Suppression polymerase chain reaction kit
A technology of chain reaction and polymerase, which is applied in the field of inhibition of polymerase chain reaction detection kits and polymerase chain reaction kits, and can solve the problem of reducing non-specific amplification, false negatives, reduction of specific amplification efficiency, etc. problem, to achieve the effect of improving specificity and reducing concentration
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment
[0012] 1. Materials
[0013] Using the routine salting-out method in our laboratory, 30 samples of normal human peripheral blood DNA were extracted as templates to be amplified.
[0014] Primer design: according to the sequence of the 5' untranslated region of the fragile X mental retardation 1 gene (fragile X mental retardation 1, FMR1) (GenBanK S74494 and Nature Genet, 8 (1) 88-94, 1994), the following specific inner and outer sides were synthesized Primers, where the non-shaded part is the FMR1-specific sequence, which is complementary to the template, and the shaded part is the reverse complementary sequence to its own sequence:
[0015] Upstream suppression primer P1: 5'-CCA CCG GAA GGT GAT CAC CTT CCG GTG GAG-3' (SEQ ID NO: 1);
[0016] Downstream inhibitory primer P2: 5'-GGC TGA AGA GAA GCT TCT CTT CAG CCC TG-3' (SEQ ID NO: 2);
[0017] 2. Inhibition of the reaction of the PCR detection kit
[0018] S-PCR: Mix 50ul reaction system, containing 10pmol of inner and oute...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com