Ultrasonic Vibration Microscopic Cell Penetration System Based on Piezoelectric Ceramics

Abstract

The invention discloses a supersonic vibration microscopic cell penetrating system based on piezoelectric ceramic driving. According to the invention, supersonic vibration characteristics of high energy and high efficiency are utilized. A procedure of stabbing a sinking-tip injection needle through a surface of an embryonic cell in a traditional microscopic injection operation is changed into a procedure of supersonic vibration interaction carried out by using a flat-tip injection needle upon a cell surface. During the procedure, cell surface tissues serve as supersonic vibration energy transmission media. When configured supersonic vibration energy exceeds a threshold of energy that the cell surface tissue can bear, a small area of rupture appears on tissues affected by the needle tip, such that the injection needle enters the cell through the cell surface tissues easily, and internal tissue scratching and damaging caused by the sharp needle tip are avoided. Therefore, a good base isestablished for subsequent microscopic injection operations, and embryonic cell survival rate can be greatly improved.

Description

【Technical field】 [0001] The invention relates to the technical field of micro-manipulation robots, in particular to a micro-manipulation robot system oriented to biomedical engineering. 【Background technique】 [0002] In biomedical scientific research and clinical testing, cell-level micro-manipulation has attracted more and more attention. Among them, embryo cell injection technology is an important means of modern biological and medical research, especially in biological cloning and transgenic experiments, it has become the most widely used and most effective method. [0003] In biological experiments such as embryonic stem cells and cloning, the outer surface of the transgenic injected embryonic cells is more flexible, so that the injection needle with a blunt tip cannot easily pierce the outer surface of the embryonic cells and penetrate into the cell to complete the injection. At present, domestic biological research institutions mostly use the oblique opening with sh...

AI Technical Summary

Problems solved by technology

[0005] At present, the patent of Harbin Institute of Technology (patent number: 200610010025, inventors: Chen Liguo, Rong Weibin, Liu Yaxin, Wang Huixiang) describes a microdissection device based on piezoelectric ultrasonic vibration, which can flatten and evenly slice biological tissues. It overcomes the defects of high work intensity, easy fatigue, and inevitable human error when the work is done manually.

For micro-cell injection, two tasks of cell penetration and injection need to be completed, so although this invention can well solve the problem of micro-tissue cutting, it cannot be applied to micro-cell injection.

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