Detection method and detection kit for detecting special pathogenic bacteria by bioluminescence

A bioluminescent, pathogenic bacteria technology, applied in the field of microbial detection, to achieve the effect of strong specificity, high sensitivity, and easy operation
CN102183648AInactive Publication Date: 2011-09-14SHANGHAI INST OF MICROSYSTEM & INFORMATION TECH CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SHANGHAI INST OF MICROSYSTEM & INFORMATION TECH CHINESE ACAD OF SCI
Publication Date
2011-09-14
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a method and a detection kit for detecting special pathogenic bacteria by a bioluminescence method combined with immunomagnetic bead identification. According to the method of the invention, with monoclonal antibodies or polyclonal antibodies on an immunomagnetic bead, bacteria in a bacterial suspension or a sample are captured by antigen-antibody reactions; adenosine triphosphate (ATP) in the bacteria is released by adding a lysate; finally detecting the ATP content by fluorescein (D-Luciferin)-luciferase so as to determine whether specific microorganism is present in the sample, and determining the amount of the contained specific microorganism by the determination of an ATP standard curve. The kit provided by the invention comprises an immunomagnetic bead coating the antibodies, a microbial lysate, luciferase and a protective agent, a detection buffer, etc. With the invention, detection time can be shortened greatly (within 2 hours); no bacterium increasing or filtering is necessary; the operation is simple; the sensitivity is high; the specificity is high; and the invention is applicable to rapid field detection of food and environment samples, and basic popularization and application.
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Description

technical field

[0001] The invention relates to a detection method and a detection kit for bioluminescent detection of special pathogenic bacteria. More precisely, the present invention uses a bioluminescence method to quickly detect the total amount of microorganisms or the number of specific microorganisms and the detection kit, which belong to microorganisms detection field. Background technique

[0002] Rapid detection and identification of pathogenic bacteria is an important issue in today's society, whether it is in clinical diagnosis, foodborne pathogen monitoring, or even biological weapon prevention. There are many kinds of foodborne pathogenic microorganisms, and the lack of sensitive, convenient and specific rapid detection technology is one of the main reasons why food safety and people's health cannot be effectively guaranteed. Due to the lack of powerful monitoring technology, according to the WHO report, the reported incidence of foodborne diseases in countri...

Claims

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