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Monoclonal antibody of chloramphenicol and application thereof

A monoclonal antibody, chloramphenicol technology, applied in biochemical equipment and methods, instruments, microorganisms, etc., can solve problems such as normal flora imbalance and susceptibility to infection, and achieve strong controllability and repeatability.

Active Publication Date: 2013-04-24
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, long-term micro-intake of chloramphenicol not only makes Escherichia coli, Salmonella, etc. produce drug resistance, but also causes the imbalance of normal flora in the body, making people susceptible to various diseases

Method used

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  • Monoclonal antibody of chloramphenicol and application thereof
  • Monoclonal antibody of chloramphenicol and application thereof
  • Monoclonal antibody of chloramphenicol and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 1 material

[0019] 1.1 Animals and cell lines

[0020] BALB / c mice, Zhejiang Animal Experiment Center; F1 mice, Animal Experiment Center, Zhejiang University of Traditional Chinese Medicine; SP2 / 0 myeloma cells, Nanjing Keygen Biotechnology Development Co., Ltd.

[0021] 1.2 Main instruments

[0022] Laminar flow bench SCV-4A1, Streamline Laboratory Products; trinocular inverted phase contrast microscope ELWD0.3T1-SNCP, Nikon; carbon dioxide incubator 3111, Thermo Electron Corporation; HiTrap r-Protein A FF column (5mL), AKTA purifier, GE Healthcare; sterile culture plates, flasks, dishes, centrifuge tubes, Corning.

[0023] 1.3 Main reagents

[0024] Chloramphenicol, Shanghai Jingchun Reagent Co., Ltd.; HAT medium, HT medium, PEG(2000D), Sigma company; Fetal bovine serum, Minhai Bioengineering Co., Ltd.; RPML 1640 medium, Jinuo Biomedical Technology Co., Ltd. ; Goat anti-mouse IgG-HRP, Boster Biological Engineering Co., Ltd.

[0025] The immunization antigen CAP-...

Embodiment 2

[0054] Application of Chloramphenicol Monoclonal Antibody

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Abstract

The invention discloses a monoclonal antibody to chloramphenicol. The monoclonal antibody has specificity to chloramphenicol, and is secreted by a cell strain with the collection number of CCTCC 2010110. The monoclonal antibody to chloramphenicol can be applied to detecting residue of the chloramphenicol. The invention also provides a kit for detecting the residue of the chloramphenicol. The kit contains the monoclonal antibody to the chloramphenicol. The quality of the monoclonal antibody to the chloramphenicol has high controllability and repeatability. Through preliminary analysis and identification of the characteristics of the obtained antibody, an experimental foundation is provided for further establishment and application of a specific and sensitive community acquited pneumonia (CAP) immunoassay method.

Description

1. Technical field [0001] The invention provides an anti-chloramphenicol monoclonal antibody, in particular an anti-chloramphenicol monoclonal antibody prepared by using chloramphenicol-bovine serum albumin as an immunogen. 2. Background technology [0002] Chloramphenicol is a broad-spectrum antibiotic with good inhibitory effect on both Gram-negative and Gram-positive bacteria. Due to its excellent antibacterial properties, stable medicinal properties and low price, it is used in human and animal clinics as a therapeutic drug for bacterial diseases, and is widely used in animal husbandry and artificial aquaculture production as a feed additive. However, chloramphenicol is toxic and can easily cause blood poisoning in humans. Long-term use can lead to aplastic anemia and agranulocytosis. Long-term consumption of chloramphenicol-contaminated milk in infants may cause "grey baby syndrome". In addition, long-term micro-intake of chloramphenicol will not only make Escherichia...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K16/44C12N5/20G01N33/577C12R1/91
Inventor 应国清汪竹环易喻梅建凤陈建澍王鸿
Owner ZHEJIANG UNIV OF TECH