Method for producing biodiesel with no by-product of glycerin, under catalysis of immobilized enzyme
A technology for immobilizing enzymes and biodiesel, applied in renewable new energy and green fields, can solve the problems of waste of resources, pollute the environment, aggravate environmental pollution, etc., achieve the effects of easy separation, improve utilization rate, and solve technical and economic burdens
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Embodiment 1
[0028] Add 5g of palm oil into a stoppered Erlenmeyer flask containing 8.5g of dimethyl carbonate, heat it to 60°C in a constant-temperature water-bath shaker, then add 1g of immobilized lipase Novozyme435 into the Erlenmeyer flask, and react at constant temperature for 24 hours. The catalyst was separated and recovered by filtration, and the filtrate was removed by rotary evaporation to remove excess dimethyl carbonate, and no glycerol stratification appeared after standing. The product was quantitatively analyzed by gas chromatography, and no free glycerin was detected. The yield of biodiesel reached 92.5%. .
Embodiment 2
[0030] Add 5 g of palm oil into a stoppered Erlenmeyer flask filled with 8.5 g of dimethyl carbonate, heat it to 60° C. in a constant temperature water bath shaker, then add 1 g of immobilized lipase Novozyme 435 recovered from Example 1 into the Erlenmeyer flask In the process, after constant temperature reaction for 24 hours, the catalyst was separated by suction filtration, and the filtrate was removed by rotary evaporation to remove excess dimethyl carbonate, and there was no glycerin stratification on standing. The product was quantitatively analyzed by gas chromatography, and no free glycerin was detected. The yield yield reached 93.0%.
[0031] According to the methods of Examples 1 and 2, the immobilized enzyme catalyst was used more than 20 times, and the yield of biodiesel remained at about 90%.
Embodiment 3
[0033] Add 5 g of palm oil into a stoppered Erlenmeyer flask filled with 13.93 g of diethyl carbonate, heat it to 40°C in a constant temperature water bath shaker, add 1 g of immobilized lipase Lipozyme TL IM, and react at constant temperature for 18 hours. The catalyst was separated and recovered by filtration, and excess diethyl carbonate was removed by rotary evaporation in the filtrate, and no glycerol stratification appeared on standing. The product was quantitatively analyzed by gas chromatography, no glycerin was detected, and the yield of biodiesel was 93.2%.
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