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Common anemarrhena polysaccharide extractive and preparation method and medicinal purposes thereof

A polysaccharide extraction and extraction technology, which is applied in the direction of pharmaceutical formulations, drug combinations, plant raw materials, etc., can solve the problems of less research on Anemarrhena polysaccharides and no immunosuppressive activity

Active Publication Date: 2011-08-31
匡海学
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Modern research has found that the chemical components in Anemarrhena are mainly steroidal saponins and bivapizones, and there are also lignans, flavonoids, organic acids, etc., which is one of the hot drugs in modern medical research. In recent years The research progresses rapidly, but most of them focus on timosaponins and bivapirox components. It is believed that timosaponin is the important pharmacodynamic material basis of timosapones. There are few studies on timosapones, and it has not been found that timosapones have immunosuppressive effects. active report

Method used

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  • Common anemarrhena polysaccharide extractive and preparation method and medicinal purposes thereof
  • Common anemarrhena polysaccharide extractive and preparation method and medicinal purposes thereof
  • Common anemarrhena polysaccharide extractive and preparation method and medicinal purposes thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0016] Take about 2.8 kg of Anemarrhena medicinal material, reflux extraction with 90% ethanol for 3 times, 2 hours each time, filter, dry the filter residue, add 10 times the amount of distilled water to the residue, reflux extraction 3 times, 2 hours each time, filter, combine the filtrate, reduce Recover the solvent to 1 times the volume of the medicinal material, adjust the alcohol content to 80% with 95% ethanol, let stand overnight, centrifuge at 2000rpm / min, and wash the precipitate with 4 times the amount of absolute ethanol and acetone in turn to obtain the total polysaccharide. Take the total polysaccharide and add water to dissolve, put the supernatant into a dialysis bag for 48 hours of running water dialysis, 24 hours of distilled water dialysis, reduce pressure and concentrate the dialysate to 1 volume of medicinal materials, add 95% ethanol to adjust the alcohol content to 85%, let stand overnight, 2000rpm / Min centrifugation, the precipitate was washed sequentia...

Embodiment 2

[0018] Take about 3 kg of Anemarrhena medicinal material, reflux extraction with 80% ethanol for 2 times, each time for 3 hours, filter, dry the filter residue, add 8 times the amount of distilled water to the residue, reflux extraction twice, each time for 3 hours, filter, combine the filtrate, and reduce pressure Recover the solvent to 1 times the volume of the medicinal material, adjust the alcohol content to 80% with 95% ethanol, let it stand overnight, centrifuge at 2000rpm / min, and wash the precipitate with 3 times the amount of absolute ethanol and acetone in turn to obtain the total polysaccharide. Dissolve the total polysaccharide in water, dialyze the supernatant under running water for 48 hours, dialyze with distilled water for 24 hours, concentrate the dialysate under reduced pressure to 1 volume of medicinal materials, add 95% ethanol to adjust the alcohol content to 80%, let it stand overnight, centrifuge at 2000rpm / min, and use it for precipitation Wash with 3 ti...

Embodiment 3

[0019] Embodiment 3 is made transfusion

[0020] Anemarrhena total polysaccharides were further purified and separated through Sepharose column Sephacryl S300 and Sephadex G50 chromatography: to obtain anemarrhena polysaccharide extracts with a purity of more than 98%, add an appropriate amount of solubilizer, grind, then add a small amount of water for injection to dilute, mix Mix well, then add an appropriate amount of sodium chloride, dissolve and then add water for injection to the specified amount, filter, pot, and sterilize to obtain.

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PUM

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Abstract

The invention relates to a common anemarrhena polysaccharide extractive with immunosuppression activity and a preparation method and medicine purposes thereof. The preparation method comprising the steps of degreasing raw materials, extracting the degreased raw materials with water, precipitating the extracted material with alcohol, carrying out dialysis and ion-exchange column chromatography on the crude polysaccharide to eliminate proteins, pigments and molecular chemical components to produce an anemarrhena extractive. A huge amount of pharmacological experiments prove that common anemarrhena polysaccharide has an immunodepression activity and has the medical purposes of treating proinflammatory traumatic diseases, such as nephritis, pneumonia, upper respiratory tract infection, hepatitis, tonsillitis and diabetes.

Description

technical field [0001] The present invention relates to an anemarrhena polysaccharide extract and its preparation method, and the anemarrhena polysaccharide extract is used as immunosuppressive activity for the treatment of immune inflammatory injury diseases: nephritis, pneumonia, upper respiratory tract infection, hepatitis, tonsil Medicine for inflammation and diabetes. Background technique [0002] Anemarrhena is the rhizome of Anemarrhena asphodeloides Bge., which is bitter, sweet, and cold in nature, and belongs to the lung, stomach, and kidney channels. It has the functions of clearing heat and purging fire, nourishing yin and moistening dryness. It is used for febrile diseases caused by exogenous pathogenesis, high fever and polydipsia, dry cough due to lung heat, hot flashes due to bone steaming, internal heat quenching thirst, intestinal dryness and constipation. Modern research has found that the chemical components in Anemarrhena are mainly steroidal saponins an...

Claims

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Application Information

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IPC IPC(8): A61K36/8964C08B37/00A61P1/16A61P3/10A61P11/00A61P11/04A61P13/12A61P37/06
Inventor 匡海学王秋红王知斌夏永刚穆光锐薛娟
Owner 匡海学
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