A kind of selective amination method of bacterial cellulose

A bacterial cellulose and selective technology, applied in the field, can solve problems such as weak interaction between nanoparticles and bacterial cellulose membrane, membrane surface slipping, human hazards, etc., to achieve mild reaction conditions, avoid falling off, and help stabilize sexual effect

Inactive Publication Date: 2011-12-14
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the force between the nanoparticle and the bacterial cellulose membrane prepared by forming a complex between the hydroxyl group and the metal ion is weak, and it is easy to slide off the surface of the m

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) the bacterial cellulose produced by Acetobacter xylinum is placed in a freeze dryer and freeze-dried for 24 hours;

[0032] (2) the bacterial cellulose after freeze-drying is immersed in the aqueous solution containing 0.01mol / L 2,2,5,5-tetramethylpiperidine-N-oxyl and 0.01mol / L horseradish peroxidase, The mass-volume ratio of described bacterial cellulose in described aqueous solution is 2g / L, and with the HNO of 1mol / L 3 The solution adjusts the pH of the above aqueous solution to be 2;

[0033] (3) within 1 hour, gradually add dropwise the hypochlorous acid solution of 1mol / L to make the reaction, the amount added dropwise is one-tenth of the volume of the aqueous solution by volume, and the reaction temperature is 100°C;

[0034] (4) The bacterial cellulose treated in the previous step is taken out, washed 5 times with distilled water, and then washed 5 times with ethanol until its pH value is 7, and the selectively oxidized bacterial cellulose is obtained;

...

Embodiment 2

[0038] (1) Place the bacterial cellulose produced by Acetobacter acetogenes in a lyophilizer and freeze-dry for 24 hours;

[0039] (2) The bacterial cellulose after freeze-drying is immersed in the aqueous solution containing 0.1mol / L 4-methyl-TEMPO and 0.1mol / L bean hull peroxidase, the quality of the bacterial cellulose in the aqueous solution -The volume ratio is 5g / L, and the pH value of the above-mentioned aqueous solution is adjusted to 3 with the HCl solution of 5mol / L;

[0040] (3) Gradually drop the hypochlorite solution of 2mol / L in 2 hours to make the reaction, the dripping amount is one-tenth of the volume of the aqueous solution by volume, and the reaction temperature is 20°C;

[0041] (4) The bacterial cellulose treated in the previous step is taken out, washed 3 times with distilled water, and then washed 3 times with ethanol until its pH value is 6, and the selectively oxidized bacterial cellulose is obtained;

[0042] (5) Add the above-mentioned selectively o...

Embodiment 3

[0045] (1) the bacterial cellulose produced by Acetobacter is placed in a freeze dryer and freeze-dried for 24 hours;

[0046] (2) The bacterial cellulose after freeze-drying is immersed in the aqueous solution containing 0.51mol / L 4-hydroxyl-TEMPO and 0.5mol / L myeloperoxidase, the mass-volume of described bacterial cellulose in described aqueous solution The ratio is 10g / L, and 5mol / L of HNO 3 The solution adjusts the pH value of the above aqueous solution to 4, and the reaction temperature is 90°C;

[0047] (3) within 3 hours, the chlorous acid solution of 5mol / L was gradually added dropwise to make the reaction, and the amount of addition was one-tenth of the volume of the aqueous solution by volume;

[0048] (4) The bacterial cellulose treated in the previous step is taken out, washed 5 times with distilled water, and then washed 3 times with ethanol until its pH value is 6, and the selectively oxidized bacterial cellulose is obtained;

[0049] (5) Add the above-mentione...

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PUM

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Abstract

The invention relates to a selective amination method of bacterial cellulose and in particular relates to a bacterial cellulose selective amination method, which is to oxidize primary hydroxyl on C6 of anhydroglucose ring of the bacterial cellulose into carboxylic acid under an acidic condition by adopting a nitroxyl/enzyme co-oxidant system, and performing amination. The method comprises the following steps: (1) placing the lyophilized bacterial cellulose into the nitroxyl/enzyme system solution, regulating the pH to be acidic, slowly adding an oxidant, regulating the pH to be neutral, and cleaning to obtain selectively oxidized bacterial cellulose; and (2) placing the selectively oxidized bacterial cellulose into a diamine substance solution, heating to react for 2 to 4 hours, and washing to obtain the selectively aminated bacterial cellulose. According to the selective amination method of bacterial cellulose, the primary hydroxyl on C6 of the anhydroglucose ring of the bacterial cellulose can be selectively converted into amidogen completely and effectively, and can provide a chelation group to bacterial cellulose supported functional particles.

Description

technical field [0001] The invention relates to a selective amination method of bacterial cellulose, in particular to a method for the amination of the primary hydroxyl group at the C6 position of the anhydroglucose ring of bacterial cellulose, specifically a method using nitroxyl / enzyme co-oxidant The system oxidizes the primary hydroxyl group on the C6 position of the anhydroglucose ring of bacterial cellulose to carboxylic acid under acidic conditions, and then performs the selective amination method of bacterial cellulose for amination. Background technique [0002] Bacterial cellulose (BC for short) is a kind of cellulose produced by some bacteria. In order to distinguish it from the cellulose produced by plants, it is also called microbial cellulose. Bacterial cellulose is a new biological material with excellent performance, which has been extensively studied in recent years. Although the composition of bacterial cellulose is similar to general cellulose, its physica...

Claims

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Application Information

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IPC IPC(8): C08B15/06C12P19/14
Inventor 王宝春张雯周碧辉胡伟立洪枫陈仕艳王华平
Owner DONGHUA UNIV
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