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Application of hypoglycemic drugs in the preparation of intestinal flora regulators

A technology of intestinal flora and regulators, applied in drug combinations, antibacterial drugs, pharmaceutical formulations, etc., can solve problems affecting the quality of life of patients

Inactive Publication Date: 2011-11-30
SHANGHAI QIBAO HIGH SCHOOL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] Diabetic patients need to take hypoglycemic drugs such as metformin, rosiglitazone, acarbose, etc. for a long time. Will these hypoglycemic drugs have a certain impact on the intestinal flora of diabetic patients while controlling blood sugar, and then affect Patient's quality of life? At present, there are few reports on the relationship between hypoglycemic drugs and intestinal flora

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  • Application of hypoglycemic drugs in the preparation of intestinal flora regulators
  • Application of hypoglycemic drugs in the preparation of intestinal flora regulators
  • Application of hypoglycemic drugs in the preparation of intestinal flora regulators

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Experimental program
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preparation example Construction

[0025] 3. Preparation of drug-containing medium

[0026] According to the reference literature report, appropriate amount of metformin, rosiglitazone, and acarbose were taken by weighing three kinds of hypoglycemic drugs respectively, placed in the above-mentioned three kinds of selective medium, and prepared into a suitable concentration (120mg / ml for metformin; Rosiglitazone is 5 mg / ml; acarbose is 15 mg / ml), and prepared as a selective medium containing hypoglycemic drugs.

[0027] 4. The effect of different dilutions of intestinal microorganisms on the experiment

[0028] Dilute the nutrient broth containing the main microorganisms in the intestinal tract to 101-104 times, inoculate 50 μl of samples of each dilution on the blank selective medium for cultivation, and make three parallels for each dilution plate. Finally, count the colonies to determine the appropriate dilution of intestinal microorganisms.

[0029] 5. Effect of hypoglycemic drugs on the main microorganis...

Embodiment 1

[0035] Example 1 Effect of Hypoglycemic Drugs on Enterobacteriaceae

[0036] 1.1 Selection of Enterobacter sample dilution

[0037] Dilute the nutrient broth containing Enterobacteriaceae to 101-104 times, inoculate, culture, and count the colonies. The results of the experiment found that when the sample was diluted 10 or 100 times, after culture, there were large connected colonies, which could not be counted. When the sample was diluted 1000 times or 10000 times, it was a single colony form after culture. Among them, the number of colonies diluted 10000 times was more suitable for counting. Therefore, the sample of Enterobacter was diluted 10000 times for the inoculation experiment.

[0038] 1.2 Effects of three hypoglycemic drugs on Enterobacteriaceae

[0039] The diluted Enterobacteriaceae samples were inoculated on blank selective medium and drug-containing selective medium for cultivation, colonies were counted, and the effects of hypoglycemic drugs on Enterobacteriac...

Embodiment 2

[0044] Example 2 Effect of Hypoglycemic Drugs on Lactobacillus

[0045] 2.1 Selection of Lactobacillus sample dilution

[0046] Dilute the nutrient broth containing lactobacilli to 101-104 times, inoculate, culture, and count the colonies. The experimental results found that when the sample was diluted 10 and 100 times, after culture, large colonies overlapped and could not be counted. When the sample was diluted 1000 times or 10000 times, it was a single colony form after culture. Among them, the number of colonies diluted 10000 times was more suitable for counting. Therefore, the inoculation experiment was performed after diluting the Lactobacillus sample 10000 times.

[0047] 2.2 Effects of three hypoglycemic drugs on Lactobacillus

[0048] Inoculate the diluted Lactobacillus samples on blank selective medium and drug-containing selective medium for cultivation, count the colonies, and count the effects of hypoglycemic drugs on Lactobacilli. The experimental results show...

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Abstract

The invention discloses the application of three hypoglycemic drugs, metformin, rosiglitazone and acarbose, in the preparation of an intestinal flora regulator. The three hypoglycemic drugs have a dynamic regulation and balance effect on the main intestinal flora, which is beneficial to the intestinal environment of patients, will contribute to the rational drug use of diabetic patients, and provide a new method for the use of microecological preparations in diabetic patients. It has positive application value for the preparation of medicines for treating and preventing gastrointestinal diseases induced by diabetes.

Description

technical field [0001] The invention relates to the application of common hypoglycemic drugs, in particular to the application of three hypoglycemic drugs, metformin, rosiglitazone and acarbose, in the preparation of intestinal flora regulators. Background technique [0002] The human body carries 1014 kinds of bacteria with a total weight of about 1271g. These bacteria begin to colonize after birth and maintain a dynamic balance of number and species. Most of the normal microbiota are colonized in the intestine, they participate in the growth and development of the host, promote the maturation of the immune system, provide nutrition for the host, and so on. Therefore, this micro-ecological system has also become an integral part of the host physiological system. [0003] Under normal circumstances, there are more than 400 kinds of bacteria in the human gastrointestinal tract, which are distributed in different parts of the intestinal tract in a certain number and proporti...

Claims

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Application Information

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IPC IPC(8): A61K31/155A61K31/4439A61K31/7036A61P1/04A61P1/12A61P1/10A61P31/04
CPCY02A50/30
Inventor 裴明
Owner SHANGHAI QIBAO HIGH SCHOOL