Method for raising deguelin content with rare earth element

Abstract

The invention provides a method for raising deguelin content with rare earth element, comprising the steps of inoculating derris callus to routine plant cell medium containing 0.005 to 0.2mmol / L of rare earth element or rare earth element oxide, adding 6.5g / L of agar, 30 g / L of cane sugar, 0.5 to 6 mg / L of cytokinin, and 1 to 6mg / L of cell auxin for light-shading culture at a temperature of 20 DEG C to 30 DEG C for 15 to 30 days, inoculating derris callus once more to routine plant cell medium containing 0.005 to 0.2mmol / L of rare earth element or rare earth element oxide after the culture is finished, and adding 6.5g / L of agar, 30 g / L of cane sugar, 0.5 to 6 mg / L of cytokinin, and 1 to 6mg / L of cell auxin for light-shading culture at a temperature of 20 DEG C to 30 DEG C for 15 to 50 days. The invention improves the growing situation of derris callus cells, promotes the growth of derris cells, and raises the accumulation amount of deguelin by inoculating derris callus to culture medium containing rare earth elements.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for increasing the content of deguelin by using rare earth elements. Background technique [0002] fish vine ( Derris trifoliata Lour) is the genus of Fabaceae Deervine ( Derris Lour ) plants, there are more than 80 species of this genus in the world, mainly distributed in tropical and subtropical regions, and about 20 species are found in my country, widely distributed in Fujian, Guangdong, Yunnan, Taiwan and other provinces in China. Deerides ( Derris Lour ) leguminous plants such as Demeritus ( Derris trifoliata Lour.), Maoyu vine ( Derris elliptica (Roxb.) Benth.), and Ashleaf ( Tephrosia Pers ) leguminous plant edamame ( Tephrosia vogelii Hook f. ) and so on contain a large amount of rotenone compounds, which are the main source of rotenone compounds. [0003] Deterin is one of the rotenone compounds. Recent studies have shown that deterin ca...

AI Technical Summary

Problems solved by technology

However, problems such as slow growth of callus of deervine plant, easy browning (browning rate of 50% to 60%), and low deterin content often appear in the study of degeria plant tissue culture. In large-scale production, The inability to obtain an efficient and stable culture system will undoubtedly increase the cost of production, so it is very important and necessary to establish an efficient and stable plant cell culture system in production