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Method for extracting peanut polysaccharides from hot pressed peanut meal

A technology for hot-pressed peanut meal and polysaccharide, which is applied in the directions of antitoxins, metabolic diseases, antitumor drugs, etc., can solve problems such as unsatisfactory extraction rate, avoid damage to the activity and safety of polysaccharides, mild reaction conditions, and improve extraction. The effect of efficiency

Inactive Publication Date: 2011-09-28
山东创新源农业技术开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In order to solve the problem of unsatisfactory extraction rate in the previous scheme, the present invention uses non-toxic and efficient biological enzymes to extract polysaccharides from peanut meal. By adjusting the structure and dosage of biological enzymes, the functional characteristics of polysaccharides are kept to the greatest extent, avoiding the Contamination by organic solvents improves the extraction efficiency of polysaccharides

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The hot-pressed peanut meal is ultrafinely pulverized to 350 mesh, the raw material of the hot-pressed peanut meal is refluxed in 100ml of petroleum ether at 50°C for 8 hours, the petroleum ether is separated, and the hot-pressed peanut meal is dried to obtain dry powder of the hot-pressed peanut meal.

[0021] A citric acid-sodium citrate buffer solution system with a concentration of 0.1 mol / l is added to the dry powder of hot-pressed peanut meal, and the mass of the buffer solution added is 10 times the mass of the dry powder of hot-pressed peanut meal. Add cellulase, papain, acid protease to enzymatically hydrolyze peanut meal, the amount of added cellulase is 0.6% of the mass of hot-pressed peanut meal dry powder, the amount of papain is 0.05% of the mass of hot-pressed peanut meal dry powder, acid protease The dosage is 0.45% of the mass of hot-pressed peanut meal dry powder, and it is enzymatically hydrolyzed at 40°C and pH 5 for 2 hours.

[0022] After the enzym...

Embodiment 2

[0027] The hot-pressed peanut meal is ultrafinely pulverized to 400 mesh, the raw material of the hot-pressed peanut meal is refluxed in 100ml of petroleum ether at 50°C for 8 hours, the petroleum ether is separated, and the hot-pressed peanut meal is dried to obtain dry powder of the hot-pressed peanut meal.

[0028] Add a citric acid-sodium citrate buffer solution system with a concentration of 0.1mol / l to the hot-pressed peanut meal dry powder. The quality of the buffer solution added is 20 times the mass of the peanut meal dry powder. To decompose peanut meal, the added cellulase consumption is 0.6% of the dry powder quality of hot-pressed peanut meal, the consumption of papain is 0.15% of the dry powder quality of hot-pressed peanut meal, and the consumption of acidic protease is 0.6% of the dry powder quality of hot-pressed peanut meal. Enzymolysis was carried out at 50°C and pH7 for 250 minutes.

[0029] After the enzymatic hydrolysis, the reaction system was placed in ...

Embodiment 3

[0034] The hot-pressed peanut meal is ultrafinely pulverized to 500 mesh, the raw material of the hot-pressed peanut meal is refluxed in 100ml of petroleum ether at 50°C for 8 hours, the petroleum ether is separated, and the hot-pressed peanut meal is dried to obtain dry powder of the hot-pressed peanut meal.

[0035] A citric acid-sodium citrate buffer solution system with a concentration of 0.08 mol / l is added to the dry powder of peanut meal, and the mass of the buffer solution added is 20 times that of the dry powder of peanut meal. Add cellulase, papain, acid protease to enzymatically hydrolyze peanut meal, the amount of added cellulase is 0.7% of the mass of hot-pressed peanut meal dry powder, the amount of papain is 0.1% of the mass of hot-pressed peanut meal dry powder, acid The amount of protease is 0.6% of the mass of hot-pressed peanut meal dry powder. Enzymolysis was carried out at 50° C. and pH 5 for 3 hours.

[0036] After the enzymatic hydrolysis, the reaction ...

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PUM

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Abstract

The invention belongs to the field of food processing methods, in particular to a method for extracting peanut polysaccharides from hot pressed peanut meal. The method comprises the following steps of: pretreating the peanut meal; extracting the polysaccharides; separating and purifying; and drying, wherein the polysaccharides are extracted from the peanut meal by a method of performing enzymolysis on the hot pressed peanut meal by cellulose, papain and acid protease, the cellulose, the papain and the acid protease account for 0.6 to 0.8 percent, 0.05 to 0.15 percent and 0.45 to 0.6 percent of the mass of dry powder of the hot pressed peanut meal respectively, the temperature is 30-50DEG C, the pH is 4-6 and the time is 1.5-5 hours. The peanut polysaccharides are extracted from the hot pressed peanut meal by enzyme and enzymolysis processes, the reaction conditions are mild, the polysaccharides extracting efficiency can be improved to the greatest extent, the activity of the polysaccharides can be maintained to the greatest extent, the damage of traditional strong acid, strong base and other organic solvents to the activity and safety of the polysaccharides is avoided, and a high-efficiency, circulable, environment-friendly and low-carbon way is provided for comprehensive utilization of industrial byproducts.

Description

technical field [0001] The invention belongs to the field of food processing methods, and in particular relates to a method for extracting peanut polysaccharides from hot-pressed peanut meal. Background technique [0002] Peanut meal is one of the main by-products of grain and oil processing enterprises. At present, peanut meal is generally used as feed for fish and poultry without further development and utilization, resulting in a great waste of resources. The polysaccharide content in peanut meal is 32.50%. Studies have shown that polysaccharide has a series of functional activities such as anti-oxidation, anti-tumor, lowering blood fat, and improving immune function of the body. If the polysaccharide in peanut meal can be extracted, it will greatly improve peanut processing. Use of added value. [0003] At present, most of the methods used to extract peanut polysaccharides from peanut meal are: extracting peanut polysaccharides with acid or alkali, and using ultrasonic ...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P39/06A61P35/00A61P3/06A61P37/02
Inventor 段友臣邓鹏杜方岭王文亮
Owner 山东创新源农业技术开发有限公司