor 郑雄一佐佐木伸雄铃木茂树
Owner NEXT21 KK
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