Fluorescent bioprobes for upconversion nanoparticle-labeled aptamers
A fluorescent bioprobe and nanoparticle technology, applied in the field of fluorescent bioprobes, can solve problems such as high background signal, biological nucleic acid damage, potential toxicity, etc., achieve low background signal, avoid damage, and improve detection sensitivity
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specific Embodiment approach 1
[0019] Specific implementation mode one: combine figure 2 To illustrate this embodiment, the fluorescent bioprobe for upconversion nanoparticle-labeled aptamer includes an aptamer labeled with a fluorescent donor, and a DNA strand complementary to the aptamer. The FRET effect occurs as the dye molecule of the fluorescent acceptor, and the UCNPs are used as the fluorescent donor. The UCNPs described in this embodiment are NaYF 4 :Yb, Er, NaYF 4 :Yb, Tm, NaYF 4 :Yb, Ho, LaF 3 :Yb, Er, LaF 3 :Yb, Tm or LaF 3 : one of Yb, Ho, the UCNPs described in the present embodiment are water-soluble, and the UCNPs described in the present embodiment are connected with the aptamer with avidin and biotin.
specific Embodiment approach 2
[0020] Specific implementation mode two: combination figure 2 and image 3 Describe this embodiment, this embodiment is the preparation method of the fluorescent bioprobe of the upconversion nanoparticle-labeled aptamer described in the first embodiment:
[0021] (1) Preparation of UCNPs: 0.272 g of CF 3 COONa, 0.376 g Y(CF 3 COO) 3 , 0.113 g Yb (CF 3 COO) 3 and 0.055g of Er(CF 3 COO) 3 Dissolve it in 10ml of oleylamine, put it into a three-necked bottle, and stir at 100°C for 30 minutes under the protection of argon, then gradually raise the temperature to 320°C and keep it for 1 hour. The product was separated by centrifugation and ultrasonically washed with cyclohexane to obtain a cyclohexane solution of UCNPs.
[0022] (2) Utilize aminophosphoric acid to carry out aqueous phase transfer to the UCNPs prepared in step (1): Weigh 0.13 gram of aminophosphoric acid and disperse it in 5ml ethanol, drop the cyclohexane solution of 3ml UCNPs therein, stir at room temperat...
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