A kind of preparation method and application of anticancer compound
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A compound, methanol technology, applied in the field of preparation of anti-cancer compounds, can solve the problems of toxic and side reactions, high concentration, low killing rate of cancer cells, etc.
Active Publication Date: 2011-11-30
SOUTH CHINA UNIV OF TECH
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However, traditional antineoplastic drugs have a low killing rate on cancer cells
Method used
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Embodiment 1
[0023] (1) Heat the dried leaves of Xihuangcao and 1 times the weight of 50% ethanol (v / v) to boiling, reflux for extraction for 1 hour, filter to obtain the supernatant, and concentrate the supernatant to 50% of the original volume under reduced pressure to obtain the concentrate , freeze-dried powder;
[0024] (2) Dissolve the powder in water to a concentration of 5 mg / mL, pass through a D101 macroporous resin column, elute with 70% (v / v) ethanol solution, collect the eluate, and freeze-dry to obtain powder A;
[0025] (3) Dissolve powder A in water to a concentration of 5 mg / mL, pass through HP-20 macroporous resin column, elute with 70% (v / v) ethanol solution, collect the eluate, and freeze-dry to obtain powder B;
[0026] (4) Dissolve sample B in methanol so that the concentration reaches 5 mg / mL, and use a reversed-phase C18 column for gradient elution. The detection wavelength of the ultraviolet detector is 240nm. Collect 40% water and 60% methanol eluted Compound Pea...
Embodiment 2
[0030] (1) Heat the dried leaves of Xihuangcao and 4 times the weight of 60% ethanol (v / v) to boiling, reflux and extract for 2 hours, filter to obtain the supernatant, and concentrate the supernatant to 70% of the original volume under reduced pressure to obtain the concentrate , freeze-dried powder;
[0031] (2) Dissolve the powder in water to a concentration of 30 mg / mL, pass through a D101 macroporous resin column, elute with 90% (v / v) ethanol solution, collect the eluate, and freeze-dry to obtain powder A;
[0032] (3) Dissolve powder A in water to a concentration of 30 mg / mL, pass through a HP-20 macroporous resin column, elute with 90% (v / v) ethanol solution, collect the eluate, and freeze-dry to obtain a powder B;
[0033] (4) Dissolve sample B in methanol so that the concentration reaches 50 mg / mL, use a reversed-phase C18 column for gradient elution, and the detection wavelength of the ultraviolet detector is 260nm, collect 40% water and 60% methanol eluted Compoun...
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Abstract
The invention relates to a preparation method and application of an anticancer compound. The preparation method comprises the following steps of: heating dry serrate rabdosia herb leaves and ethanol in an amount which is 2 to 4 times weight of the dry serrate rabdosia herb leaves until boiling, performing refluxing extraction, filtering to obtain supernate, concentrating under reduced pressure to make the concentrated supernate 50 to 70 percent of the original supernate to obtain a concentrated solution, and freeze-drying to obtain powder; dissolving the power in water, loading on a D101 macroporous resin column, eluting by using an ethanol solution, collecting eluant, freeze-drying to obtain powder A; dissolving the powder A in water, loading on an HP-20 macroporous resin column, eluting by using a 70 to 90 percent ethanol solution, collecting eluant, and freeze-drying to obtain powder B; and dissolving the sample B in methanol to ensure that the concentration is 5-50mg/ml, performing gradient elution by using a reversed phase C18 column, collecting a compound peak eluted by 40 percent of water and 60 percent of methanol. The compound is applied to preparation of antitumor medicines, and has toxicity for human liver cancer cell lines, human leukemia cell lines and human breast cancer cell lines.
Description
technical field [0001] The invention relates to a preparation method of a compound, in particular to a preparation method and application of an anticancer compound. Background technique [0002] Tumor is a new organism formed by the body under the action of various carcinogenic factors when a certain cell in a local tissue loses its normal regulation of its growth at the gene level, resulting in abnormal clonal proliferation. It is generally believed that tumor cells are monoclonal, that is, all tumor cells in a tumor are descendants of a mutant cell. Tumors are generally divided into benign and malignant. All malignant tumors are collectively called cancer. At present, the death caused by malignant tumor is the second leading cause of human death after cardiovascular disease. [0003] Tumor treatment methods include surgery, radiotherapy, and drug therapy (ie chemotherapy), among which chemotherapy is currently the main treatment. Chemotherapy can cure some tumor patien...
Claims
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