Pleurotus nebrodensis polysaccharide-containing moisturizing cosmetic and preparation method thereof

A technology of Linggu polysaccharides and Bailing mushrooms, applied in the field of optimized preparation of Bailing mushroom polysaccharides, can solve problems such as low efficiency, destruction of polysaccharide three-dimensional structure, and changes in biological activity

Active Publication Date: 2011-11-02
SHANGHAI INOHERB COSMETIC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Bailing mushroom polysaccharide can be extracted by acid extraction, alkali extraction, hot water extraction and other methods, but the water extraction method takes a long time and has low efficiency, and the acid-base method can easily lead to the destruction of the three-dimensional structure of the polysaccharide and the change of biological activity
In addition, traditional extraction methods use dried fruiting bodies or fermented mycelium of Bailing mushroom as raw materials, which need to be dried or fermented, which may cause damage to the polysaccharide structure

Method used

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  • Pleurotus nebrodensis polysaccharide-containing moisturizing cosmetic and preparation method thereof
  • Pleurotus nebrodensis polysaccharide-containing moisturizing cosmetic and preparation method thereof
  • Pleurotus nebrodensis polysaccharide-containing moisturizing cosmetic and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0024] The optimization of embodiment 1 Bailing mushroom polysaccharide extraction conditions

[0025] (1) Determination of polysaccharide content

[0026] Accurately weigh 80.0 mg of anhydrous glucose dried at 105°C to constant weight, dissolve in distilled water and make up to 100 ml, then dilute 10 times to prepare a 0.08 mg / ml standard solution. Accurately draw 0.5ml, 1.0ml, 1.2ml, 1.5ml, 2.0ml of the standard solution, put them in dry test tubes respectively, add 2.0ml of distilled water, shake well, add 0.2% anthrone sulfate 4.0ml, shake well quickly. After 45 minutes, use 2.0ml of distilled water plus 4.0ml of chromogen as a blank, and measure at 625nm respectively. Draw a standard curve with the absorbance Y as the ordinate and the concentration as the abscissa X (mg / ml). Get the regression equation Y=14.73X-0.0186, r=0.99954

[0027] Anhydrous glucose has a good linear relationship in the range of 0.02-0.08mg / ml.

[0028] Take an appropriate amount of the dried Ba...

Embodiment 2

[0042] The preparation of embodiment 2 Bailing mushroom polysaccharide

[0043] Add 500 g of fresh Bailing mushroom, add 5 L of water, crush it with a tissue grinder, and extract it in a dynamic countercurrent cycle at 75 °C (TQ type dynamic cycle countercurrent extraction equipment, Zhejiang Erle Drying Equipment Co., Ltd.) for 1.0 hour. After centrifugation, the extract was continuously passed through two neutral alumina columns (100 mesh, Sinopharm Chemical Reagent Co., Ltd., 2mm in diameter, 25cm in height) for decolorization. Concentrate under reduced pressure (temperature 70-80°C, vacuum <-0.08MPa) to extract relative density 1.05, add ethanol until the alcohol content is 85% by volume, and produce white flocculent precipitate. After the precipitate was centrifuged, it was washed twice with 85 volume % ethanol and 95 volume % ethanol respectively to obtain 5.6 g of Bailing mushroom polysaccharide.

[0044] The extraction yield of Bailing mushroom polysaccharide is 1.12%...

Embodiment 3

[0045] The preparation of embodiment 3 Bailing mushroom polysaccharide

[0046]Add 2000g of fresh Bailing mushroom to 20L of water, crush it with a tissue grinder, and then extract it in a dynamic countercurrent cycle at 90°C for 1.5 hours. After centrifugation, the extract was continuously decolorized through two neutral alumina columns (100 mesh, 100 mesh, Sinopharm Chemical Reagent Co., Ltd. 3 mm in diameter and 50 cm in height). Concentrate until the relative density of the medicinal solution is 1.07, add ethanol until the alcohol content is 85% by volume, and produce white flocculent precipitate. After the precipitate was centrifuged, it was washed twice with 85 volume % ethanol and 95 volume % ethanol respectively to obtain 30.8 g of Bailing mushroom polysaccharide.

[0047] The extraction yield of Bailing mushroom polysaccharide is 1.54% by weight. The water content of the fruiting body of Bailing mushroom is about 80% by weight. Therefore, the extraction yield is ab...

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Abstract

The invention relates to an optimal preparation method of pleurotus nebrodensis polysaccharide, an application of the pleurotus nebrodensis polysaccharide as a moisturizer and a cosmetic with the pleurotus nebrodensis polysaccharide as the moisturizer. 0.1-2% of pleurotus nebrodensis polysaccharide is added into day cream, night cream, a facial mask and moisturizing lotion, and obvious moisturizing action can be realized. The optimal preparation method of the pleurotus nebrodensis polysaccharide comprises the following steps of: a) adding water into a pleurotus nebrodensis raw material, crushing, and carrying out dynamic countercurrent circuit extraction; and b) decolouring extract obtained by the step a), concentrating liquid medicine until relative density is 1.05-1.10, and adding ethanol until white flocculent precipitate is produced, wherein the precipitate is namely the pleurotus nebrodensis polysaccharide .

Description

technical field [0001] The invention relates to an optimized preparation method of Bailing mushroom polysaccharides. In the method, fresh Bailing mushrooms are crushed, subjected to dynamic countercurrent circulation extraction, aluminum oxide decolorization and alcohol analysis to obtain Bailing mushroom polysaccharides. It also relates to the use of Bailing mushroom polysaccharide as a moisturizing agent and cosmetics using Bailing mushroom polysaccharide as a moisturizing agent. Background technique [0002] When human skin ages, water loss increases, which leads to cell shrinkage, aging, tissue loss, atrophy, and changes in histological structure and morphology, resulting in fine wrinkles on the skin. [0003] Humectants play an important role in maintaining skin moisture and repairing skin barrier function, and are widely used in cosmetics. [0004] There are many types of moisturizers, among which glycerin, silicone oil, vaseline, self-oil, lanolin, urea, etc. are the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K8/73A61K8/891A61Q19/00
Inventor 张彤封帅
Owner SHANGHAI INOHERB COSMETIC
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