Preparation method of compound santhoceraside

A technology of hucoside and compound, which is applied in the field of medicine, can solve the problems such as the preparation method of juvenile husk that has not been seen before, and achieves the effect of protecting brain cells.

Inactive Publication Date: 2011-09-14
SHENYANG PHARMA UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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  • Preparation method of compound santhoceraside

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Embodiment 1

[0014] 1) Take crushed 20-mesh 1000g sorbifolia fruit shell and stalk raw materials, extract with 5 times the 65% concentration of ethanol, and keep the temperature in a slightly boiling state for 2.5 hours, stirring for 5 minutes at intervals of 20 minutes, total Extract 3 times.

[0015] 2) Combine the three extracts, recover ethanol, pass the filtered water through AB-8 macroporous resin to remove sugar, elute with 45% ethanol, and recover the ethanol in the eluent.

[0016] 3) Adsorb the pigment with magnesium oxide for decolorization.

[0017] 4) The decolorized aqueous solution is extracted with ethyl acetate and n-butanol, and the solvent is recovered to obtain a two-part extract of ethyl acetate and n-butanol.

[0018] 5) Put the n-butanol part on a silica gel column, and carry out gradient elution with chloroform:methanol=100:0-65, recover the eluate, and recrystallize with acetone to obtain white crystals, namely Xanthophyllin, mp: 267-268°C, a spot appears on the ...

Embodiment 2

[0020] 1) Take crushed 40-mesh 1000g sorbifolia husk and stalk raw materials, extract with 7 times the 75% concentration of ethanol, the temperature is 100°C, keep boiling, the extraction time is 3 hours, continuous stirring, co-leaching Mention 3 times.

[0021] 2) Combine 3 times of extracts, recover ethanol, pass filtered water through AB-8 macroporous resin column, remove sugar and enrich active ingredients, elute with 60% ethanol, recover eluent ethanol.

[0022] 3) Remove the pigment with activated carbon.

[0023] 4) The decolorized solution is extracted with ethyl acetate and n-butanol, and the solvent is recovered to obtain a two-part extract of ethyl acetate and n-butanol.

[0024] 5) Put the n-butanol part on a silica gel column, and carry out gradient elution with chloroform:methanol=100:10-65, recover the eluate, crystallize with methanol, and obtain white crystals, which are sorbetin, mp: 267 -268°C, a spot is displayed on the thin-layer chromatography plate, ...

Embodiment 3

[0026] 1) Take 60 meshes and 1000g of Xanthos sorbifolia husk and stem raw materials, extract with 10 times the 80% concentration of ethanol, the temperature is 95°C, the extraction time is 4 hours, continuous stirring, and a total of 3 extractions.

[0027] 2) Combine the extracts for 3 times, recover ethanol, pass the filtered water through AB-8 macroporous resin column, remove sugar and enrich active ingredients, elute with 75% ethanol, and recover the eluate of ethanol.

[0028] 3) Decolorize with activated carbon and activated clay.

[0029] 4) The solution after decolorization is extracted with ethyl acetate and n-butanol in sequence, and the solvent is recovered to obtain two parts of extract of ethyl acetate and n-butanol.

[0030] 5) The n-butanol part was prepared by HPLC to obtain white crystals, mp: 267-268°C, a spot appeared on the thin-layer chromatography plate, and the color was developed with 10% sulfuric acid ethanol solution, which was Purple spot, same as...

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Abstract

The invention relates to the field of medicine and relates to a preparation technology of santhoceraside, in particular to a method for extracting santhoceraside. The method comprises the following steps of: smashing xanthoceras sorbifolia bunge shells and stalks; extracting with an organic solvent; filtering; recycling the organic solvent; making the solution flow through macroporous resin; leaching with ethyl acetate and normal butanol in sequence; and preparing santhoceraside, i.e., 3-O-(a-L-arabinofuranosyl(1->3)-b-D-galactopyranosyl(1->2))-b-D-glucuronopyranosyl-21,22-diangeloyl-R1-barrigenol with a silica gel column or a high performance liquid chromatography. The structural formula of the santhoceraside is shown in the specification.

Description

technical field [0001] The invention belongs to the technical field of medicines, and relates to the utilization technology of sorbifolia husk and stalk, in particular to a method for extracting the active ingredient radishes sorbiflora glycosides in the sorbifolia husk and stalk. Background technique [0002] Xanthoceras sorbifolia Bunge is a unique oil and folk medicinal plant in my country. It only belongs to one species and is mainly distributed in Liaoning, Inner Mongolia and the Hexi Corridor. Xanthoceras sorbifolia Bunge is a deciduous shrub or tree with drought tolerance, It has excellent characteristics such as cold resistance and barren resistance, and is an ideal tree species for protecting the environment and greening the environment. The seedlings will bloom and bear fruit after 2-3 years of growth. [0003] The seed kernel of X. sorbifolium contains 60% oil, which is edible, medicinal, and can also be used as industrial oil. The country has listed X. sorbifolium...

Claims

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Application Information

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IPC IPC(8): C07J63/00
Inventor 邹莉波迟天燕纪雪飞
Owner SHENYANG PHARMA UNIVERSITY
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