Method for preparing cordycepin esterified derivative by biocatalysis
A technology for biocatalysis and cordycepin, which is applied in the field of biocatalysis to prepare esterified derivatives of cordycepin, can solve the problems of by-products and products are difficult to separate, poor in selectivity, environmental pollution, etc., and achieves simple and easy-to-control reaction process, easy-to-use products, Environmentally friendly effect
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Embodiment 1
[0019] Put 0.5g cordycepin, 22.5ml acetone, 20mg lipase from Candida antarctica (10,000U / g, Novozymes China Biotechnology Co., Ltd.) and 1.5ml vinyl acetate into the stoppered triangle Place in the bottle, shake in a water bath constant temperature oscillator at 30°C and 150rpm, react for 6 hours, filter to remove lipase, evaporate under reduced pressure (0.02MPa) to remove the organic medium; dissolve the residue with ethanol, and then reduce pressure (0.02MPa) Ethanol was removed by evaporation, and vacuum-dried (35°C, 0.06MPa) to obtain 5′-O-acetyl cordycepin ester derivative C 12 h 15 N 5 o 4 [ 13 C NMR: C6156.2; C2152.4; C4148.9; C8139.7; C5119.1; C1'90.8; C4'77.6; C2'74.6; ); 20.7 (-CH3)], yield 66%.
Embodiment 2
[0021] Put 0.5g cordycepin, 30ml acetone, 20mg lipase from Candida antarctica (10,000U / g, Novozymes China Biotechnology Co., Ltd.) and 3ml vinyl acetate into a stoppered Erlenmeyer flask , placed in a water bath constant temperature oscillator at 30°C and 150rpm, and reacted for 12 hours, filtered to remove lipase, and evaporated to remove the organic medium under reduced pressure (0.02MPa); the residue was dissolved in ethanol, and evaporated to remove under reduced pressure (0.02MPa) Ethanol, vacuum drying (35°C, 0.06MPa) to obtain 5'-O-acetyl cordycepin ester derivative C 12 h 15 N 5 o 4 [ 13 C NMR: C6156.2; C2152.4; C4148.9; C8139.7; C5119.1; C1'90.8; C4'77.6; C2'74.6; ); 20.7 (-CH3)], yield 70%.
Embodiment 3
[0023] Put 0.5g cordycepin, 42.5ml acetone, 100mg lipase from Candida antarctica (10,000U / g, Novozymes China Biotechnology Co., Ltd.) and 5ml vinyl acetate into a stoppered Erlenmeyer flask placed in a water bath constant temperature oscillator at 30°C and 150rpm, and reacted for 24 hours, filtered to remove lipase, and evaporated under reduced pressure (0.02MPa) to remove the organic medium; the residue was dissolved in ethanol, and then evaporated under reduced pressure (0.02MPa) Remove ethanol and dry in vacuum (35°C, 0.06MPa) to obtain 5′-O-acetyl cordycepin ester derivative C 12 h 15 N 5 o 4 [ 13 C NMR: C6156.2; C2152.4; C4148.9; C8139.7; C5119.1; C1'90.8; C4'77.6; C2'74.6; ); 20.7 (-CH3)], yield 92%.
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