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Pharmaceutical composition for treating lung cancer

A composition and drug technology, applied in the field of medicine, can solve problems such as tumor recurrence and high toxicity of lung cancer

Active Publication Date: 2013-06-26
KPC PHARM INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Conventional chemotherapy drugs are highly toxic in the treatment of lung cancer and can easily lead to tumor recurrence. It is of great significance to find drugs and methods that can specifically kill lung cancer cells with low toxicity

Method used

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  • Pharmaceutical composition for treating lung cancer
  • Pharmaceutical composition for treating lung cancer
  • Pharmaceutical composition for treating lung cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: Determination of the inhibitory effect of artemether and cisplatin alone and in combination on 3LL cells

[0024] Experimental materials: DMEM / F12 medium was purchased from Hyclone Company in the United States; fetal bovine serum was purchased from Hangzhou Sijiqing Bioengineering Materials Co., Ltd.; thiazole blue (MTT) was purchased from AMRESCO Company in the United States.

[0025] Main instruments: carbon dioxide incubator (Forma3164, USA), microplate reader (Type 550, Bio-Rad, USA).

[0026] Cell line and cell culture: mouse lung cancer cell line 3LL, with DMEM / F12 medium containing 10% fetal bovine serum, penicillin and streptomycin (100u / ml each), at 37°C, 5% CO2 incubator for routine nourish.

[0027] Drugs: Artemether (ART) was provided by Kunming Pharmaceutical Group Co., Ltd.; Cisplatin (DDP) was purchased from Shandong Dezhou Pharmaceutical Factory

[0028] Experimental method: Tetramethylazolium salt method (MTT method):

[0029] Take 3LL cel...

Embodiment 2

[0046] Example 2: Observation of the morphological changes of artemether and cisplatin alone and in combination on 3LL cells

[0047] Main reagents: DMEM / F12 medium was purchased from Hyclone Company in the United States; fetal bovine serum was purchased from Hangzhou Sijiqing Bioengineering Materials Co., Ltd.

[0048] Main instruments: carbon dioxide incubator (Forma 3164, USA), inverted microscope (DMIRB, Leica, Germany).

[0049] Cell line and cell culture: mouse lung cancer cell line 3LL, with DMEM / F12 medium containing 10% fetal bovine serum, penicillin and streptomycin (100u / ml each), at 37°C, 5% CO2 incubator for routine nourish.

[0050] Drugs: Artemether (ART) was provided by Kunming Pharmaceutical Group Co., Ltd.; Cisplatin (DDP) was purchased from Dezhou Pharmaceutical Factory in Shandong Province.

[0051] experimental method:

[0052] Take 1 × 10 cells in logarithmic growth phase 6 / ml was inoculated in a 6-well plate, and the experiment consisted of a contro...

Embodiment 3

[0054] Example 3: Determination of the effects of artemether and cisplatin alone and in combination on apoptosis of 3LL cells

[0055] Reagents: DMEM / F12 medium was purchased from Hyclone Company in the United States; fetal bovine serum was purchased from Hangzhou Sijiqing Bioengineering Materials Co., Ltd.; flow-through DNA detection kit and TUNEL kit were purchased from BACKMAN COULTER Company in the United States.

[0056] Instruments: carbon dioxide incubator (Forma 3164, USA), flow cytometer (EPICSXL, 4CIR, Beckman, USA).

[0057] Cell line and cell culture: mouse lung cancer cell line 3LL, with DMEM / F12 medium containing 10% fetal bovine serum, penicillin and streptomycin (100u / ml each), at 37°C, 5% CO2 incubator for routine nourish.

[0058] Drugs: Artemether (ART) was provided by Kunming Pharmaceutical Group Co., Ltd.; Cisplatin (DDP) was purchased from Shandong Dezhou Pharmaceutical Factory

[0059] experimental method:

[0060] Flow cytometry was used for DNA ploi...

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Abstract

The invention which relates to the field of medicines concretely discloses a pharmaceutical composition for treating lung cancer. The pharmaceutical composition comprises artemether and cisplatin. Cellar tests show that the combination of artemether and cisplatin can synergistically inhibit the growth of lung cancer 3LL cell strains of a mouse, effects of singular usages of two medicines (artemether and cisplatin) and the combination of the two medicines depend on the concentration and the time, and CI is less than 1 when the two medicines with high concentration are combinatively used; the typical apoptotic morphology of 3LL cells can be observed under an inverted microscope; and a flow cytometry shows that obvious apoptosis peaks of the 3LL cell appear when the two medicines are combinatively used, and the apoptosis rate of the combination of the two medicines is higher than apoptosis rates of singular usage of artemether or cisplatin. Animal experiments show that the combination ofartemether and cisplatin performs better tumor inhibitory effects than the singular usage of cisplatin or artemether.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a pharmaceutical composition for treating lung cancer. Background technique [0002] Lung cancer is one of the most common malignant tumors in the world, with a high mortality rate. Most patients have metastases when they are diagnosed, and the 5-year survival rate is maintained at about 15%. Conventional chemotherapeutic drugs in the treatment of lung cancer are highly toxic and easily lead to tumor recurrence. It is of great significance to find low toxicity and specific drugs and methods to kill lung cancer cells. [0003] Recent studies have shown that the anti-malarial drug artemisinin and its derivatives have significant anti-tumor effects, and their pharmacological effects have received extensive attention. Artemether is one of the main derivatives of artemisinin, and its antimalarial effect is better than that of artemisinin. In recent years, more and more experiments have prove...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K33/24A61K31/357A61P35/00
Inventor 陈云建杨兆祥刘一丹刘国光杨旭娟蒋永新李高峰
Owner KPC PHARM INC