Application of chrysopogon zizanioides in eutrophic water
A technology of eutrophication and vetiver grass, applied in chemical instruments and methods, biological water/sewage treatment, water/sludge/sewage treatment, etc., can solve the problems of secondary environmental pollution, poor effect, and difficult operation. , to achieve the effect of improving water quality
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Embodiment 1
[0017] Example 1: The algal inhibitory effect of Vetiver
[0018] 1.1 Test material
[0019] Vetiver (Vetiveria zizanioides (L.) Nash.) was collected from the Vetiver Planting Base in Shuifan Village, Changpu Town, Yuexi County, Anhui Province.
[0020] The test algal seed toxicity Microcystis aeruginosa (Microcystis aeruginosa) was provided by the School of Life Sciences, Anhui Normal University, and BG-11 medium was used for algal seed culture.
[0021] The composition of BG-11 medium is: NaNO 3 , 1.5g / L, K 2 HPO 4 ·3H 2 O, 0.04g / L, MgSO 4 ·7H 2 O, 0.075g / L, CaCl 2 ·2H 2 O, 0.036g / L, Citric Acid, 0.006g / L, Ferric Ammonium Citrate, 0.006g / L, EDTA, 0.001g / L, Na 2 CO 3 , 0.02g / L, distilled water, 919mL / L.
[0022] Cultivation of algal seeds: Under sterile conditions, add 100 mL of culture medium to each of the washed and sterilized triangular conical flasks, insert the algal seeds, shake well, and place in a light incubator for cultivation. Culture conditions: illum...
Embodiment 2
[0062] Example 2: Removal of algal toxins by vetiver.
[0063] 2.1 Test material
[0064] Vetiver (Vetiveria zizanioides (L.) Nash. was derived from Example 1 .
[0065] 2.2 Reagents
[0066] The cyanobacterial toxin MC-LR is provided by the Department of Biochemistry of An Normal University, and is a product of the Wuhan Institute of Hydrobiology, Chinese Academy of Sciences.
[0067] 2.3 Absorption experiment of MC-LR by roots and leaves of vetiver
[0068] Select the newly grown roots and the newly grown young leaves from the roots from the vetiver grass after culturing for 10 days, wash and rinse with distilled water 3 times, and then absorb the water with filter paper (the operation is carried out on the ultra-clean workbench) Carry out algal toxin absorption experiments.
[0069] Sterilized MC-LR was added to the BG11 medium, and the concentrations of MC-LR were 1, 5 and 10 μg / L, respectively. Immerse the roots or leaves of 2g fresh weight in 100mL of MC-LR solution...
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