Preparation method of chitosan/hydroxyapatite three-dimensional porous scaffold material

A hydroxyapatite, three-dimensional porous technology, applied in the field of materials, can solve the problems of irregular pores of the scaffold material, exerting a large extrusion force, fragmentation of the porogen, etc., and achieves good effect, convenient operation and simple method. Effect

Inactive Publication Date: 2011-12-07
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Tetsuo A. Use the extrusion method to arrange the dense packing of amorphous NaCl and sucrose. The method is simple, easy to operate, and the dense packing effect is good. However, since NaCl and sucrose are used as porogens, in order to achieve the greatest degree of close packing , needs to apply a lot of extrusion force, which will lead to the fragmentation of some porogens with uniform particle size, and the scaffold material formed after leaching has irregular pores and non-uniform pore size.

Method used

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  • Preparation method of chitosan/hydroxyapatite three-dimensional porous scaffold material
  • Preparation method of chitosan/hydroxyapatite three-dimensional porous scaffold material
  • Preparation method of chitosan/hydroxyapatite three-dimensional porous scaffold material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 1. Dissolving chitosan and hydroxyapatite

[0035] Dissolve 1g chitosan (deacetylation degree is 80%~95%), 0.1g hydroxyapatite in the acetic acid aqueous solution of 100mL 20g / L, stir for 6 hours, centrifuge to remove air bubbles, obtain chitosan and hydroxyapatite stone mixture.

[0036] 2. Preparation of chitosan / hydroxyapatite three-dimensional porous composite scaffold material

[0037] SiO 2 Microspheres are porogens, SiO with a particle size of 100-150 μm 2 Add the microspheres into a 20 mL syringe with a needle, squeeze and tamp the SiO with a pestle 2 Microspheres until their position in the syringe does not change, SiO 2 The amount of microspheres added is 0.25 of the volume of the syringe, and then the mixed solution of chitosan and hydroxyapatite is added to the syringe, and the added amount of the mixed solution is the same as that of SiO 2 The volume of the microspheres is the same, pushing the plunger handle of the syringe makes the mixed solution flo...

Embodiment 2

[0044] In the preparation chitosan / hydroxyapatite mixed solution step 1 of embodiment 1, 1g chitosan (deacetylation degree is 80%~95%), 0.2g hydroxyapatite are dissolved in 100mL 20g / L in aqueous acetic acid solution, stirred for 6 hours, and centrifuged to remove air bubbles. The other steps were the same as in Example 1, and a chitosan / hydroxyapatite three-dimensional porous scaffold material was prepared.

[0045] According to the method of test 1, the morphology characterization, porosity and compressive strength test were carried out. The experimental results showed that the pores of the composite porous scaffold were open, the connectivity between the pores was good, and the average pore diameter was 117 μm (see figure 2 ), the porosity is 91.97%, and the compressive strength is 818kPa.

Embodiment 3

[0047] In step 1 of dissolving chitosan and hydroxyapatite in the present embodiment, 1 g of chitosan (the degree of deacetylation is 80% to 95%) and 0.05 g of hydroxyapatite are dissolved in 100 mL of 20 g / L acetic acid aqueous solution , stirred for 4 hours, and centrifuged to remove air bubbles. The other steps were the same as in Example 1, and a chitosan / hydroxyapatite three-dimensional porous scaffold material was prepared.

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Abstract

The invention discloses a preparation method of a chitosan / hydroxyapatite three-dimensional porous composite bracket material. Chitosan / hydroxyapatite three-dimensional porous composite brackets of different pore diameters are prepared by taking high-purity SiO2 microspheres with uniform particle sizes and porous surfaces as pore-foaming agents and adjusting the pore space of a porous bracket material in a way of changing the sizes of the SiO2 microspheres based on a solvent pouring-particle leaching technology in combination with an extruding method. Compared with the conventional method forarranging the pore-foaming agents in a close packing way with a bonding agent, the invention has the advantages that: the method is easy and convenient to operate; the pore-foaming agent close packing effect is good; and the prepared porous bracket is free from the residual bonding agent, has high permeability, uniform particle size and higher pore space and compression strength than a pure chitosan porous bracket. As proved by a test result, the bracket has high in-vitro and in-vivo biological compatibility.

Description

technical field [0001] The invention belongs to the technical field of materials, and in particular relates to a preparation method of a porous support material. Background technique [0002] The extracellular matrix (Extra Cellular Matrix, ECM) plays an important role in tissue regeneration. It not only has physical functions such as connection, support, water retention, compression resistance and protection, but also plays a full range of biological functions on the basic life activities of cells. It affects the survival, growth and death of cells, determines the shape of cells, controls cell differentiation, participates in cell migration, etc. Therefore, the main goal of preparing scaffolds is to simulate the function of natural ECM in a temporary three-dimensional space, so that Scaffolds guide every cellular response, including cell adhesion, movement, division, and phenotypic selection. [0003] The selection and preparation of tissue engineering scaffold materials i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/56A61L27/20A61L27/12
Inventor 张志琪王金磊牟朝丽祁小妮
Owner SHAANXI NORMAL UNIV
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