Method for separating naphthoquinone active ingredients from sinkiang arnebia root
A technology for the separation of active ingredients, applied in the field of extraction of naphthoquinones, the anti-tumor active ingredient of comfrey, can solve the problems of large solvent consumption, long separation time, and many impurities in the extraction, and achieve large sample loading and instrument The effect of requiring low configuration and low operating cost
Inactive Publication Date: 2013-01-02
WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
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Problems solved by technology
[0003] At present, there are many research reports on the extraction and purification methods of shikonadhiquinone components, but most of them are traditional extraction and separation processes, with long separation time and many extraction impurities; large solvent consumption; difficult to separate and obtain high-purity medicinal components
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[0020] A method for extracting the antitumor active ingredient-naphthoquinones of comfrey, the concrete steps are:
[0021] A. Dry and crush 1kg of dried comfrey stems, and then use ethanol for ultrasonic extraction. The amount of ethanol poured in is 5 to 10 times the weight of the medicinal material. Place in an ultrasonic instrument and sonicate for 30 or 34 or 39 or 44 or 47 or 50 minutes, then filter the sonicated substance, and recover the solvent in the filtered solution. Pour the recovered solvent into the conical flask again, continue ultrasonication, and repeat this step 3 or 4 or 5 times to obtain 55 g of dry extract extracted with ethanol, and the yield is 5.5% of the weight of the dry stem of Comfrey.
[0022] B, extract the ethanol extract with solvent sherwood oil and ethyl acetate respectively, the amount of extraction solvent sherwood oil and ethyl acetate is 4 or 5 or 6 or 7 times of extractum, extract to liquid colorless, respectively with sherwood oil and ...
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The invention discloses a method for isolating effective components of naphthoquinones from comfrey, the steps of which are: A, drying the dried stems of comfrey, ultrasonically extracting with ethanol, and supersonicating to obtain a dry extract; B, using a solvent Petroleum ether and ethyl acetate extract the ethanol dry extract to obtain petroleum ether and ethyl acetate extraction extract; C. Ultrasonic dissolve the extract with an equal volume of ethyl acetate, evaporate to dryness until there is no ethyl acetate smell, and connect it in series The column of the medium and low pressure chromatographic column is connected to the medium and low pressure preparative chromatographic column; D, the eluent is petroleum ether, and the column is eluted under the screen monitoring picture of the medium and low pressure chromatographic column, and the fraction is collected to obtain the compound; E, the medium and low pressure preparative chromatographic column In series, the compound is dissolved in methanol, and the compound is purified; F, the compound produces purple after alkaline reaction, and the colorless methylene blue color test is positive. The method is easy to implement and easy to operate. The method is to use middle and low pressure preparation chromatography; an optimized process for efficiently separating anticancer active components, and the content is above 95%.
Description
technical field [0001] The present invention relates to the technical field of extracting and separating naphthoquinones from medicinal plants, and more specifically relates to a method for extracting naphthoquinones, an antitumor active ingredient from comfrey, these active ingredients not only have antitumor effects, but also have Anti-inflammatory, antipyretic, analgesic, sedative, anti-pathogenic microorganisms, anti-fertility, liver protection, etc., can be widely used in the medical and health industry. Background technique [0002] Comfrey is the dried root of Xinjiang comfrey Arnebaieuchroma (Royle) Johnst, a plant of the family Boraginaceae. The main medicinal ingredients in comfrey are naphthoquinone pigments, including shikonin, deoxyshikonin, β, β-dimethylpropylene Acylshikonin, acetylshikonin, etc. The active ingredients of naphthoquinones are isolated from comfrey, and its pharmacological effects not only have anti-pathogenic microorganism effects, anti-virus ...
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IPC IPC(8): A61K36/30A61P35/00A61K135/00
Inventor 袁晓袁萍
Owner WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI