Method for raising deguelin content with rare earth element

A technology of rare earth elements and deterin, applied in the biological field, can solve the problems of slow growth of callus of deer vine, easy browning of deterin content, increased cost, etc., to improve growth status, reduce browning degree, The effect of increasing content

Inactive Publication Date: 2011-08-31
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, problems such as slow growth of callus of deervine plant, easy browning (browning rate of 50% to 60%), and low deterin content often appear in the study of degeria plant tissue culture. In large-scale production, Th

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0023] Example 1,

[0024] Wash the dirt and dust on the surface of the roe leaves with tap water, rinse with distilled water for 2 to 3 times, absorb the surface water with filter paper, soak in 75% alcohol for 30 seconds, rinse with sterile water for 2 to 3 times, use 2% Soak and sterilize with sodium hypochlorite solution for 6 minutes, and then wash with sterile water for 5-8 times. Use sterile filter paper to absorb the moisture on the surface of the leaves. Use a sterile punch to cut the leaves into small pieces of about one square centimeter and inoculate them respectively. Add 6.5g / L agar, 30g / L sucrose, 0.5mg / L cytokinin and 2mg / L auxin to MS plant medium (MS medium is currently the most commonly used medium. It has higher The concentration of inorganic salts can ensure the mineral nutrients needed for tissue growth and accelerate the growth of callus), placed in a 25°C incubator, protected from light and cultured for 20 to 30 days, and then subcultured. After three sub...

Example Embodiment

[0027] Example 2,

[0028] Inoculate the callus obtained by inducing and subculture 3 times of the roten leaves obtained in Example 1 into MS plant cell culture medium containing 0.005mM La, and add 6.5g / L agar per liter of medium, 30g / L sucrose, 6mg / L cytokinin and 6mg / L cytokinin, cultured in a 25°C incubator for 15 days, compared with the control group without rare earth elements, the callus obtained increased by 30%, while the brown The conversion rate has been reduced by 35%. Transfer the cultured callus to MS plant cell culture medium containing 0.01mM Ce, add 6.5g / L agar, 30g / L sucrose, 0.5mg / L cytokinin and 4mg / L cells per liter of culture medium Auxin, cultivated in a 25°C incubator in the dark for 35 days, compared with the control group without rare earth elements, the browning of callus was reduced by 27% and the content of rotenin was increased by 166%.

Example Embodiment

[0029] Example 3.

[0030] The callus obtained from the roe vine leaves obtained in Example 1 after being induced and subcultured 3 times was inoculated into 0.02 mM mixed rare earth elements (the composition ratio and the molar ratio were La 2 O 3 : CeO 2 : Pr 6 O 11 : Sm 2 O 3 =90:120:10:35) MS plant cell culture medium per liter of medium with 6.5g / L agar, 30g / L sucrose, 3mg / L cytokinin and 3mg / L cytokinin, at 30°C In the cultivator for 30 days, compared with the control group without rare earth elements, the callus browning decreased by 38% and the callus obtained increased by 45%. Transfer this callus to MS plant cell culture medium containing 0.05mM Ce, add 6.5g / L agar, 30g / L sucrose, 1mg / L cytokinin and 4mg / L cytokinin per liter of culture medium, When cultured in a 20°C incubator in the dark for 25 days, compared with the control group without rare earth elements, the browning of callus was reduced by 50% and the content of rotenin was increased by 150%.

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Abstract

The invention provides a method for raising deguelin content with rare earth element, comprising the steps of inoculating derris callus to routine plant cell medium containing 0.005 to 0.2mmol/L of rare earth element or rare earth element oxide, adding 6.5g/L of agar, 30 g/L of cane sugar, 0.5 to 6 mg/L of cytokinin, and 1 to 6mg/L of cell auxin for light-shading culture at a temperature of 20 DEG C to 30 DEG C for 15 to 30 days, inoculating derris callus once more to routine plant cell medium containing 0.005 to 0.2mmol/L of rare earth element or rare earth element oxide after the culture is finished, and adding 6.5g/L of agar, 30 g/L of cane sugar, 0.5 to 6 mg/L of cytokinin, and 1 to 6mg/L of cell auxin for light-shading culture at a temperature of 20 DEG C to 30 DEG C for 15 to 50 days. The invention improves the growing situation of derris callus cells, promotes the growth of derris cells, and raises the accumulation amount of deguelin by inoculating derris callus to culture medium containing rare earth elements.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for increasing the content of deguelin by using rare earth elements. Background technique [0002] fish vine ( Derris trifoliata Lour) is the genus of Fabaceae Deervine ( Derris Lour ) plants, there are more than 80 species of this genus in the world, mainly distributed in tropical and subtropical regions, and about 20 species are found in my country, widely distributed in Fujian, Guangdong, Yunnan, Taiwan and other provinces in China. Deerides ( Derris Lour ) leguminous plants such as Demeritus ( Derris trifoliata Lour.), Maoyu vine ( Derris elliptica (Roxb.) Benth.), and Ashleaf ( Tephrosia Pers ) leguminous plant edamame ( Tephrosia vogelii Hook f. ) and so on contain a large amount of rotenone compounds, which are the main source of rotenone compounds. [0003] Deterin is one of the rotenone compounds. Recent studies have shown that deterin ca...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 甘纯玑陈潇彬李坚黄钰洁谢苗
Owner FUJIAN AGRI & FORESTRY UNIV
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