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Method for quickly growing seedling of wild buckwheat rhizome by tissue culture

A tissue culture, golden buckwheat technology, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of golden buckwheat tissue culture seedling refining and transplanting technology research, the stem should not be induced to root, and the browning phenomenon deepens. , to achieve the effect of maintaining genetic stability, shortening the breeding cycle, and easy to obtain materials

Inactive Publication Date: 2012-12-19
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the study pointed out that the level of cytokinin 6-BA should not be too high, too high will stimulate the activity of polyphenol oxidase in explants, thereby deepening the browning phenomenon; it is also prone to vitrification, and its stems should not be induced Rooting, the reproduction coefficient is greatly reduced
More importantly, this study did not study the seedling hardening and transplanting technology of golden buckwheat tissue culture seedlings, and the transplanting survival rate of tissue culture seedlings is the key to whether this technology can really be applied to commercial production

Method used

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  • Method for quickly growing seedling of wild buckwheat rhizome by tissue culture
  • Method for quickly growing seedling of wild buckwheat rhizome by tissue culture
  • Method for quickly growing seedling of wild buckwheat rhizome by tissue culture

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Experimental program
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Embodiment 1

[0029]The young golden buckwheat stems introduced from Jiangsu and cultivated in the experimental base of the Institute of Medicinal Plants for many years were used as explants, cut into 2-3cm sections, treated with 70% alcohol for 30 seconds, rinsed with sterile water twice, and then Soak in 0.1% mercury liter solution for 12 minutes, shake constantly during the period, and finally rinse with sterile water 5 times, 1 minute each time. The explants treated above were cut into 30 single-bud stem segments, inoculated in MS medium containing 2.0mg / L 6-BA+0.5mg / LTDZ+0.2mg / L NAA+30% sucrose, at 25 ±2°C, 1500-3000Lux, culture under 12-16h / d light to induce and proliferate adventitious buds, and obtain clustered seedlings (such as figure 1 ), subcultured once every 30 days, and a large number of adventitious sprouts were obtained after subcultured 3 times. Select robust rootless seedlings of about 2-3 cm, and transfer them to a rooting medium to induce rooting. The formula of the ro...

Embodiment 2

[0031] The young golden buckwheat stems introduced from Guizhou and cultivated in the experimental base of the Institute of Medicinal Plants for many years were used as explants, cut into 2-3cm long sections, treated with 70% alcohol for 30 seconds, and washed twice with sterile water , and then soaked in 0.1% mercuric chloride solution for 10 minutes, shaking constantly during the period, and finally rinsed with sterile water 5 times, 1 minute each time. The explants treated above were cut into 30 single-bud stem segments, inoculated in MS medium containing 1.0mg / L6-BA+0.5mg / L NAA+30% sucrose, at 25±2°C, 1500- 3000Lux, culture under 12-16h / d light to induce adventitious buds, after 2 weeks axillary buds germinate to form sterile seedlings. The obtained sterile seedlings are further cut into single-bud stem segments, and transferred to the bud proliferation medium obtained by screening. The composition of the culture medium is: MS+2.0mg / L 6-BA+0.2mg / L TDZ+0.5mg / L NAA+30% sucr...

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Abstract

The invention provides a method for quickly growing a seedling of a medicinal plant of wild buckwheat rhizome by tissue culture. The method comprises the following steps of: selecting a budded stem section of the wild buckwheat rhizome as an explant, sterilizing the surface, then inoculating the explant to an MS (Murashige and Skoog) culture medium with 2.0 mg / L of 6-BA (6-benzylaminopurine), 0.5mg / L of TDZ (Thidiazuron), 0.2 mg / L of NAA (Naphthaleneacetic Acid) and 30% of cane sugar to induce quick propagation of adventitious buds, and subculturing three times to obtain a large amount of adventitious bud seedlings; transferring the adventitious bud seedlings about 2-3 cm into the culture medium with 1 / 2MS, 0.5 mg / L of LNA (Linolenic Acid) and 20% of cane sugar to induce rooting; and training the seedling, then transplanting to a field, wherein the transplanting survival rate of a test-tube plantlet is more than 90%. The method has the advantages of high speed, high efficiency, low cost, high transplanting survival rate, convenience for popularization, favorable genetic stability and is suitable for various wild buckwheat rhizome cultivation breeds or types. A large amount of high-quality wild buckwheat rhizome germchits and raw medical herbs can be propagated and produced in a short period of time according to market demands.

Description

technical field [0001] The invention relates to plant regeneration through tissue culture technology, in particular to a rapid seedling raising method of the medicinal plant golden buckwheat through tissue culture. Background technique [0002] At present, malignant tumors have become one of the major diseases that are increasingly common and seriously threaten human life and quality of life. Therefore, the mechanism and development of anticancer drugs and cancer prevention drugs have become a hot research topic for scientists in recent years. Golden buckwheat (Fagopyrum dibotrys (D.Don) Hara) is a perennial herb of the genus Buckwheat in the family Polygonaccae. It is a resource plant with rich nutrition and important medicinal value. The root of golden buckwheat is a traditional Chinese herbal medicine in my country. According to the "Compendium of Materia Medica", it can "lower qi and widen the intestines, grind stagnant stagnation, clear away heat and swelling, remove ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 李艾莲陈彩霞
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI