Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Juglans mandshurica bark extract and application thereof in preparing anticancer drugs

A technology for the extract of Chinese catalpa, which is applied to the bark extract of Chinese catalpa and its application in the preparation of anticancer drugs, and can solve the problem that the activity detection model is limited to animals, tissues, complex components of Chinese catalpa, and lack of systems and in-depth exploration to achieve the effect of simple extraction and separation methods, promotion of social population health, and enhancement of social productivity

Active Publication Date: 2011-12-14
GUANGDONG PHARMA UNIV
View PDF2 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the components in the bark of Juglans catalpa are very complex, which ones play a key role in the obtained extract, which components have the main anticancer activity, and what are their exact anticancer effects? These work are lack of system and in-depth research
In addition, the anti-tumor research on Juglans catalpa mainly focuses on natural chemistry, and the applied activity detection models are limited to animals, tissues, etc.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Juglans mandshurica bark extract and application thereof in preparing anticancer drugs
  • Juglans mandshurica bark extract and application thereof in preparing anticancer drugs
  • Juglans mandshurica bark extract and application thereof in preparing anticancer drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Take 500g of walnut tree bark (dry product), put it in a round bottom flask, add 4000ml of 70% ethanol, put it in an electric heating mantle and add a condenser to form a reflux system. Repeated reflux extraction 3 times, the first 1000ml reflux for 1h, then Two 3000ml reflux for 2.5h, a total of 4000ml of 70% ethanol solution was used for reflux extraction for 3.5h. Use a rotary evaporator to recover ethanol under reduced pressure and concentrate the extract to a thick paste, add 5 times the amount of water to dissolve and disperse. After dissolving in water, they were fractionally extracted with petroleum ether, ethyl acetate, and n-butanol, and each phase was extracted 10 times, each with 300ml, a total of 3000ml extract. The extract was distilled to dryness under reduced pressure to obtain petroleum ether component, ethyl acetate component, n-butanol component and water phase component respectively. Take a small amount of each extracted part, dissolve it in DMSO, fi...

Embodiment 2

[0034] Table 1. Petroleum ether phase, ethyl acetate phase, n-butanol phase, water relative to BEL-7402 cell IC50 results

[0035]

[0036] Note: ----- shows no significant inhibitory effect. P>0.05.

[0037]

Embodiment 3

[0039] Crush the bark of walnut tree bark, take 500g of bark powder and reflux with 70% ethanol for 3 times, the first 4000ml reflux for 3h, the last two 3000ml reflux for 2.5h, a total of 70% ethanol solution 7000ml reflux extraction for 5.5h . The extracts were combined, concentrated under reduced pressure, and the extract was collected. Dissolve with 5 times water, filter with suction, and save the filter residue. The filtrate was extracted with ether, ethyl acetate, and saturated n-butanol, and each phase was extracted 10 times with 300 ml each time. The extracts were combined and dried by rotary evaporation. After MTT cell screening test, it is calculated that the IC50 value of the ethyl acetate extract phase in the ether phase, ethyl acetate phase, n-butanol phase, and water phase is the smallest, and the anti-tumor activity is higher, so the ethyl acetate phase is selected for further subdivision .

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a Juglans mandshurica bark extract with good anticancer effect, which is prepared by the following steps: (1) extracting the Juglans mandshurica bark with 70-75 wt% alcohol solution under reflux for 3.5-5.5 hours, evaporating to recycle alcohol so as to obtain a concentrated solution, dissolving by adding water into the concentrated solution, and extracting with ethyl acetate to obtain an liquid extract; and (2) after drying the liquid extract, separating by silicagel column chromatography, and eluting with an eluting agent to obtain an eluate, wherein the volume ratio of chloroform to methanol solution in the eluting agent is 3:2-0:1. The Juglans mandshurica bark extract which is extracted and separated in the invention has better effect than other existing Juglans mandshurica bark extracts in the aspect of cancer resistance, especially liver cancer resistance. The extraction and separation method of the Juglans mandshurica bark extract provided by the invention is simple and easy to implement.

Description

technical field [0001] The invention belongs to the field of natural medicines, and in particular relates to an extract from the bark of catalpa chinensis and its application in the preparation of anticancer drugs. Background technique [0002] The research and development of natural medicines play an important role in the process of finding new medicines and fighting diseases. Statistics show that among the 175 anticancer drugs used worldwide, about 57% are directly or indirectly derived from natural products. Therefore, the prospect of finding and developing new drugs from natural plants is very broad. my country has a vast territory and abundant natural medicine resources. How to make more effective use of these natural medicine resources and develop new medicines that can benefit mankind is an important task for drug researchers. The traditional natural medicine research is generally by first extracting, separating and identifying the chemical components in plants, and...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K36/52A61P35/00A61K129/00
Inventor 张咏莉陆家政李红枝黄清松崔玉强汪向升
Owner GUANGDONG PHARMA UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com